We narrowed to 5,560 results for: pcas

1. pCAGGS-PB1-AG(Y63L)-LOV2

   Plasmid

   #178876

   Purpose

   Mammalian expression of PB1-AzamiGreen(Y63L)-LOV2

   Depositor

   Shinya Tsukiji

   Article

   Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

   Insert

   PB1-AzamiGreen(Y63L)-LOV2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   AzamiGreen: Y63L

   Promoter

   CAG

   Available since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGGS-SARS2-S-FKO (C-flag)

   Plasmid

   #159364

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at C-terminus.

   Depositor

   Michael Farzan, Hyeryun Choe

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS-CoV-2)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Furin-cleavage site mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAGGS-RaichuEV-Rac1(T17N)

   Plasmid

   #164902

   Purpose

   Expresses a Rac1 FRET sensor RaichuEV-Rac1 which contains a dominant negative mutation T17N in the Rac1 domain

   Depositor

   Takeshi Imai

   Article

   Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

   Insert

   RaichuEV-Rac1 (RAC1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   T17N mutation in the Rac1 domain which make the s…

   Promoter

   Available since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-Flag-hsDicer (K70A)

   Plasmid

   #41589

   Depositor

   Phil Sharp

   Article

   Gurtan et al RNA. 2012 Jun;18(6):1116-22. Epub 2012 A

   Insert

   hsDicer (DICER1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   K70A

   Promoter

   CMV

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

5. 35S::Cel1SP-GeNL/pCAMBIA1301

   Plasmid

   #190069

   Purpose

   Expressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplast

   Depositor

   Takeharu Nagai

   Article

   Tran et al Plant Cell Environ. 2022 Jul 21. doi: 10

   Insert

   Cel1 signal peptide-Green-enhanced Nano-lantern
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   Feb. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9-Puro HLAG-2A-sgRNA

   Plasmid

   #182551

   Purpose

   Cas9 from S.pyogenes with 2A-Puro, and the 2A-sgRNA to targeting exon 2 of human HLA-G gene

   Depositor

   Santiago Miriuka

   Article

   Palma et al Sci Rep. 2021 Nov 12;11(1):22158. doi: 1

   Insert

   hSpCas9-2A-Puro-HLAG-2A-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   May 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-MBP-ATG9-P834A

   Plasmid

   #188082

   Purpose

   Expresses human ATG9-P834A mutant in mammalian cells

   Depositor

   James Hurley

   Article

   Ren et al Sci Adv. 2023 Feb 15;9(7):eadg2997. doi:

   Insert

   ATG9 (ATG9A Human)
   

   Use

   Tags

   MBP tag

   Expression

   Mammalian

   Mutation

   P834A

   Promoter

   CMV

   Available since

   Aug. 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair1

   Plasmid

   #98972

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNA targeting hLMNA. Generates breaks with 44bp 5' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 1 and Cas9(D10A)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Gma-pCAMBIA-1301-STTM171

   Plasmid

   #84080

   Purpose

   Disturb the expression of miR171 in soybean

   Depositor

   Guiliang Tang

   Article

   Yan et al Plant Cell. 2012 Feb;24(2):415-27. doi:

   Insert

   miR171
   

   Use

   Tags

   Expression

   Bacterial, Plant, and Yeast…

   Mutation

   Promoter

   2x35S

   Available since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-GFP-PQRv3-RFP

    Plasmid

    #73951

    Purpose

    Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.

    Depositor

    Brian Chen

    Article

    Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

    Inserts

    sfGFP (superfolder GFP)
    RFP
    

    Use

    Tags

    A residual PQR peptide wii be left at the C-termi…

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin and Chicken beta actin (shared…

    Available since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

11. pX330-Flag-dSpCas9-HF1

    Plasmid

    #92115

    Purpose

    Expression plasmid for human codon-optimized dead/inactive high-fidelity SpCas9-HF1 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive SpCas9-HF1 with FLAG tag
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    D10A, N497A, R661A, Q695A, H840A, Q926A

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-GBP1-10gly-lexADBD

    Plasmid

    #50795

    Purpose

    Expression of a GFP-dependent transcription factor component in mammalian cells

    Depositor

    Connie Cepko

    Article

    Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

    Insert

    GBP1-lexA DNA binding domain fusion protein
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-attBr-ZsGreen-attP

    Plasmid

    #51265

    Purpose

    Bxb1-responsive fluorescent reporter plasmid containing reverse complementary ZsGreen flanked by Bxb1 attB/P recognition sites in head-to-head orientation

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    attBr-ZsGreen-attP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-EGFP/RFP-int

    Plasmid

    #19818

    Depositor

    Zuoshang Xu

    Article

    Qiu et al BMC Biotechnol. 2008 . 8():77.

    Type

    Empty backbone

    Use

    Cre/Lox and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 21, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pCAG-Bgh-GFP-Farpl

    Plasmid

    #115501

    Purpose

    expression of rat Farp1

    Depositor

    Thomas Biederer

    Article

    Cheadle et al J Cell Biol. 2012 Dec 10;199(6):985-1001

    Insert

    Farp1 (Farp1 Rat)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    chicken beta-actin

    Available since

    Dec. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-LbCas12a-Plus-mCherry

    Plasmid

    #191230

    Purpose

    To express the LbCas12a nuclease variant "LbCas12a-Plus" that enhanced the editing activity and specificity

    Depositor

    Zhili Rong

    Article

    Huang et al BMC Biol. 2022 Apr 25;20(1):91. doi: 10.

    Insert

    LbCas12a-Plus-P2A-mCherry
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    D156R, R883K, R887A

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair2

    Plasmid

    #98973

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 96bp 5' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 2 and Cas9(D10A)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. PET-B-HypaSpCas9-NLS-6xHis

    Plasmid

    #207386

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    B-HypaSpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    N692A, M694A, Q695A, H698A, amino acids 1005-1013…

    Promoter

    T7

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET-deSpCas9-VP64-6xHis

    Plasmid

    #92117

    Purpose

    Expression of dead/inactive increased fidelity eSpCas9 (1.1)-VP64-6xHis in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    dead/inactive eSpCas9-NLS-3xFLAG-VP64
    

    Use

    CRISPR

    Tags

    3xFLAG, 6xHis, NLS, and VP64

    Expression

    Bacterial

    Mutation

    D10A, H840A, K848A, K1003A, R1060A

    Promoter

    T7

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCA24N-ligase-NLS-cTF

    Plasmid

    #87746

    Purpose

    IPTG-inducible expression of ligase-NLS-cTF fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Use

    Tags

    6xHis and NLS linker - cTF (chimeric transcriptio…

    Expression

    Bacterial

    Mutation

    Promoter

    T5-lac

    Available since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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