We narrowed to 5,806 results for: pCas

1. SpCas9_sgRNA_expression_in_pBluescript

   Plasmid

   #122089

   Purpose

   U6 driven SpCas9 sgRNA expression vector for cloning own guides

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGmRuby2smFPV5

   Plasmid

   #71817

   Purpose

   encodes multiple copies of an epitope tag V5( not detected by anti GFP ab)

   Depositor

   Loren Looger

   Insert

   mRuby2_smFP_V5
   

   Tags

   V5

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

3. pCAG:mRFP1

   Plasmid

   #32600

   Depositor

   Anna-Katerina Hadjantonakis

   Article

   Long et al BMC Biotechnol. 2005 Jul 4;5:20.

   Insert

   mRFP1
   

   Expression

   Mammalian

   Promoter

   pCAGGs

   Available Since

   Jan. 9, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pCAT207

   Plasmid

   #134884

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAT007

   Plasmid

   #171634

   Purpose

   Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and CAG promoter expressing mTagBFP2

   Depositor

   Jennifer Doudna

   Article

   Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

   Inserts

   spyCas9 sgRNA-TRAC
   mTagBFP2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   June 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAT224

   Plasmid

   #134892

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAM105

   Plasmid

   #86479

   Purpose

   Expresses p9-FLAG-FMO

   Depositor

   Pamela Silver

   Article

   Myhrvold et al ACS Synth Biol. 2016 Aug 3.

   Insert

   p9-FLAG-FMO
   

   Use

   Synthetic Biology

   Tags

   FLAG and FMO

   Expression

   Bacterial

   Promoter

   pTet

   Available Since

   May 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAT205

   Plasmid

   #134882

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Insert

   mRFP1
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAT220

   Plasmid

   #134888

   Purpose

   Bacterial expression

   Depositor

   Thomas Howard

   Article

   Azubuike et al N Biotechnol. 2021 Nov 25;65:20-30. doi:

   Insert

   eGFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   July 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAS9i_TRP

    Plasmid

    #141253

    Purpose

    Galactose-inducible expression of Cas9 for addressing one target; Contains guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

    Depositor

    Adam Bertl

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Promoter

    GAL1 (Cas9), pSNR52 (gRNA)

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas12a

    Plasmid

    #186446

    Purpose

    Encodes Cas12a from F. novicida (type V-A)

    Depositor

    John van der Oost

    Article

    Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

    Insert

    Cas12a
    

    Use

    CRISPR

    Promoter

    T7

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSpCas9_U6_sgRNA_Mettl3C

    Plasmid

    #165420

    Purpose

    sgRNA construct for targeting Mettl3 C-terminus

    Depositor

    Neil Brockdorff

    Article

    Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

    Insert

    PspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCasX1

    Plasmid

    #87685

    Purpose

    p15A plasmid expressing CasX locus from Deltaproteobacteria

    Depositor

    Jennifer Doudna

    Article

    Burstein et al Nature. 2016 Dec 22. doi: 10.1038/nature

    Insert

    CasX1
    

    Expression

    Bacterial

    Promoter

    None

    Available Since

    April 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAM111

    Plasmid

    #86481

    Purpose

    Expresses p9-6His-tnaA

    Depositor

    Pamela Silver

    Article

    Myhrvold et al ACS Synth Biol. 2016 Aug 3.

    Insert

    p9-6His-tnaA
    

    Use

    Synthetic Biology

    Tags

    6His and tnaA

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCA0.421

    Plasmid

    #203950

    Purpose

    Level 0 acceptor vector for assembling gRNA arrays for Cas12a in SP position

    Depositor

    Alistair McCormick

    Article

    Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Available Since

    May 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCast

    Plasmid

    #200169

    Purpose

    E. coli/Yeast shuttle vector carrying CloNAT marker and Spcas9 allowing cloning of gRNA flanked by tRNA promoter and HDV ribozyme

    Depositor

    Yong-Su Jin

    Article

    Kuanyshev et al Biotechnol Bioeng. 2021 Jan;118(1):372-3

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    June 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCas2C

    Plasmid

    #82374

    Purpose

    dCas9 expressed from aTc-inducible promoter in acsA, C RBS: TGGACA

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    dCas9
    

    Promoter

    PEZ3

    Available Since

    Sept. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCas34

    Plasmid

    #82386

    Purpose

    sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

    Depositor

    Brian Pfleger

    Article

    Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

    Insert

    gRNA
    

    Promoter

    PEZ3

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCas3

    Plasmid

    #229538

    Purpose

    pMV_hyg encoding Cas3(wt) and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning.

    Depositor

    Kevin Verstrepen

    Article

    Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

    Insert

    Cas3
    

    Use

    CRISPR

    Expression

    Bacterial and Yeast

    Mutation

    codon optimized for S.cerevisiae

    Available Since

    Jan. 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAT105

    Plasmid

    #180510

    Purpose

    Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    DpbCasX with R3 loop
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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