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Addgene

We narrowed to 1,833 results for: pb

Showing: 1721 - 1740 of 1833 results
  1. PB09-TRE-beta-globin-miR-ctrl-EF1a-GFP

    Plasmid
    #117319
    Purpose
    Overexpression of miR-124-surrounding locus (not miR-124) in eurkaryotic cells, encoded in a human beta-globin intron
    Depositor
    Insert
    hsa-miR-CTRL
    Use
    Transposon-mediated integration
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    miR-124 deleted
    Promoter
    Tet-inducible promoter
    Available since
    Jan. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pHD-sfGFP-ScarlessDsRed-GMR-eya(shRNA)

    Plasmid
    #240228
    Purpose
    Parental donor plasmid for scarless sfGFP-fusion knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  3. pHD-T2A-Gal4-3xP3DsRed-GMR-eya(shRNA)

    Plasmid
    #240229
    Purpose
    Parental donor plasmid for T2A-Gal4 knock-in fly lines. Red fluorescent fly eye marker to select for knock-in events, small fly eye marker to select against backbone integration.
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    July 11, 2025
    Availability
    Academic Institutions and Nonprofits only
  4. pegRNA entry vector - pUC19-U6-[BsmBI_entry]-term (MNW320)

    Plasmid
    #208977
    Purpose
    Entry vector for human U6 promoter driven SpCas9-based pegRNAs, comprised of hU6-[BsmBI]-terminator (spacer and RTT/PBS oligos must be cloned in)
    Insert
    pUC19-U6-[BsmBI]-term (pegRNA_entry_vector)
    Use
    CRISPR
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Promoter
    human U6
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
  5. pPB-tetO(hCMV1)-HA-Tet1(201R2)-IV

    Plasmid
    #102421
    Purpose
    Inducible expression of siRNA resistant mouse Tet1-201 (Ensembl transcript ENSMUST00000050826.13) with HA-tag and IRES-Venus in piggyBac (PB) transposon vector
    Depositor
    Insert
    Tet1 (Tet1 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Modified at the Dharmacon SMARTpool siRNA #2 targ…
    Promoter
    TetO-CMV
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
  6. pPB-tetO(hCMV1)-HA-Tet1mHxD(201R2)-IV

    Plasmid
    #102422
    Purpose
    Inducible expression of siRNA resistant mouse Tet1-201 (ENSMUST00000050826.13) with mutated catalytic domain (H1620Y & D1622A), HA-tag and IRES-Venus in piggyBac (PB) transposon vector
    Depositor
    Insert
    Tet1 catalytic domain mutant (Tet1 Mouse)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    H1620Y and D1622A mutations in Tet1 catalytic dom…
    Promoter
    TetO-CMV
    Available since
    Nov. 20, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 1721 - 1740 of 1833 results