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Plasmid#154824Purpose(eft-3p::wrmScarlet::tbb-2 3'UTR in pUC19) Experimentally used to mark formation of transgenic arrays for C. elegansDepositorInsertPeft-3::wrmScarlet::tbb-2 UTR
ExpressionWormPromotereef-1A.1 (eft-3)Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
NYESO alpha/beta into TRBC1 HDRT Source (pTR 262)
Plasmid#112022PurposeDNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-betaDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNYESO alpha/beta into TRBC1 HDRDT
UseCRISPR and Synthetic BiologyAvailable sinceNov. 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
β₂AR-mCFP
Plasmid#38260DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertβ₂AR (ADRB2 Human)
TagsFlag, mCFP, and signal peptide sequence (MKTIIALS…ExpressionMammalianMutationdiglycine linker (replacing terminator codon)PromoterCMVAvailable sinceAug. 23, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pgLAP5
Plasmid#19706DepositorInsertpgLAP5
TagsEGFP, Precission Protease site, and S peptideExpressionMammalianAvailable sinceFeb. 19, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PEmax-mRNA
Plasmid#204472Purposeplasmid for PEmax mRNA IVTDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPrime editor
UseCRISPRTagsnoExpressionMammalianPromoterT7Available sinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCWori cytochrome P450-BM3h 9D7
Plasmid#61308PurposeExpresses Cytochrome P450-BM3h 9D7 with C-terminal 6-His tagDepositorInsertCytochrome P450-BM3 heme domain 9D7
Tags6xHisExpressionBacterialMutationT268A, I263A, T438V, A328GPromoterTacAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-CMV-MCS-Puro-EGFP
Plasmid#219795PurposeMammalian expression plasmids using piggyBac Transposon SystemDepositorTypeEmpty backboneUsePiggybac transposonExpressionMammalianAvailable sinceAug. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-mTagBFP2
Plasmid#96935PurposeRetroviral plasmid containing mTagBFP2 for labeling of cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMonomeric Tag Blue Fluorescent Protein
ExpressionMammalianAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mEGFP-H4-23
Plasmid#56463PurposeLocalization: Nucleus/Histones, Excitation: 488, Emission: 507DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH4 (HIST1H4J Human)
TagsmEGFPExpressionMammalianPromoterCMVAvailable sinceDec. 23, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
tdTomato-TOMM20-N-10
Plasmid#58137PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 554, Emission: 581DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertTOMM20 (TOMM20 Human)
TagstdTomatoExpressionMammalianPromoterCMVAvailable sinceDec. 18, 2014AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-MyoAAV4A
Plasmid#224448PurposeRep/Cap plasmid for the production of MyoAAV 4A, a muscle-tropic AAV capsid in mice and macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationRGDYNSL insert between amino acids 588 and 589 of…Promoterp41Available sinceSept. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-LaminA-C-18
Plasmid#54138PurposeLocalization: Nuclear Envelope, Excitation: 487, Emission: 509DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLaminA (LMNA Human)
TagsmEmeraldExpressionMammalianPromoterCMVAvailable sinceJuly 10, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pDNA2.0 6H-FLAG-TEV-KRAS G12C
Plasmid#111848PurposeExpresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.DepositorInsertKRAS G12C (KRAS Human)
Tags6xHis-tag, FLAG-tag, and TEV protease cleavage se…ExpressionBacterialMutationChanged Glycine 12 to CysteinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSU-araC-Cas9
Plasmid#120423PurposeMultigene editing in the Escherichia coli genome using the CRISPR-Cas9 systemDepositorInsertCas9
UseCRISPRExpressionBacterialPromoteraraBADAvailable sinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
retro-gRNA-eGFP
Plasmid#116926PurposeRetrovirus expressing eGFP with BBSI cloning sites for sgRNADepositorInserteGFP
UseRetroviralPromoterEF1aAvailable sinceOct. 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pH-nCas9-PPE
Plasmid#140447PurposeFor plant prime editing in rice plants or monocotyledons protoplastsDepositorInsertnCas9(H840A)-M-MLV
UseCRISPRExpressionPlantMutationH840A for Cas9; D200N, T306K, W313F, T330P and L…Promotermaize Ubiquitin-1, OsU3Available sinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS
Plasmid#89508PurposeExpression of Hisx6-rAPOBEC1-XTEN-nCas9-UGI-NLS in bacterial cells.DepositorInsertrAPOBEC1-nCas9 (D10A)-UGI-NLS
TagsUGI and rAPOBEC-XTENExpressionBacterialMutationD10A mutation of Cas9PromoterT7Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBAD-bARGSer-AxeTxe
Plasmid#192473PurposeSecond-generation bacterial acoustic reporter gene derived from SerratiaDepositorInsertsbARGSer
AxeTxe
ExpressionBacterialMutationthe gene Ser39006_001280 was deleted from the clu…PromoterNative promoter from Enterococcus faecium and pBADAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGTag-gal4-VP16-B-actin
Plasmid#117817PurposeDonor for precise CRISPR directed genomic integration using the GeneWeld methodDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgal4/VP16
UseSynthetic BiologyAvailable sinceMarch 15, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB CAG-tdTomato
Plasmid#133569PurposeCAG tdTomato piggyBac transposonDepositorInserttdTomato
ExpressionMammalianAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits onlyCited by