We narrowed to 4,259 results for: Lta

1. pXNubWtgate

   Plasmid

   #105080

   Purpose

   Yeast mating-based Split Ubiquitin Assay, positive control

   Depositor

   Wolf Frommer

   Article

   Obrdlik et al Proc Natl Acad Sci U S A. 2004 Aug 17;10

   Type

   Empty backbone

   Use

   Tags

   NubI

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMA-SpCas9-g10

   Plasmid

   #80793

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 10/20/30 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pMA-SpCas9-g9

   Plasmid

   #80792

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 9/19/29 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMA-SpCas9-g8

   Plasmid

   #80791

   Purpose

   BsaI-based cloning of SpCas9 gRNA guide sequence, position 8/18/28 in the array

   Depositor

   Yonglun Luo

   Article

   Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

   Insert

   CRISPR gRNA expression cassette (for SpCas9)
   

   Use

   CRISPR

   Tags

   na

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMS8: pTns(AvCAST)

   Plasmid

   #168141

   Purpose

   Inducible expression of AvCAST TnsA, TnsB, TnsC, TniQ and TnsD proteins.

   Depositor

   Feng Zhang

   Article

   Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

   Inserts

   AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
   AvTnsD
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAWH

   Plasmid

   #133894

   Purpose

   Expression of Gal4AD-Prey hybrid protein. Homology regions for recombination with pBKWH, pBSWHhc or pMS22H

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBKWH

   Plasmid

   #133898

   Purpose

   Expression of Gal4BD-Bait hybrid protein. Homology regions for recombination with pAWH

   Depositor

   Sebastian Maurer

   Article

   Yang et al Nat Commun. 2018 Sep 14;9(1):3747. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMVS-V1

   Plasmid

   #183094

   Purpose

   Basic conjugative shuttle vector for Escherichia coli and Methanothermobacter thermautotrophicus

   Depositor

   Lars Angenent, Bastian Molitor

   Article

   Fink et al mBio. 2021 Dec 21;12(6):e0276621. doi: 1

   Inserts

   pME2001
   thermostable neomycin phosphotransferase gene
   

   Use

   Bacterial propagation and conjugative

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pOTTC1553 - pAAV EF1a EGFP-KASH

   Plasmid

   #131682

   Purpose

   An adeno-associated viral vector expressing nuclear envelope-embedded eGFP

   Depositor

   Christopher Richie

   Article

   Gantz et al Elife. 2020 Apr 1;9. pii: 56054. doi: 10

   Insert

   EGFP-KASH
   

   Use

   AAV

   Tags

   KASH

   Expression

   Mutation

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMS15: pTwinStrep-SUMO-AvTnsB

    Plasmid

    #168148

    Purpose

    AvTnsB expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    AvCAST TnsB
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMS16: pTwinStrep-SUMO-AvTnsC

    Plasmid

    #168149

    Purpose

    AvTnsC expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    AvCAST TnsC
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMS14: pTwinStrep-SUMO-AvTnsA

    Plasmid

    #168147

    Purpose

    AvTnsA expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    AvCAST TnsA
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pMS32: pTwinStrep-SUMO-PmcTnsC

    Plasmid

    #168165

    Purpose

    PmcTnsC expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    PmcCAST TnsC
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRN3P_T3_BE3_IVT

    Plasmid

    #177015

    Purpose

    Plasmid to be used as DNA template for in-vitro RNA transcription of the BE3 base editor (C to T) by T3 RNA polymerase

    Depositor

    Timo Otonkoski

    Article

    Jalil et al Stem Cell Reports. 2021 Nov 16. pii: S22

    Insert

    BE3
    

    Use

    CRISPR; Vector for in-vitro transcription

    Tags

    Expression

    Mutation

    Promoter

    T3 promoter

    Available since

    Dec. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA-NES-ZapCmR2

    Plasmid

    #59015

    Purpose

    Cytosolic zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NES-ZapCmR2 genetically encoded cytosolic Zn(II) sensor
    

