We narrowed to 4,786 results for: LRE
-
Plasmid#165885PurposeBlasticidin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRMCE{PuroR-5FCS-GMRWhite}-Vasa-FC31
Plasmid#165888PurposePuromycin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertionsDepositorInsertAll-in-one FC31 RMCE Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRMCE{BlastR-5FCS-GMRWhite}-Vasa-FC31
Plasmid#165889PurposeBlasticidin resistant FC31 integrase mediated RMCE cassette for upgrading MiMic insertionsDepositorInsertAll-in-one FC31 RMCE Cassette
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
P[acman]-Alpha1
Plasmid#165858PurposeAlpha1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
P[acman]-Omega1
Plasmid#165860PurposeOmega1 Basic cloning vector GB2.0 compatible P[acman]-derived backbone. Allows for copy induction from low to high using AutoFOS or similar. Requires EPI300 bacterial strain (DH10B Derived) or similar. uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pR6Kg-Alpha2
Plasmid#165863PurposeAlpha level R6K_ origin GB Cloning vector, Conditionally replicative ori, requires pir gene expression requires pir gene expression requires EC100D or EC100D116 bacterial strain (DH10B derived) uses blue-white screeningDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJS697
Plasmid#168778PurposeYeast surface display plasmid backbone that can be combined with pJS699 through homologous recombinationDepositorTypeEmpty backboneUseTagsCmyc and HAExpressionYeastMutationPromoterAvailable sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
Eomes gRNA#1
Plasmid#170834PurposeCas9-mediated knockout of Eomes in mammalian cellsDepositorInsertEomes (Eomes Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Eomes gRNA#2
Plasmid#170835PurposeCas9-mediated knockout of Eomes in mammalian cellsDepositorInsertEomes (Eomes Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
Eomes gRNA#3
Plasmid#170836PurposeCas9-mediated knockout of Eomes in mammalian cellsDepositorInsertEomes (Eomes Mouse)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceAug. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcry:mCherry,-600unc:cct3GFP
Plasmid#172528PurposealphaA-crystallin promoter (cry) drives mCherry expression in the lens. The 600bp unc-45b muscle-specific promoter drives expression of GFP-tagged zebrafish cct3.DepositorInsertcct3-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPuroR-GMRWhite
Plasmid#165884PurposePuromycin resistance vector with attB and eye marker. Contains an Alpha GB cloning entry point for addition of custom assembly uses blue-white screeningDepositorInsertTransgenesis Ready Selection Vector
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-mSNX27-H112A RNAi-resistant
Plasmid#163621Purposemouse SNX27 cDNA with point mutation to resist siRNA against SNX27 and with a point mutation H112A C-terminally tagged with GFPDepositorInsertmouse SNX27-H112A RNAi-resistant (Snx27 Mouse)
UseTagsGFPExpressionMammalianMutationH112A and RNAi resistancePromoterAvailable sinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
Donor-PIGAshort
Plasmid#153547PurposeUsed as a donor vector for the targeted correction of a mutation in PIGA exon 6DepositorInsertPIGA (a 603-bp fragment from intron 5 and exon 6)
UseDonor plasmidTagsN/AExpressionMutationNo mutationPromoterNo promoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
Donor-PIGAmut
Plasmid#153548PurposeUsed as a donor vector for the targeted knock-in of a mutation in PIGA exon 6DepositorInsertmutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6)
UseDonor plasmidTagsN/AExpressionMutationa 3-bp mutation in exon 6PromoterNo promoterAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pISH-OMACS
Plasmid#136765PurposeTemplate to synthesize riboprobes for in situ hybridization (ISH) for Acsm4DepositorInsertAcsm4 (Acsm4 Mouse)
UseIn situ probeTagsExpressionMutationISH Probe insert, A102T- please see depositor com…PromoterAvailable sinceOct. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC_h7SK M15 (UUA)
Plasmid#154785Purposeplasmid with single copy of Mma PylT M15 mutant (UUA anticodon)DepositorInsertMma PylT
UseTagsExpressionMammalianMutationUUA anticodon and G10A, G14U, G16U, C20U, C52APromoterh7SKAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
miR-223-RFP pCDNA
Plasmid#97142Purposeexpression of miR-223 and TagRFPDepositorInsertmiR-223
UseTagsTagRFPExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSI-check2-TAB2-3'UTR
Plasmid#97157PurposeFor dual luciferase report assayDepositorInsertTAB2 3'UTR (TAB2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;snx5CE1-basEgfp
Plasmid#90138Purposecontains snx5 enhancer sequence and a basal promoter driving expression of eGFPDepositorInsertsnx5E1basEgfp (snx5 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceJune 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;yrkCE1-P1Egfp
Plasmid#90156Purposecontains yrk enhancer sequence, and yrk specific gene promoter driving expression of eGFPDepositorInsertyrkE1P1Egfp (yrk Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;snx5CE1-P1Egfp
Plasmid#90152Purposecontains snx5 enhancer sequence, and snx5 specific gene promoter driving expression of eGFPDepositorInsertsnx5E1P1Egfp (snx5 Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2-cryR;yrkCE1-basEgfp
Plasmid#90142Purposecontains yrk enhancer sequence and a basal promoter driving expression of eGFPDepositorInsertyrkE1basEgfp (yrk Zebrafish)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG_muBim_Ex3
Plasmid#85533PurposeInducible expression of guide RNA (muBim_Ex3) with fluorescent GFP reporterDepositorInsertmu Bim
UseTagsExpressionMutationPromoterH1tAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-FRB-DMVF-181-N
Plasmid#87609PurposeFRB-tagged N-terminal Dronpa fragment for BiFC; localizes to plasma membrane.DepositorInsertLyn-FRB-DMVF181-N
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LifeAct-FRB-DMVF-181-N
Plasmid#87610PurposeFRB-tagged N-terminal Dronpa fragment for BiFC; localizes to actin cytoskeleton.DepositorInsertLifeAct-FRB-DMVF181-N
UseTags17-aa sequence from Abp140 (aka "Lifeact&quo…ExpressionMammalianMutationPromoterCMVAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
PWPXLD-EGFR-WT
Plasmid#133749PurposeWild-type EGFR tagged with HA cloned into lentiviral PWPXLD plasimdDepositorInsertWild type EGFR-HA (EGFR Human)
UseLentiviralTagsHA tagExpressionMammalianMutationPromoterEF1-alphaAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-NLS-CBGreen-FIRE-puro
Plasmid#139195PurposeLuminescent reporter of ERK activityDepositorInsertCBG99
UseLuciferase and RetroviralTagsFra1 PEST domain fragment (FIRE) and nuclear loca…ExpressionMammalianMutationSee depositor comments belowPromoterAvailable sinceSept. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSI-Check2-hRluc-NFkB-firefly
Plasmid#106979PurposeLuciferase reporter with internal control for NFkB signal.DepositorInsertNFKB recognition sites (NFKB1 Human)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-EGFP-T2A-Bsd
Plasmid#193198PurposeLentiviral vector expressing EGFP (driven by EFS promoter) and a blasticidin resistance markerDepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only