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pAAVS1-Nst-MCS

Plasmid

#80487

Purpose

donor vector for AAVS1 targeting (neomycin selection) with multiple cloning site

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-SMVP-Cas9N-U6-gRNA P1

Plasmid

#80943

Purpose

Expresses Cas9N in mammalian cells; expresses gRNA P1 for Pd-l1 binding.

Depositor

George Church

Publication

Chew et al Nat Methods. 2016 Sep 5. doi: 10.1038/nm

Insert

Cas9N

Use

AAV, CRISPR, and Synthetic Biology

Expression

Mammalian

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-HTB

Plasmid

#44187

Depositor

Edward Callaway

Insert

Histone tagged GFP, T2A-TVA-E2A-B19G

Use

AAV; Adeno-associated vector

Tags

GFP

Promoter

EF1a

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pAAVS1-P-CAG-mCh

Plasmid

#80492

Purpose

donor vector for AAVS1 targeting (puromycin selection) and constitutive mCherry expression

Depositor

Knut Woltjen

Publication

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Insert

mCherry

Use

Donor vector (human)

Expression

Mammalian

Promoter

CAG

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pZT-AAVS1-R1

Plasmid

#52638

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Publication

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Right AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available since

May 12, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pAAV.hSyn1.Twitch2B.WPRE.SV40

Plasmid

#100040

Purpose

AAV expression of Twitch2B driven from hSyn1 promoter. Twitch 2B is a fluorescent reporter for calcium signaling

Depositor

Oliver Griesbeck

Publication

Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

Has service

AAV1

Insert

Twitch2B

Use

AAV

Expression

Mammalian

Promoter

hSyn1

Available since

Jan. 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMGS7 (AAVS1sgRNA)

Plasmid

#126582

Purpose

A CRISPR/Cas9 construct to target the AAVS1 locus in humans

Depositor

Michael Guertin

Publication

Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

Insert

AAVS1 sgRNA

Expression

Mammalian

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pAAVS1-PC-GCaMP6f

Plasmid

#73503

Purpose

GCaMP6f knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Publication

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

GCaMP6f

Expression

Mammalian

Promoter

CAG

Available since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pGolden-AAV

Plasmid

#51424

Purpose

Contain AAV ITRs, lacZ expressing for blue-white selection for golden-gate reaction

Depositor

Yonglun Luo

Publication

Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

Insert

lacZ

Use

AAV

Promoter

lacZ

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pZT-AAVS1-L1

Plasmid

#52637

Purpose

TALEN-mediated gene editing at human PPP1R12C/AAVS1 locus

Depositor

Mahendra Rao, Jizhong Zou

Publication

Luo et al Stem Cells Transl Med. 2014 May 15. pii:

Insert

Left AAVS1 TALEN

Use

TALEN

Expression

Mammalian

Promoter

CMV

Available since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pACAGW-oChiEFtdTomato-AAV

Plasmid

#32846

Purpose

AAV expression of mammalian codon optimized ChIEF for optogenetic excitation.

Depositor

Rory McQuiston

Publication

Jurgens et al PLoS One. 2012;7(9):e45717. doi: 10.1371

Insert

oChiEF

Use

AAV

Tags

tdTomato

Promoter

CAGG

Available since

March 19, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pENN.AAV.CAG.Flex.CaMPARI.WPRE.SV40

Plasmid

#100831

Purpose

AAV expression of CaMPARI, a photoconvertible fluorescent protein-based calcium integrator

Depositor

Loren Looger, Eric Schreiter

Publication

Fosque et al Science. 2015 Feb 13;347(6223):755-60. d

Insert

CaMPARI

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVS1P-iCAG.copGFP

Plasmid

#66577

Purpose

AAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-AAVS1 TALENs

Depositor

Jizhong Zou

Publication

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

copGFP

Expression

Mammalian

Promoter

CAG

Available since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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PGK-AAVS1ZFNR

Plasmid

#60915

Purpose

encodes zinc finger nuclease specific to AAVS1 locus (right)

Depositor

Paul Gadue

Publication

Sim et al Methods Mol Biol. 2015 Jan 29.

Insert

AAVS1-ZFNR

Promoter

PGK

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV asyn S129A

Plasmid

#36066

Depositor

Patrick Aebischer, Hilal Lashuel

Insert

alpha-synuclein S129A (SNCA Human)

Use

AAV

Expression

Mammalian

Mutation

S129A

Promoter

CMV

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pZE21-GFPaav

Plasmid

#26643

Depositor

Michael Elowitz

Publication

Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

Insert

pZE21-GFPaav

Tags

GFPaav

Expression

Bacterial

Mutation

na

Available since

June 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV-flex-oChIEF-citrine

Plasmid

#50973

Purpose

flexed oChIEF-citrine construct in an AAV transfer vector containing AAV2 ITR

Depositor

Roger Tsien

Publication

Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

Insert

ChIEF-citrine

Use

AAV

Tags

citrine

Mutation

inverted and inserted between two lox sites

Promoter

human synapsin promoter

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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PGK-AAVS1ZFNL

Plasmid

#60916

Purpose

encodes zinc finger nuclease specific to AAVS1 locus (left)

Depositor

Paul Gadue

Publication

Sim et al Methods Mol Biol. 2015 Jan 29.

Insert

AAVS1-ZFNL

Promoter

PGK

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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hAAVS1 TALEN Right

Plasmid

#52342

Purpose

TALEN vector for genomic targeting

Depositor

Su-Chun Zhang

Publication

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

AAVS1 TALEN Right

Expression

Mammalian

Promoter

CMV

Available since

July 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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hAAVS1 TALEN Left

Plasmid

#52341

Purpose

TALEN vector for genomic targeting

Depositor

Su-Chun Zhang

Publication

Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

Insert

AAVS1 TALEN Left

Expression

Mammalian

Promoter

CMV

Available since

July 19, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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