We narrowed to 54,068 results for: tra

1. pBluescript-Evf1

   Plasmid

   #99479

   Purpose

   Subcloning of the Evf1 long non-coding RNA

   Depositor

   Jhumku Kohtz

   Article

   Feng et al Genes Dev. 2006 Jun 1;20(11):1470-84. Ep

   Insert

   Evf1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLfa-PUb-EGFP-3'UTR#5

   Plasmid

   #176337

   Purpose

   The synthetic 3'UTR#5 for EGFP expression under the control of the Ae. aegypti polyubiquitin (PUb) promoter

   Depositor

   Zach Adelman

   Article

   Chae et al RNA Biol. 2021 Aug 31:1-9. doi: 10.1080/

   Insert

   3'UTR#5
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. dSaCas9-KRAB

   Plasmid

   #205250

   Purpose

   In vitro transcription plasmid for production of dSaCas9-KOX1 KRAB mRNA for transcriptional activation (KRAB = Krüppel-associated box). Insert is derived from Addgene plasmid #106219.

   Depositor

   Rasmus Bak

   Insert

   dSaCas9-KRAB
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. NAT-TET-OFF -MET - 3xHA

   Plasmid

   #182526

   Purpose

   Doxycycline-induced transcriptional regulation and 3xHA N-terminal tagging.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   7 Copies of TetO Box
   

   Use

   Tags

   3HA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTYF-1xSYN-tTA

   Plasmid

   #19980

   Depositor

   Sergey Kasparov

   Article

   Liu et al J Gene Med. 2008 May . 10(5):583-92.

   Insert

   SYN-tTA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET26-CNTnw

   Plasmid

   #100163

   Purpose

   recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

   Depositor

   Seok-Yong Lee

   Article

   Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

   Insert

   Concentrative nucleoside transporter
   

   Use

   Tags

   MBP-His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. SG-3'REL

   Plasmid

   #27257

   Depositor

   Thomas Gilmore

   Article

   Starczynowski et al Oncogene. 2003 Oct 9. 22(44):6928-36.

   Insert

   REL (REL Human)
   

   Use

   Tags

   GAL4

   Expression

   Mammalian

   Mutation

   Contains aa 278-587 of REL

   Promoter

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pET26-CNTN149L

   Plasmid

   #100164

   Purpose

   recombinant expression of target protein as an MBP-His tag fusion at the N-terminus of target protein

   Depositor

   Seok-Yong Lee

   Article

   Hirschi et al Nature. 2017 May 4;545(7652):66-70. doi:

   Insert

   Concentrative nucleoside transporter
   

   Use

   Tags

   MBP-His

   Expression

   Bacterial

   Mutation

   N149L

   Promoter

   T7

   Available since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC-TalO8 (Blank-CoTrIP)

   Plasmid

   #178303

   Purpose

   Blank CoTrIP plasmid

   Depositor

   Brian Zid

   Article

   Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

   Type

   Empty backbone

   Use

   Tags

   NA

   Expression

   Yeast

   Mutation

   Promoter

   NA

   Available since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRL623

    Plasmid

    #58494

    Purpose

    helper plasmid for bacterial conjugal DNA transfer

    Depositor

    Peter Wolk

    Article

    Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

    Insert

    encodes methylases AvaiM, Eco47iiM, and Ecot22iM that target sites subject to restriction in Anabaena sp. strain PCC 7120
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCL28-decoder

    Plasmid

    #159450

    Purpose

    Insert: PlexAtet-lexA-ER-B112-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is self-inducible upon addition of estradiol, and repressible by TetR. Backbone: pRG206MX. Marker: Ura3.

    Depositor

    Joerg Stelling

    Article

    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

    Insert

    PlexAtet-LexA-ER-B112-tCyc1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PlexAtet

    Available since

    Dec. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSOUP

    Plasmid

    #165419

    Purpose

    The pSOUP helper plasmid needed for replication of all pGreen (Hellens et al., 2000) based vectors in Agrobacterium

    Depositor

    John Innes Centre - Wheat Transformation and Genome Editing, Wendy Harwood

    Article

    Hayta et al Curr Protoc. 2021 Mar;1(3):e58. doi: 10.

    Insert

    Rep A
    

    Use

    Helper plasmid required for replication for all p…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCMV-BI

    Plasmid

    #126475

    Purpose

    High-copy number vector for the co-expression of two genes in mammalian cells

    Depositor

    Bill Hansson

    Article

    Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRL278

    Plasmid

    #70256

    Purpose

    sacB plasmid with polylinker; kanamycin/neomycin resistance: used as a conditionally lethal gene in Anabaena sp. strain PCC 7120 to select for double recombinants and to entrap insertion seq.

    Depositor

    Peter Wolk

    Article

    Cai et al J Bacteriol. 1990 Jun;172(6):3138-45.

    Type

    Empty backbone

    Use

    Conditionally lethal

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3-TfR-OVA

    Plasmid

    #64600

    Purpose

    Expression of chicken ovalbumin fused to the transmembrane domain of transferrin receptor as a model antigen for study of MHC II presentation

    Depositor

    Sandra Diebold, Martin Zenke

    Article

    Diebold et al Gene Ther. 2001 Mar;8(6):487-93.

    Insert

    Ovalbumin
    

    Use

    Tags

    Transmembrane domain of Transferrin Receptor (TfR…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. Zic4-3505:GFP

    Plasmid

    #193001

    Purpose

    Plasmid to generate a transgenic Hydra Zic4 reporter line

    Depositor

    Brigitte Galliot

    Article

    Vogg et al bioRxiv 2021.12.22.47383

    Insert

    Hydra Zic4 promoter
    

    Use

    Hydra transgenesis vector

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET28a-Ec.coaD (pESC106)

    Plasmid

    #50388

    Purpose

    Heterologous expression of E. coli CoA biosynthetic enzyme, PPAT (phosphopantetheine adenylyltransferase). Contain 6xHis-tag for nickel affinity purification.

    Depositor

    Tadhg Begley, Erick Strauss

    Article

    Strauss et al J Biol Chem. 2002 Dec 13;277(50):48205-9

    Insert

    PPAT
    

    Use

    Tags

    6xHis-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. mNeonGreen-Sec31A

    Plasmid

    #226582

    Purpose

    Encodes Sec31A protein labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Westrate et al J Cell Sci. 2020 Jul 24;133(14). pii: jc

    Insert

    Sec31A (Protein transport protein) (SEC31A Human)
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. ptRNAfMet-A37

    Plasmid

    #196657

    Purpose

    Plasmid for overexpression of native initiator tRNA (A37)

    Depositor

    Markus Jeschek

    Article

    Hollerer et al Nucleic Acids Res. 2023 Mar 21;51(5):237

    Insert

    tRNAfMet-A37 expression cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    metY native promoter

    Available since

    March 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTns(Ptr)

    Plasmid

    #175576

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

    Insert

    PtrTnsABC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    NO

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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