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Addgene

We narrowed to 17,223 results for: RET

Showing: 2551 - 2580 of 17223 results
  1. pcDNA3.1(+)GαsS-246-Nluc

    Plasmid
    #207157
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes short transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
    Insert
    GNAS (GNAS Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position D(246)N
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  2. pcDNA3.1(+)GαsL-260-NLuc

    Plasmid
    #207158
    Purpose
    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes long transcript variant of GNAS tagged with NanoLuciferase and is meant to be paired with Ggamma1 labeled with LSS-mKATE2
    Insert
    GNAS (GNAS Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLuc inserted at position D(260)N
    Promoter
    Available since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  3. FUW-crRNA-2-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP

    Plasmid
    #194894
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-2 targeting the human MECP2 locus.
    Depositor
    Inserts
    dLbCpf1-NLS-hCTCF_K365A_R368A
    U6-crRNA2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    Jan. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. FUW-crRNA1and2-U6_TRE-dLbCpf1-NLS-hCTCF_K365A_R368A-HA-P2A-tagBFP

    Plasmid
    #194886
    Purpose
    Dox-inducible all-in-one lentiviral construct to express dCpf1-CTCF-K365A_R368A mutant and crRNA-1 and -2 targeting the human MECP2 locus.
    Depositor
    Inserts
    dLbCpf1-NLS-hCTCF_K365A_R368A
    U6-crRNA1and2
    Use
    Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Human codon optimized catalytically inactive LbCp…
    Promoter
    TRE and U6
    Available since
    Jan. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. p35S_EVDm1

    Plasmid
    #167121
    Purpose
    Plant binary expression vector containing the coding sequence of the Arabidopsis Copia93 retroelement EVADE (AT5G17125) with U1 binding site mutated under CaMV 35S promoter.
    Insert
    EVADE (EVD) (AT5G17125 Mustard Weed)
    Use
    Tags
    Expression
    Plant
    Mutation
    mutated four nucleotides at the snoRNA U1 binding…
    Promoter
    CaMV 35S
    Available since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
  6. p35S_GFP-EVDinter-GUS

    Plasmid
    #167123
    Purpose
    Plant binary expression vector containing the intron and proximal plyadenylation signal sequences of the Copia93 retroelement EVADE (AT5G17125) between mGFP5 and GUS under CaMV 35S promoter.
    Insert
    EVADE GAG intron and terminator (EVD_in/ter) (AT5G17125 Mustard Weed)
    Use
    Tags
    GUS and mGFP5
    Expression
    Plant
    Mutation
    Promoter
    CaMV 35S
    Available since
    April 30, 2021
    Availability
    Academic Institutions and Nonprofits only
  7. pFUSEss-CHIg-mG3_VPEVS

    Plasmid
    #105863
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody has mutated 5 amino acids in lower hinge.
    Depositor
    Insert
    IgG3 heavy chain (Ighg3 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutated heavy chain of mouse IgG3 with five mutat…
    Promoter
    hEF1-HTLV prom
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  8. pFUSEss-CHIg-mG1_ILGGP

    Plasmid
    #105855
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody has mutated 5 amino acids in lower hinge.
    Depositor
    Insert
    IgG1 heavy chain (Ighg1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutated heavy chain of mouse IgG1 with five mutat…
    Promoter
    hEF1-HTLV prom
    Available since
    March 20, 2018
    Availability
    Academic Institutions and Nonprofits only
  9. pFUSEss-CHIg-mG1_CH2charge

    Plasmid
    #105852
    Purpose
    Expression plasmid coding for mutated heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutations modify charge of CH2 domain.
    Depositor
    Insert
    IgG1 heavy chain (Ighg1 Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    mutated heavy chain of mouse IgG1, muatations: Gl…
    Promoter
    hEF1-HTLV prom
    Available since
    March 19, 2018
    Availability
    Academic Institutions and Nonprofits only
  10. Myristoylated Kinase Library

    Plasmid Kit
    #1000000012
    Purpose
    190 human kinases and kinase-related open reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope tag to each ORF.
    Depositor
    Available since
    July 16, 2011
    Availability
    Academic Institutions and Nonprofits only
  11. pFUSE2ss-CLIg-mK_M18

    Plasmid
    #82358
    Purpose
    Expression plasmid coding for kappa light chain of mouse antibody specific to antigen B of the ABO blood group system, clone M18.
    Depositor
    Insert
    mouse immunoglobulin kappa light chain, variable fragment derived from clone M18 (Igk, Igkc, Igk-V Mouse)
    Use
    Tags
    none
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1-HTLV prom
    Available since
    Sept. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
  12. pMSCVpuro-Mito-Pericam

    Plasmid
    #87381
    Purpose
    Retroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.
    Depositor
    Insert
    Mito-Pericam [ratiometric]
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Retroviral 5' LTR promoter
    Available since
    June 7, 2018
    Availability
    Academic Institutions and Nonprofits only
  13. Epac-S-H188

    Plasmid
    #170349
    Purpose
    mT2Del_EPACdDEPCD_tdcp173Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.
    Depositor
    Insert
    mT2Del_EPACdDEPCD_tdcp173Ven(ST) (RAPGEF3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Deletion of DEP domain, Catalytically dead T781A …
    Promoter
    CMV
    Available since
    Oct. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
  14. GABA(A) receptor subunit a2SE

    Plasmid
    #49169
    Purpose
    pHluorin-tagged GABA A receptor subunit (alpha 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Insert
    GABA(A) receptor subunit alpha-2 (GABRA2 Human)
    Use
    Tags
    HA, bovine alpha 1 signal sequence, and pHGFP (pH…
    Expression
    Mammalian
    Mutation
    T343A
    Promoter
    CMV
    Available since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 2551 - 2580 of 17223 results