We narrowed to 9,222 results for: Pol

1. pUltra-Hot-PC

   Plasmid

   #184466

   Purpose

   Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A)

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Insert

   Pyruvate Carboxylase (PC Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   UbC

   Available Since

   Aug. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEMS2181

   Plasmid

   #158421

   Purpose

   AAV plasmid with Ple32 (CLDN5 MiniPromoter) driving expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple32 (CLDN5 Human)
   

   Use

   AAV

   Promoter

   Ple32

   Available Since

   Feb. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. RRL.sin.cPPT.SFFV/Flag-codon-optimized-NCOA7 variant 6.IRES-puro.WPRE (CG584)

   Plasmid

   #139444

   Purpose

   Lentiviral vector to ectopically express human codon-optimized NCOA7 isoform 4

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   FLAG-tag codon-optimized NCOA7 variant 6 (coding for isoform 4, the short, interferon-inducble isoform) (NCOA7 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   sequence codon-optimized

   Promoter

   SFFV

   Available Since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABE-neomycin-PC-MYC

   Plasmid

   #184550

   Purpose

   Retroviral vector to express human PC with MYC tag

   Depositor

   Gerardo Ferbeyre

   Article

   Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

   Insert

   Pyruvate Carboxylase (PC Human)
   

   Use

   Retroviral

   Tags

   MYC

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   June 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. BCL2alpha-MJK2243

   Plasmid

   #227690

   Purpose

   Expresses human BCL2alpha

   Depositor

   Curt Civin

   Article

   Goodis et al Eur J Med Chem. 2024 Dec 22;285:117190.

   Insert

   human BCL-2alpha (BCL2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Jan. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEMS2230

   Plasmid

   #158420

   Purpose

   AAV plasmid with Ple253 (PITX3 MiniPromoter) driving expression of EmGFP. Contains WPRE.

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

   Insert

   Ple253 (PITX3 )
   

   Use

   AAV

   Promoter

   Ple253

   Available Since

   Feb. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEN313 - Rad21-Halo-Frt-PGK-EM7-NeoR-bpA-Frt targeting

   Plasmid

   #156431

   Purpose

   Targeting vector to introduce an Halotag cassette at the mouse Rad21 locus using NEOMYCINE selection. Designed for using with sgRNA CCACGGTTCCATATTATCTG

   Depositor

   Elphege Nora

   Article

   Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

   Insert

   RAD21, Halotag (Rad21 Mouse)
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Mutation

   none

   Available Since

   Sept. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUDP013

   Plasmid

   #103873

   Purpose

   Broad-host-range Cas9/gRNA co-expression plasmid with gRNA targetting ADE2 from Ogataea (para)polymorpha

   Depositor

   Jean-Marc Daran

   Article

   Juergens et al FEMS Yeast Res. 2018 May 1;18(3). pii: 4

   Insert

   HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
   

   Use

   CRISPR

   Expression

   Yeast

   Promoter

   ScTDH3

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTWIN1-His6-Ssp-Httex1-23Q-A2C-A60C

   Plasmid

   #114613

   Purpose

   Bacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 23 polyQ repeat

   Depositor

   Hilal Lashuel

   Article

   Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

   Insert

   Huntingtin Exon1 (HTT Human)
   

   Tags

   His6-Ssp DnaB intein

   Expression

   Bacterial

   Mutation

   A2C, A60C; 23 polyQ repeat

   Available Since

   Sept. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTWIN1-His6-Ssp-Htt2-90-43Q-P90C

    Plasmid

    #114626

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 43 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    P90C; 43 polyQ repeat

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTWIN1-His6-Ssp-Htt2-90-15Q-P90C

    Plasmid

    #114611

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 15 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    P90C; 23 polyQ repeat

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTWIN1-His6-Ssp-Htt2-90(A2C)-37Q

    Plasmid

    #114617

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 37 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C; 23 polyQ repeat

    Available Since

    Oct. 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pTWIN1-His6-Ssp-Httex1-43Q-A2C

    Plasmid

    #114622

    Purpose

    Bacterial expression of Htt fragment containing exon 1, with A2C cysteine mutation and a 43 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 (HTT Human)
    

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C; 43 polyQ repeat

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTWIN1-His6-Ssp-Httex1-43Q-A2C-A60C

    Plasmid

    #114623

    Purpose

    Bacterial expression of Htt fragment containing exon 1, with A2C & A60C cysteine mutations and a 43 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 (HTT Human)
    

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, A60C; 43 polyQ repeat

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q

    Plasmid

    #114627

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C cysteine mutation and a 49 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C; 49 polyQ repeat

    Available Since

    Sept. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTWIN1-His6-Ssp-Htt2-90-49Q-P90C

    Plasmid

    #114631

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with P90C cysteine mutation and a 49 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    P90C; 49 polyQ repeat

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSN-A112C-1A32

    Plasmid

    #191241

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A32 and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    

    Tags

    nuclease A from S. aureus fused to the poly-Leu T…

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and predic…

    Promoter

    T7 promoter

    Available Since

    Feb. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSN-A112C-1A31

    Plasmid

    #191242

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-1A31 and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), HLA-A (HLAA) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    

    Tags

    nuclease A from S. aureus fused to the poly-Leu T…

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and predic…

    Promoter

    T7 promoter

    Available Since

    Jan. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSN-A112C-CP8B1

    Plasmid

    #191240

    Purpose

    Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of poly-Leu-CP8B1 and a His-tag, for fluorescent studies.

    Depositor

    Alessandro Senes

    Article

    Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

    Insert

    nuc (nuclease A), CYP8B1 (CYP12) (predicted interfacial residues of the transmembrane domain in a poly-leu background)
    

    Tags

    nuclease A from S. aureus fused to the poly-Leu T…

    Expression

    Bacterial

    Mutation

    Changed Alanine 112 to Cysteine in SN, and predic…

    Promoter

    T7 promoter

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTWIN1-His6-Ssp-Htt2-90(A2C)-49Q-P70C

    Plasmid

    #114629

    Purpose

    Bacterial expression of Htt fragment containing amino acids 2-90, with A2C & P70C cysteine mutations and a 49 polyQ repeat

    Depositor

    Hilal Lashuel

    Article

    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456

    Insert

    Huntingtin Exon1 fragment (HTT Human)
    

    Tags

    His6-Ssp DnaB intein

    Expression

    Bacterial

    Mutation

    A2C, P70C; 49 polyQ repeat

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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