    Use

    Tags

    Nuclear Exclusion Signal

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA-NLS-ZapCmR2

    Plasmid

    #59012

    Purpose

    Nuclear zinc-binding FRET biosensor

    Depositor

    Amy Palmer

    Article

    Miranda et al PLoS One. 2012;7(11):e49371. doi: 10.137

    Insert

    NLS-ZapCmR2 genetically encoded Zn(II) sensor
    

    Use

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    See comment

    Promoter

    CMV

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

17. NavB4-GFP/pEGFP-N1

    Plasmid

    #197310

    Purpose

    Plasmid expressing an antigen targeting the Nav beta4 sodium channel subunit

    Depositor

    Lori Isom

    Article

    Aman et al J Neurosci. 2009 Feb 18;29(7):2027-42. d

    Insert

    NavB4-GFP (Scn4b Rat)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. BK beta1

    Plasmid

    #113565

    Purpose

    mouse BK beta1 subunit in mammalian expression vector

    Depositor

    Robert Brenner

    Article

    Wang et al J Gen Physiol. 2006 Dec;128(6):731-44. d

    Insert

    Kcnmb1 (Kcnmb1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMS31: pTwinStrep-SUMO-PmcTnsAB

    Plasmid

    #168164

    Purpose

    PmcTnsAB expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    PmcCAST TnsAB
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1-scrCdh2.1 GFP

    Plasmid

    #209100

    Purpose

    GFP expressing scramble of shRNA targeting Cdh2

    Depositor

    Cagla Eroglu

    Article

    Tan et al J Cell Biol. 2023 Nov 6;222(11):e2023031

    Insert

    scrCdh2.1
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Plas-gRNA-LEU2

    Plasmid

    #157656

    Purpose

    GFP and gRNA tag for protein and mRNA quantification, to attache to the 3' end of the gene coding sequence

    Depositor

    Frank Albert

    Article

    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

    Insert

    GFP-gRNA
    

    Use

    Insert storage (replicative in e. coli)

    Tags

    GFP-gRNA

    Expression

    Mutation

    Promoter

    Available since

    Sept. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. MPC020: CamKII CMV_NovArch_citrine

    Plasmid

    #153193

    Purpose

    Encodes Novarch

    Depositor

    Adam Cohen

    Article

    Chien et al Sci Adv. 2021 May 5;7(19). pii: 7/19/eab

    Insert

    NovArch
    

    Use

    Tags

    citrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSV(X) C/EBPbeta

    Plasmid

    #13393

    Depositor

    Richard Schwartz

    Article

    Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

    Insert

    C/EBPbeta (Cebpb Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

24. pCLC-mEos2

    Plasmid

    #38010

    Depositor

    Xiaowei Zhuang

    Article

    Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

    Insert

    Clathrin, light polypeptide (LCa) (Clta Mouse)
    

    Use

    Tags

    mEos2

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMS33: pTwinStrep-SUMO-PmcTnsD

    Plasmid

    #168166

    Purpose

    PmcTnsD expression construct for protein purification.

    Depositor

    Feng Zhang

    Article

    Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

    Insert

    PmcCAST TnsD
    

    Use

    Tags

    6X His, Twin Strep and SUMO

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSV(X) C/EBPalpha

    Plasmid

    #13392

    Depositor

    Richard Schwartz

    Article

    Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

    Insert

    C/EBPalpha (Cebpa Rat)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

27. pPPC017.109

    Plasmid

    #171145

    Purpose

    Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    J109 scRNA
    J1-BBa_J23117-mRFP
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119 and J1-BBa_J23117

    Available since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. PX552-mScn2a-HaloTag-V5-EF1-sfGFP

    Plasmid

    #182564

    Purpose

    For mouse Nav1.2 knockin with HaloTag-V5 at the C-terminal

    Depositor

    James Zhe Liu

    Article

    Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

    Inserts

    mouse Scn2a gRNA and HaloTag-V5 donor DNA
    sfGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1 and U6

    Available since

    March 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCG-D-mSpyCatcher-His6-E

    Plasmid

    #87376

    Purpose

    An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with a His6 tag at its C-terminus and 4 bp overhangs specific for the ‘downstream SpyPart’ position within an ORF ass

    Depositor

    Tom Ellis

    Article

    Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

    Insert

    mSpyCatcher
    

    Use

    Synthetic Biology

    Tags

    His6 tag

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pTRCHisB-TRF2∆B

    Plasmid

    #53208

    Purpose

    Expressed N-terminally Hexahistine tagged TRF2 Delta Basic protein

    Depositor

    Jack Griffith

    Article

    Bower et al Biochemistry. 2014 Sep 2;53(34):5485-95.

    Insert

    TRF2 Delta Basic (TERF2 Human)
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    contains amino acids 47-500

    Promoter

    Trc

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge