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pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

Plasmid

#181975

Purpose

Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

Insert

FMC6.3-BBz CAR; tNGFR (NGFR Human, Synthetic)

Use

Lentiviral

Mutation

NGFR truncation: aa 11-276

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLY094-EFS-CD22-Blast-WPRE

Plasmid

#192195

Purpose

Lenti-CD22-OE-Blast

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-CD22-OE-Blast (CD22 Synthetic)

Use

Lentiviral; Mammalian expression

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pLY096-RetroEFS-CD22BBz-WPRE

Plasmid

#192197

Purpose

Retro-CD22CAR

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-CD22CAR (CD22 Synthetic)

Use

Retroviral; Mammalian expression

Mutation

NA

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pICOZ-SB100X[EF1α-CD19 4-1BB-eGFP]

Plasmid

#218343

Purpose

Donor plasmid of SB100X Transposon with CD19 CAR mammalian expression cassette

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

CD19 CAR mammalian expression cassette

Use

Lentiviral

Expression

Mammalian

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pLY097-RetroEFS-CD22BBz-PRODH2-WPRE

Plasmid

#192198

Purpose

Retro-CD22CAR-PRODH2

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-CD22CAR-PRODH2 (CD22 Synthetic)

Use

Retroviral; Mammalian expression

Mutation

NA

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLY100-RetroEFS-HER2-28BBz-WPRE

Plasmid

#192201

Purpose

Retro-HER2CAR

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-HER2CAR (ERBB2 Synthetic)

Use

Retroviral; Mammalian expression

Mutation

NA

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLY101-RetroEFS-HER2-28BBz-PRODH2-WPRE

Plasmid

#192202

Purpose

Retro-HER2CAR-PRODH2

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Retro-HER2CAR-PRODH2 (ERBB2 Synthetic)

Use

Retroviral; Mammalian expression

Mutation

NA

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLY095-EFS-ERBB2 HER2-Blast-WPRE

Plasmid

#192196

Purpose

Lenti-HER2-OE-Blast

Depositor

Sidi Chen

Article

Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

Insert

Lenti-HER2-OE-Blast (ERBB2 Synthetic)

Use

Lentiviral; Mammalian expression

Mutation

NA

Available Since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

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Human Epigenetic Knockout Library

Pooled Library

#162256

Purpose

Knockout library that targets human epigenetic genes.

Depositor

Kivanc Birsoy

Article

Williams et al Cell Metab. 2020 Apr 7;31(4):852-861.e6.

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only
R-G324D-(Kin-8)

Plasmid

#28179

Depositor

Bernard Schimmer

Article

Olson et al Mol Endocrinol. 1993 Apr . 7(4):477-87.

Insert

Prkar1a (PRKAR1A Mouse)

Expression

Mammalian

Mutation

G324D

Available Since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
R-R334W-(Kin-7)

Plasmid

#28178

Depositor

Bernard Schimmer

Article

Olson et al Mol Endocrinol. 1993 Apr . 7(4):477-87.

Insert

Prkar1a (PRKAR1A Mouse)

Expression

Mammalian

Mutation

R334W

Available Since

May 12, 2011

Availability

Academic Institutions and Nonprofits only
pMN-285

Plasmid

#195472

Purpose

Lentiviral Integratable Inducible CD19CAR using synZiFTR ZF10

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

8X ZF10 BS - ybTATA - CD19 CAR - mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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Lourido Toxoplasma CRISPR Library V1

Pooled Library

#80636

Purpose

Knockout library targeting 8,158 predicted protein-coding gene in Toxoplasma gondii.

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Available Since

Sept. 19, 2016

Availability

Academic Institutions and Nonprofits only
Compact mismatched sgRNA library for gene titration (human essential genes, CRISPRi)

Pooled Library

#136479

Purpose

Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi

Depositor

Jonathan Weissman

Article

Jost et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only
Large-scale mismatched sgRNA library for gene titration (human essential genes, CRISPRi)

Pooled Library

#136478

Purpose

Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi

Depositor

Jonathan Weissman

Article

Jost et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

Expression

Mammalian

Species

Homo sapiens

Use

CRISPR and Lentiviral

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only
Mouse Chromatin Regulator Library

Pooled Library

#200011

Purpose

Knockout library targeting a comprehensive set of mouse genes with annotated function as chromatin regulators.

Depositor

Bradley Bernstein, Gabriel Griffin, Robert Manguso

Article

Griffin et al Nature. 2021 Jul;595(7866):309-314. doi:

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only
EGFP miSFIT Lineage Barcoding Library

Pooled Library

#229136

Purpose

The EGFP_miSFIT_Lineage_BC_lib library is a transcribed lineage barcoding vector library.

Depositor

Peter Zandstra

Article

Michaels et al Cell Syst. 2024 Dec 18;15(12):1245-1263.

Expression

Mammalian

Use

Lentiviral

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only
plIG-106_NY-ESO-1_TCR_SR_Library

Pooled Library

#207474

Purpose

NY-ESO-1 TCR in combination with 129 surface receptors and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the TRAC locus of human T cells

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
plIG-107_NY-ESO-1_TCR_TF_Library

Pooled Library

#207475

Purpose

NY-ESO-1 TCR in combination with 100 transcription factors (and related proteins) and 2 controls (GFP/RFP). Library can be used to generate HDR templates for modular pooled knockin (ModPoKI) into the

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Species

Homo sapiens

Use

CRISPR

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only
SBC015866

Plasmid

#226280

Purpose

Expresses BsSfp, MsCAD, and SrCAR from trc promoter. Biosynthesis of (hydroxy)cinnamyl alcohols from (hydroxy)cinnamic acids.

Depositor

Rainer Breitling

Article

Hanko et al Microb Cell Fact. 2023 Nov 18;22(1):238.

Inserts

4'-phosphopantetheinyl transferase Sfp
Cinnamyl alcohol dehydrogenase
Carboxylic acid reductase

Expression

Bacterial

Available Since

Nov. 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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