We narrowed to 6,134 results for: nls

1. pFUGW hUbC-Sin-dCas9-KRAB-T2A-GFP

   Plasmid

   #227010

   Purpose

   Lentiviral Expression of dCas9-KRAB from S. iniae fused to the repressive KRAB domain

   Depositor

   Charles Gersbach

   Article

   Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG, SV40 NLS

   Expression

   Mutation

   Promoter

   UbC

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pFUGW hUbC-Spa-dCas9-KRAB-T2A-GFP

   Plasmid

   #227011

   Purpose

   Lentiviral Expression of dCas9-KRAB from S. parasanguinis fused to the repressive KRAB domain

   Depositor

   Charles Gersbach

   Article

   Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG, SV40 NLS

   Expression

   Mutation

   Promoter

   UbC

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pFUGW hUbC-Sub-dCas9-KRAB-T2A-GFP

   Plasmid

   #227012

   Purpose

   Lentiviral Expression of dCas9-KRAB from S. uberis fused to the repressive KRAB domain

   Depositor

   Charles Gersbach

   Article

   Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG, SV40 NLS

   Expression

   Mutation

   Promoter

   UbC

   Available since

   Feb. 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MCP/PCP red RNA Lantern

   Plasmid

   #226097

   Purpose

   RNA tracking

   Depositor

   Jennifer Prescher

   Article

   Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

   Insert

   NLS-HA-MS2-SmBiT-IRES-PP7-mScarlet-I-LgBiT-FLAG
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TDP-43 mRuby K84ONBK

   Plasmid

   #216226

   Purpose

   TDP-43 with a C-terminal mRuby tag and Lysine 84 in the NLS mutated to a TAG stop codon for amber codon suppression.

   Depositor

   Jennifer Lee

   Article

   Shadish et al Biophys Chem. 2024 Jan 23;307:107191. do

   Insert

   Transactive response DNA binding protein of 43 kDa (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Lysine 84 mutated to a TAG stop codon

   Promoter

   CMV Promoter

   Available since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LLP782_Traptavidin-KRAB-CO

   Plasmid

   #211775

   Purpose

   AviTag counterpart binding domain, Traptavidin, fused to transcriptional repressor KRAB, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   SpyCatcher-DNMT3A
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect DNMT3A…

   Promoter

   pEF1a and pSV40

   Available since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. LLP785_Traptavidin-DNMT3A-CO

   Plasmid

   #211778

   Purpose

   AviTag counterpart binding domain, Traptavidin, fused to DNA methyltransferase DNMT3A, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Traptavidin-EZH2
   

   Use

   Tags

   3xV5

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect EZH2 e…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LLP781_SnoopCatcher-KRAB-CO

   Plasmid

   #211774

   Purpose

   SnoopTag counterpart binding domain, SnoopCatcher, fused to transcriptional repressor KRAB, with GFP selection

   Depositor

   Ryan Lister

   Article

   Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

   Insert

   Traptavidin-KRAB
   

   Use

   Tags

   3xV5

   Expression

   Mammalian

   Mutation

   Last 300 bp codon-optimised (CO) to detect KRAB e…

   Promoter

   pEF1a and pSV40

   Available since

   Dec. 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pROF018

   Plasmid

   #155325

   Purpose

   Constitutive expression of KRAB-EL222 in plants

   Depositor

   Matias Zurbriggen

   Article

   Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

   Insert

   PCaMV35S-NLS-KRAB-EL222-Tnos
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Oct. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pROF050

    Plasmid

    #155327

    Purpose

    Constitutive expression of BRD-EL222 in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-BRD-NLS-EL222-Tnos
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAB-HDAC5(mut-H-A)

    Plasmid

    #114397

    Purpose

    For PYL-HDAC5 H1006A expression

    Depositor

    Vincent Dion

    Article

    Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

    Insert

    PYL-HDAC5 H1006A
    

    Use

    Tags

    3xFlag-NLS (internal)

    Expression

    Mammalian

    Mutation

    D593E compared to NCBI ref NP_005465.2

    Promoter

    CMV

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAB-EXPR(PYL-cat_dom_HDAC5)

    Plasmid

    #114401

    Purpose

    For PYL-HDAC5 catalytic domain expression

    Depositor

    Vincent Dion

    Article

    Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

    Insert

    PYL-HDAC5 catalytic domain
    

    Use

    Tags

    3xFlag-NLS (internal)

    Expression

    Mammalian

    Mutation

    S220F and the deletion of M221

    Promoter

    CMV

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAB-EXPR(PYL-Nterm_dom_HDAC5)

    Plasmid

    #114402

    Purpose

    For PYL-HDAC5 N terminal domain expression

    Depositor

    Vincent Dion

    Article

    Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

    Insert

    PYL-HDAC5 N terminal domain
    

    Use

    Tags

    3xFlag-NLS (internal)

    Expression

    Mammalian

    Mutation

    D593E compared to NCBI ref NP_005465.2

    Promoter

    CMV

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAB(EXPR-PYL-HDAC5-NEO)

    Plasmid

    #114395

    Purpose

    For PYL-HDAC5 expression

    Depositor

    Vincent Dion

    Article

    Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

    Insert

    PYL-HDAC5
    

    Use

    Tags

    3xFlag-NLS (internal)

    Expression

    Mammalian

    Mutation

    D593E compared with NCBI reference NP_005465.2

    Promoter

    CMV

    Available since

    Aug. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pYPQ265K

    Plasmid

    #173997

    Purpose

    Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A) for C-G base editing

    Depositor

    Yiping Qi

    Article

    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d

    Insert

    UNG-rAPOBEC1(R33A)-zCas9(D10A)
    

    Use

    CRISPR

    Tags

    3X FLAG, NLS

    Expression

    Plant

    Mutation

    R33A in rAPOBEC1 and D10A in zCas9

    Promoter

    Available since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PlmdCas12e VPR

    Plasmid

    #188516

    Purpose

    Expresses FLAG tagged PlmdCas12e fused to VPR (VP64-p65-Rta)

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12e
    

    Use

    CRISPR and Lentiviral

    Tags

    NLS-FLAG-VPR

    Expression

    Mammalian

    Mutation

    D659A/E756A/D922A

    Promoter

    EF1a

    Available since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. KBB438

    Plasmid

    #185092

    Purpose

    GAL1::Rfa1[1-337, 344-621]-GFP with NLS removed

    Depositor

    Ken Belanger

    Article

    Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

    Insert

    RFA1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Rfa1 with amino acids 338-343 deleted

    Promoter

    GAL1

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAT15541_dCBE-SuperFi-Cas9

    Plasmid

    #184371

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dCBE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. L2_NOP1gRNA-Cas9-CsA

    Plasmid

    #136139

    Purpose

    Plasmid with NOP1-gRNA to tranform Mp as CRISPR control. Contains Cas9 and HygR

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    p5-35Sx2:HygR p5-MpU6:NOP1gRNA1 p5-MpEF1a:Cas9-NLS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Promoter

    Available since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJT174_GalL_A3A-Y130F-BE3

    Plasmid

    #145123

    Purpose

    Expressing base editor A3A-Y130F-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A(Y130F)-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(Y130F); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pJT173_GalL_A3A-R128A-BE3

    Plasmid

    #145122

    Purpose

    Expressing base editor A3A-R128A-BE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    A3A(R128A)-BE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    A3A(R128A); spCas9(D10A)

    Promoter

    GalL

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJT113_GalL_cCDA1-xBE3

    Plasmid

    #145079

    Purpose

    Expressing base editor cCDA1-xBE3 in yeast cells

    Depositor

    Ralph Bock

    Article

    Tan et al Nat Commun. 2020 Jan 31;11(1):629. doi:

    Insert

    cCDA1-xBE3
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9(D10A, A262T, R324L, S409I, E480K, E543D, M…

    Promoter

    GalL

    Available since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pVAX_CBI_AcrIIA6_DT1

    Plasmid

    #136668

    Purpose

    Expresses human codon-optimized AcrIIA6 DT1 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

    Depositor

    Yannick Doyon

    Article

    Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

    Insert

    AcrIIA6_DT1
    

    Use

    Synthetic Biology

    Tags

    No tag, No NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pVAX_CBI_AcrIIA6_D1811

    Plasmid

    #136667

    Purpose

    Expresses human codon-optimized AcrIIA6 D1811 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

    Depositor

    Yannick Doyon

    Article

    Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

    Insert

    AcrIIA6_D1811
    

    Use

    Synthetic Biology

    Tags

    No tag, No NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pVAX_CBI_AcrIIA6_D1024

