We narrowed to 5,078 results for: mcs

1. px552-sg-gria1-HT-SEP

   Plasmid

   #187653

   Purpose

   Contains HaloTag-SEP to be inserted into the NTD of Gria1 (via HITI) and single guide RNA to target Cas9 to Gria1 under control of the U6 promoter.

   Depositor

   Erin O'Shea

   Article

   Wong et al Elife. 2024 Aug 15;13:e80622. doi: 10.75

   Insert

   HaloTag-SEP donor
   

   Use

   AAV and CRISPR

   Tags

   N/A

   Promoter

   N/A

   Available Since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. (326) pAAV SV40-ZsGreen U6-gRNA

   Plasmid

   #163020

   Purpose

   Fluorescent reporter for Sa gRNA (Bsa1 sites)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Zs Green
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. isoform PKC lambda (JCE590)

   Plasmid

   #89775

   Purpose

   yeast expression of mouse PKC lambda with yeast PKC1 promoter and ADH1 terminator

   Depositor

   Juan Carlos Igual

   Article

   Soriano-Carot et al Nucleic Acids Res. 2014 Jun;42(11):7084-

   Insert

   PKC lambda (Prkci Mouse)
   

   Expression

   Mammalian

   Promoter

   yeast PKC1 promoter (-600 to -1)

   Available Since

   May 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-TRIM28deltaCC1

   Plasmid

   #124955

   Purpose

   Mammalian expression of Human TRIM28 with deleted CC domain

   Depositor

   Michelle Barton

   Article

   Li et al Oncogene. 2017 May 25;36(21):2991-3001.

   Insert

   hTRIM28 (TRIM28 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deletion of CC domains

   Available Since

   May 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSEM390 - mosTI unc-119 - Peft-3 - NNN - tbb-2

   Plasmid

   #182350

   Purpose

   Ubiquitous expression cloning vector for mosTI(unc-119). Transgene inserted between Peft-3 and tbb-2 3'UTR. MCS or Golden Gate (BsaI: aaaa - MCS - tgag)

   Depositor

   Christian Froekjaer-Jensen

   Article

   El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. 3540 pMIG Bid p15

   Plasmid

   #8789

   Depositor

   Stanley Korsmeyer

   Article

   Cheng et al Mol Cell 2001 Sep;8(3):705-11.

   Insert

   Bid p15 (Bid Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   July 6, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pCOLA kiwi sensor sfGFP

   Plasmid

   #107368

   Purpose

   Expresses sfGFP (a GFP) in Ecoli off a T7 promoter when exposed to extracted and RPA-amplified kiwi DNA

   Depositor

   James Collins

   Article

   Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

   Insert

   kiwi-detecting toehold + sfGFP
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. 3538 pMIG Bad

   Plasmid

   #8787

   Depositor

   Stanley Korsmeyer

   Article

   Cheng et al Mol Cell 2001 Sep;8(3):705-11.

   Insert

   Bad (Bad Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 19, 2005

   Availability

   Academic Institutions and Nonprofits only

9. pLenti-lxlplap

   Plasmid

   #45581

   Depositor

   Nirao Shah

   Article

   Yang et al Cell. 2013 May 9;153(4):896-909. doi: 10

   Inserts

   Green fluorescent protein
   Placental alkaline phosphatase (ALPP Human)
   

   Use

   Lentiviral

   Tags

   Histone 2B

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR SD Age HsSAS-6

    Plasmid

    #46381

    Purpose

    For expression of C-terminal fusions.

    Depositor

    Pierre Gonczy

    Article

    Kitagawa et al Cell. 2011 Feb 4;144(3):364-75. doi: 10.

    Insert

    SAS-6 (SASS6 Human)
    

    Use

    Entry vector

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGEX-EGFAB(H306Y)

    Plasmid

    #25771

    Depositor

    Johann Deisenhofer

    Article

    McNutt et al J Biol Chem. 2009 Apr 17. 284(16):10561-

    Insert

    LDL receptor EGFAB (LDLR Human)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    His 306 to Tyr

    Available Since

    Dec. 3, 2010

    Availability

    Academic Institutions and Nonprofits only

12. S12.1_Empty_Bridge

    Plasmid

    #127670

    Purpose

    Ampicillin resistant multiplexing backbone without CRY2HA and mCherry

    Depositor

    Jennifer Phillips-Cremins

    Article

    Kim et al Nat Methods. 2019 Jun 24. pii: 10.1038/s

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. AIRAP 10: PSMD14.TEV.GST.V1.pCDNA3

    Plasmid

    #21800

    Depositor

    David Ron

    Article

    Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

    Insert

    PSMD14 (Psmd14 Mouse)
    

    Tags

    GST and TEV

    Expression

    Mammalian

    Available Since

    Sept. 22, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCLR2070

    Plasmid

    #28063

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains N496D point mutation in FokI domain, as …

    Available Since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCLR2068

    Plasmid

    #28062

    Depositor

    Keith Joung

    Article

    Miller et al Nat Biotechnol. 2007 Jul . 25(7):778-85.

    Insert

    FokI endonuclease
    

    Use

    Zebrafish expression

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains H537R point mutation in FokI domain, as …

    Available Since

    July 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pV2A-T

    Plasmid

    #153022

    Purpose

    Single-plasmid multi-gene expression system suitable for fungi. The obtained polycistronic gene is expressed under the control of a TetON inducible promoter

    Depositor

    Vito Valiante

    Article

    Hoefgen et al Metab Eng. 2018 Jul;48:44-51. doi: 10.10

    Type

    Empty backbone

    Expression

    Bacterial and Yeast

    Available Since

    Oct. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSEM398 - mosTI unc-119 - NNN - operon - mScarlet - tbb-2

    Plasmid

    #182355

    Purpose

    mScarlet co-expression cloning vector for mosTI(unc-119). Transgene inserted upstream of gpd-2 operon::mScarlet::tbb-2 3'UTR. MCS or Golden Gate (BsaI: tgcc - MCS - tgag)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pJL1 Trx-Bx

    Plasmid

    #106290

    Purpose

    Expresses Trx-Bx in Ecoli off a T7 promoter

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    Trx-BX
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV Aquamarine-superYFP tandem

    Plasmid

    #157776

    Purpose

    Produces a fusion between Aquamarine and superYFP. It can be used as a positive control for FRET (using FLIM)

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Type

    Empty backbone

    Tags

    Aquamarine and superYFP

    Expression

    Mammalian

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSEM387 - mosTI unc-119 - NNN - gfp - nls - tbb-2

    Plasmid

    #182347

    Purpose

    GFP transcriptional reporter cloning vector for mosTI(unc-119). Transgene inserted upstream of gfp(2xNLS) and tbb-2 3' UTR. MCS or Golden Gate (BsaI: tgcc - MCS - aaaa)

    Depositor

    Christian Froekjaer-Jensen

    Article

    El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

    Type

    Empty backbone

    Expression

    Worm

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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