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pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B

Plasmid

#223712

Purpose

Expresses autophagy flux LC3B fluorescence reporter

Depositor

Benjamin Wolozin

Publication

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

LC3B (MAP1LC3B Human, Synthetic)

Use

Lentiviral and Synthetic Biology

Tags

mCherry::EGFP::LC3B

Expression

Mammalian

Promoter

CMV

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pFA6a-SNAP-KanMX6

Plasmid

#87024

Purpose

Tag S. pombe genes in C terminal with SNAP

Depositor

Julien Berro

Publication

Lacy et al Mol Biol Cell. 2017 Jun 28. pii: mbc.E17

Insert

SNAP

Expression

Bacterial and Yeast

Available since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pcDNA4-FLAG-Jhdm2a-H1122A

Plasmid

#38140

Depositor

Toshinobu Nakamura, Toru Nakano

Publication

Nakamura et al Nature. 2012 Jun 3;486(7403):415-9. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Jhdm2a-H1122A (Kdm3a Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

protein starts at aa115; H1122A

Promoter

CMV

Available since

Sept. 28, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLKO.1-TetON-Puro-HIF1a 0819

Plasmid

#118704

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Publication

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Available since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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EasyFusion T2A-H2B-miRFP703

Plasmid

#113097

Purpose

General donor vector with T2A-H2B-miRFP703 coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

T2A-H2B-miRFP703

Use

Synthetic Biology

Available since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMaCTag-P23

Plasmid

#120034

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Halo

Depositor

Michael Knop

Publication

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

Halo

Use

Pcr template

Tags

Halo

Promoter

None

Available since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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AcrIIA4

Plasmid

#101043

Purpose

Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

Depositor

Zhiwei Huang

Publication

Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

Insert

AcrIIA4

Tags

GST

Expression

Bacterial

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pSMALB-ATF4.5

Plasmid

#155032

Purpose

Fluorescent reporter for ATF4 translation

Depositor

John Dick, Peter van Galen

Publication

van Galen et al Nature. 2014 Jun 12;510(7504):268-72. do

Inserts

ATF4 5' UTR (Atf4 Mouse)
ATF4-eGFP fusion ORF
TagBFP

Use

Lentiviral

Expression

Mammalian

Available since

Aug. 19, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GFP shRNA tet pLKO puro

Plasmid

#163017

Purpose

Control tet-inducible shRNA targeting eGFP

Depositor

Mark Rubin

Publication

Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

Insert

Enhanced Green Fluorescent Protein

Use

Lentiviral

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTOX1

Plasmid

#127446

Purpose

Encodes YhaV toxin. CAM-R

Depositor

Matthew Waldor

Publication

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

yhaV toxin

Expression

Bacterial

Promoter

pRha

Available since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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PBS Actb-2A-TIR1-GFP

Plasmid

#113832

Purpose

targeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

osTIR1-GFP (NEWENTRY Synthetic)

Use

Synthetic Biology

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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EasyFusion eGFP

Plasmid

#112848

Purpose

General donor vector with EGFP coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EGFP

Use

Unspecified; General donor

Promoter

No

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pX330A-1x5

Plasmid

#58769

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pX330A-1x7

Plasmid

#58771

Purpose

Expresses Cas9 nuclease and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

humanized S. pyogenes Cas9 nuclease

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pX330A_D10A-1x4

Plasmid

#58774

Purpose

Expresses Cas9 nickase and gRNA

Depositor

Takashi Yamamoto

Publication

Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

Insert

humanized S. pyogenes Cas9 (D10A) nickase

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

D10A nickase-converting mutation

Promoter

CBh

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRK5-myc-Miro1 K427V

Plasmid

#47892

Purpose

Expresses myc tagged Miro1 K427 constitutively active mutant

Depositor

Pontus Aspenström

Publication

Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Miro1 K427V (RHOT1 Human)

Tags

myc

Expression

Mammalian

Mutation

K427V, constitutively active mutant

Promoter

CMV

Available since

Sept. 12, 2013

Availability

Academic Institutions and Nonprofits only
pBp-FGFR3c-S249C

Plasmid

#45713

Depositor

Matthew Meyerson

Publication

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR3 IIIc (FGFR3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

S249C

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
pBITE-EGFRvIII-F269-OKT3

Plasmid

#190681

Purpose

Encodes an EGFRvIII-CD3 BITE protein (F269 clone)

Depositor

Scott McComb

Publication

Shepherd et al PLoS One. 2023 Jun 22;18(6):e0273884. do

Insert

Anti-EGFRvIII-scFv-F269-modularized-OKT3-HisTag

Use

Lentiviral

Tags

mNeonGreen

Expression

Mammalian

Promoter

EFS

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAmCyan1-C1-ADCY6

Plasmid

#113905

Purpose

N-terminal Cyan Fluorescent tag in Adenylate Cyclase 6

Depositor

Anjaparavanda Naren

Publication

Thomas et al J Biol Chem. 2018 Jun 14. pii: RA118.003

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Adenylate Cyclase 6 (ADCY6 Human)

Tags

Cyan

Expression

Bacterial and Mammalian

Promoter

pCMV

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pHSS6hsILMi20

Plasmid

#102536

Purpose

Helper plasmid expressing Minos transposase mRNA (Drosophila hsp70 promoter)

Depositor

Michalis Averof, Charalambos Savakis

Publication

Klinakis et al Insect Mol Biol. 2000 Jun;9(3):269-75.

Insert

Minos transposase

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

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