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SlugABEmax

Plasmid

#163798

Purpose

Expresses ABEmax and nSlugCas9

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Mutation

SlugCas9 (D10A)

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-flx-fDIO-HA-Rpl10a-T2A-myc-hM3D(Gq)

Plasmid

#223654

Purpose

Expresses vTRAP and hM3D(Gq) in a FlpON-CreOFF dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

vTRAP-T2A-hM3D(Gq)

Use

AAV

Tags

HA

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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SlugBE4max

Plasmid

#163799

Purpose

Expresses AncBE4max and nSlugCas9

Depositor

Yongming Wang

Article

Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Mutation

SlugCas9 (D10A)

Available Since

April 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTagRFP657-C1

Plasmid

#31872

Purpose

For fusion of TagRFP657 to N-term of insert. SEE DEPOSITOR COMMENTS BELOW.

Depositor

Vladislav Verkhusha

Article

Morozova et al Biophys J. 2010 Jul 21;99(2):L13-5.

Insert

TagRFP657

Expression

Mammalian

Mutation

M44Q, K69H, F84W, S148H, S165T, D166A, M167L, L18…

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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mscv2.2-NLRC4

Plasmid

#60199

Purpose

Mouse NLRC4 expressed under the constitutive, moderate-level retroviral LTR promoter, expresses in mammalian cells; IRES-GFP reporter downstream of NLRC4

Depositor

Russell Vance

Article

Kofoed et al Nature. 2011 Aug 28;477(7366):592-5. doi

Insert

NLRC4 (Nlrc4 Mouse)

Use

Retroviral

Expression

Mammalian

Promoter

LTR

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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BPK3082

Plasmid

#78742

Purpose

Human expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

LbCpf1 crRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pLSSmOrange-mKate2

Plasmid

#99868

Purpose

Measure FRET between LSSmOrange and mKate2

Depositor

Marc Tramier

Article

Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10

Insert

LSSmOrange

Tags

mKate2

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2017

Availability

Academic Institutions and Nonprofits only

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LDB-3-p65

Plasmid

#158984

Purpose

Expresses LDB-3 fused with a C-terminal p65 transcriptional activation domain in mammalian cells

Depositor

Liangcai Gu

Article

Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

Insert

LDB-3-p65

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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miReporter-PGK

Plasmid

#82477

Purpose

microRNA reporter relying on a bidirectional PGK promoter. Contains MCS downstream of H2B-mCherry and H2B-Citrine to insert miRNA binding sites. Contains a Hygromycin selection cassette.

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2016 May 6;11(5):e0155177. doi

Insert

H2B-mCherry and H2B-Citrine. HygroR

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pMAL-his-LbCpf1-EC

Plasmid

#79008

Purpose

Bacterial expression - MBP-his tagged LbCpf1 (E.coli codon optimized)

Depositor

Jin-Soo Kim

Article

Kim et al Nat Biotechnol. 2016 Jun 6. doi: 10.1038

Insert

LbCpf1

Tags

MBP-6xHis-NLS

Expression

Bacterial

Promoter

tac promoter

Available Since

July 1, 2016

Availability

Academic Institutions and Nonprofits only

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qz5

Plasmid

#25867

Depositor

Bruce Conklin

Article

Conklin et al Nature. 1993 May 20. 363(6426):274-6.

Insert

Gq alpha (Gnaq Mouse)

Tags

HA (see comments)

Expression

Mammalian

Mutation

Replacement of the five carboxyl-terminal amino a…

Available Since

Aug. 25, 2010

Availability

Academic Institutions and Nonprofits only

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pMA2640

Plasmid

#25434

Purpose

Retroviral Tet-On vector for CMV-driven rtTA with EGFP and Blasticidin selection

Depositor

Mikhail Alexeyev

Article

Alexeyev et al Mol Biol Rep. 2010 Apr . 37(4):1987-91.

Insert

reverse tetracycline-regulated transactivator Advanced

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pBI-eGFP-Rab31

Plasmid

#200265

Purpose

Expression of eGFP-Rab31 (human) in mammalian cells

Depositor

Guangpu Li

Article

Banworth et al J Biol Chem. 2022 Sep;298(9):102281. doi

Insert

Rab31 (human cDNA) (RAB31 Human)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 25, 2023

Availability

Academic Institutions and Nonprofits only

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mEGFP-RhoA-C1

Plasmid

#29674

Depositor

Ryohei Yasuda

Article

Murakoshi et al Nature. 2011 Apr 7;472(7341):100-4. doi:

Insert

RhoA (RHOA Human)

Tags

mEGFP

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only

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pTRIPZ TREX1-KI

Plasmid

#127701

Purpose

For knocking in TREX1 in mouse - Doxycyclin inducible

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

mouse TREX1 cDNA (Trex1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-RhoA wt

Plasmid

#11750

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

RhoA (RHOA Human)

Tags

Flag

Expression

Mammalian

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
LDB-14-p65

Plasmid

#158985

Purpose

Expresses LDB-14 fused with a C-terminal p65 transcriptional activation domain in mammalian cells

Depositor

Liangcai Gu

Article

Huang et al ACS Synth Biol. 2020 Nov 12. doi: 10.102

Insert

LDB-14-p65

Tags

V5 tag , 6xHis tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp3-His

Plasmid

#11821

Depositor

Guy Salvesen

Article

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 3 (CASP3 Human)

Tags

6xHis

Expression

Bacterial

Available Since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

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pUC19_PHF1_HTC3xF_BSD_donor

Plasmid

#246394

Purpose

PHF1 donor plasmid with blasticidin resistance

Depositor

Yicheng Long

Article

Much et al Mol Cell. 2025 Aug 7:S1097-2765(25)00615

Insert

PHF1 (PHF1 Human)

Tags

HaloTag_3XFLAG

Expression

Mammalian

Available Since

Nov. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pTK305

Plasmid

#66812

Purpose

IVT vector for Fluc mRNA

Depositor

Ron Weiss

Article

Andries et al J Control Release. 2015 Sep 2. pii: S016

Insert

Firefly luciferase

Use

Luciferase; Mrna ivt

Promoter

T7

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

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