    Plasmid

    #136666

    Purpose

    Expresses human codon-optimized AcrIIA6 D1024 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

    Depositor

    Yannick Doyon

    Article

    Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

    Insert

    AcrIIA6_D1024
    

    Use

    Synthetic Biology

    Tags

    No tag, No NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pVAX_CBI_AcrIIA5_D4276

    Plasmid

    #136664

    Purpose

    Expresses human codon-optimized AcrIIA5 D4276 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

    Depositor

    Yannick Doyon

    Article

    Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

    Insert

    AcrIIA5_D4276
    

    Use

    Synthetic Biology

    Tags

    No tag, No NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pVAX_CBI_AcrIIA5_D1126

    Plasmid

    #136663

    Purpose

    Expresses human codon-optimized AcrIIA5 D1126 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

    Depositor

    Yannick Doyon

    Article

    Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

    Insert

    AcrIIA5_D1126
    

    Use

    Synthetic Biology

    Tags

    No tag, No NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ARB340

    Plasmid

    #124023

    Purpose

    Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear iRFP713

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    LS-A4_EF1a-NLS::iRFP713-bgPA_R1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. ARB341

    Plasmid

    #124024

    Purpose

    Encodes TU with connectors LS R1 and an A4 insulator, and pEF1a expressing nuclear tagBFP

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    LS-A4_EF1a-NLS::tagBFP-bgPA_R1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. JPF0507

    Plasmid

    #123998

    Purpose

    Encodes pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pPGK1-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. JPF0508

    Plasmid

    #123999

    Purpose

    Encodes pCAG driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCAG-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. JPF0509

    Plasmid

    #124000

    Purpose

    Encodes pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pSV40-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. JPF0513

    Plasmid

    #124004

    Purpose

    Encodes pCMV-A driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCMV-A-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. JPF0514

    Plasmid

    #124005

    Purpose

    Encodes pCMV-B driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCMV-B-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. JPF0515

    Plasmid

    #124006

    Purpose

    Encodes pCMV-C driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCMV-C-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. JPF0516

    Plasmid

    #124007

    Purpose

    Encodes pCMV-D driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCMV-D-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. JPF0517

    Plasmid

    #124008

    Purpose

    Encodes pCMV-E driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCMV-E-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. JPF0518

    Plasmid

    #124009

    Purpose

    Encodes pCMV-F driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCMV-F-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. JPF0519

    Plasmid

    #124010

    Purpose

    Encodes pCMV-G driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pCMV-G-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. JPF0521

    Plasmid

    #124012

    Purpose

    Encodes crippled pPGK1 driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pPGK1c-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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41. JPF0523

    Plasmid

    #124014

    Purpose

    Encodes crippled pSFFV driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    PB_pSFFVc-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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42. MTK4a_009

    Plasmid

    #123956

    Purpose

    Encodes a nuclear localized EBFP2 (T2A) coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    T2A::NLS::EBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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43. MTK4a_010

    Plasmid

    #123823

    Purpose

    Encodes a nuclear localized mRuby2 coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    T2A::NLS::mRuby2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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44. MTK4a_011

    Plasmid

    #123824

    Purpose

    Encodes a nuclear localized iRFP670 coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    T2A::NLS::iRFP670
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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45. MTK4a_013

    Plasmid

    #123826

    Purpose

    Encodes a nuclear localized iRFP713 coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    P2A::NLS::iRFP713
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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46. MTK4a_016

    Plasmid

    #123829

    Purpose

    Encodes a nuclear localized tagBFP coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    P2a-NLS::tagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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47. MTK4a_017

    Plasmid

    #123830

    Purpose

    Encodes a nuclear localized EBFP2 coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    P2a-NLS::EBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. MTK4a_018

    Plasmid

    #123831

    Purpose

    Encodes a nuclear localized mTurquoise2 coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    P2a-NLS::mTurquoise
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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49. MTK4a_021

    Plasmid

    #123834

    Purpose

    Encodes a nuclear localized and destabilized mRuby2 coexpression cassette as a Type 4a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    T2A::NLS::mRuby2::PEST4d
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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50. MTK3a_024

    Plasmid

    #123776

    Purpose

    Encodes spCAS9 tagged with NLS as a Type 3a part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    spCAS9-2xNLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge