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We narrowed to 5,900 results for: pcas

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Showing: 1661 - 1680 of 5900 results

  1. pCAG-T7-TALEN(Sangamo)-Destination

    Plasmid
    #37184
    Purpose
    Destination vector for Voytas Golden Gate TALEN assembly incorporating a CAG promoter for mammalian expression and a T7 promoter for synthesis of TALEN mRNA. Uses homodimeric FokI domains.
    Depositor
    Pawel Pelczar
    Article
    Hermann et al J Vis Exp. 2014 Apr 2;(86). doi: 10.3791
    Type
    Empty backbone
    Tags
    FokI nuclease
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    July 24, 2012
    Availability
    Academic Institutions and Nonprofits only
    37184-plasmid-map-sequence-id-46727

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  6. pCAGGS-RaichuEV-Rac1(Y40C)

    Plasmid
    #164903
    Purpose
    Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation Y40C in the Rac1 domain. More useful than T17N mutation due to less harmful to endogenous signaling.
    Depositor
    Takeshi Imai
    Article
    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi
    Insert
    RaichuEV-Rac1 (RAC1 Human)
    Expression
    Mammalian
    Mutation
    Y40C mutation in the Rac1 domain whcih make the s…
    Available Since
    Aug. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    164903-plasmid-map-sequence-id-340567

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  8. pCAGGS-hPhyB-mCherry-HRasCT

    Plasmid
    #100281
    Purpose
    Expresses Phytochrome B (1-908 aa) fused with mCherry and Hras C-terminus in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Insert
    Phytochrome B (PHYB Mustard Weed)
    Tags
    mCherry fused with HRas C-terminus for plasma mem…
    Expression
    Mammalian
    Mutation
    Human codon optimized PhyB (1-908 aa)
    Promoter
    CAG promoter
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    100281-plasmid-map-sequence-id-197245

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  9. SpCas9-NG-APOBEC-nCas9-Ung

    Plasmid
    #198539
    Purpose
    Expresses human codon-optimized SpCas9-NG-APOBEC-nCas9-Ung and blasticidin resistance: EFS promoter-SpCas9-NG-APOBEC-nCas9-Ung-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Article
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-APOBEC-nCas9-Ung
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available Since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198539-plasmid-map-sequence-id-388890

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  10. pCambia-dLB-kanR-GFP

    Plasmid
    #223177
    Purpose
    Plant Transformation Vector that allows marker removal through Mendelian inheritance. Start of DNA transfer at RB, terminates at left border but in ~1/3 of cases goes ahead until second left border
    Depositor
    Andreas Stuermer
    Type
    Empty backbone
    Use
    Ti plasmid for agrobacterium
    Expression
    Plant
    Available Since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    223177-plasmid-map-sequence-id-443110

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  18. pCAGGS-SARS2-S-G614 (C-flag)

    Plasmid
    #156421
    Purpose
    Mammalian expression of SARS-CoV-2 S protein (G614) with the flag tag at C-terminus. Pseudotypes onto the MLV vector but not efficiently to VSV or lentiviral vectors.
    Depositor
    Hyeryun Choe, Michael Farzan
    Article
    Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:
    Insert
    SARS-CoV-2 S protein (S SARS CoV2)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    D614G
    Promoter
    CMV
    Available Since
    Aug. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    156421-plasmid-map-sequence-id-307829

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Showing: 1661 - 1680 of 5900 results