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pAAV-CAG-soCoChR-GFP

Plasmid

#107710

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CAG promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Promoter

CAG

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-soCoChR-mCardinal

Plasmid

#107713

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. hSynapsin promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Insert

soCoChR-mCardinal

Use

AAV

Tags

KA2 and mCardinal

Expression

Mammalian

Promoter

hSyn

Available Since

May 1, 2018

Availability

Academic Institutions and Nonprofits only

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HOXA7 (murine) His-tag pET

Plasmid

#8491

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA7 (Hoxa7 Mouse)

Tags

His and T7

Expression

Bacterial

Mutation

Fusion sequence is: ggatccATG; makes protein by …

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
p53-(1-393)-R273H-N268D

Plasmid

#35087

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains aa 1-393 - R273H-N268D

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
p164 Dync1i1.B

Plasmid

#26435

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 1 isoform B (Dync1i1 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
HOXA4 (murine) HA-tag pET

Plasmid

#8489

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXA4 (Hoxa4 Mouse)

Tags

HA and T7

Expression

Bacterial

Mutation

Thr-2 changed to Ala-2 to form better ribosomal b…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pNH12 (pmyo-2::MacQ::mCitrine)

Plasmid

#130274

Purpose

Expresses the eFRET-based voltage sensor MacQ::mCitrine in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Article

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

MacQ::mCitrine

Tags

mCitrine

Expression

Worm

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-Atoh1wt-IRES-RFP

Plasmid

#33334

Depositor

Martine Roussel, Charles Sherr

Article

Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
HOXA11 (murine) HIS-tag pET

Plasmid

#8517

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol 1997 Nov;17(11):6448-58.

Insert

HOXA11 (Hoxa11 Mouse)

Tags

T7

Expression

Bacterial

Mutation

makes protein by Western

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HOXB2 (human) HIS-tag pET

Plasmid

#8522

Depositor

Corey Largman

Article

Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

Insert

HOXB2 (HOXB2 Human)

Tags

His and T7

Expression

Bacterial

Mutation

Starts at AA #2; makes protein by Western

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pGEX-6P-1-hs-ALPS 1-ALPS2-mCherry

Plasmid

#187114

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSG00000101199 (ARFGAP1 Human)

Tags

mCherry

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pET30a-SARS-CoV2-ORF8

Plasmid

#165089

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)

Expression

Bacterial

Available Since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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p199 Dync1i2.C (Ex1a)

Plasmid

#26449

Depositor

Elizabeth Fisher

Article

Kuta et al PLoS One. 2010 . 5(7):e11682.

Insert

cytoplasmic dynein 1 intermediate chain 2 isoform C (exon 1a) (Dync1i2 Mouse)

Use

Subcloning and sequencing

Mutation

N/A

Available Since

March 24, 2011

Availability

Academic Institutions and Nonprofits only
p164-pAAV-mDlx-FLEX-mScarlet

Plasmid

#204689

Purpose

Fluorescent reporter under the control of the Dlx promotor

Depositor

Lynette Lim

Article

Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

Insert

mScarlet

Use

AAV and Cre/Lox

Tags

mScarlet palmitoylation

Expression

Mammalian

Promoter

mDlx

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCV-Atoh1wt-IRES-GFP

Plasmid

#33333

Depositor

Martine Roussel, Charles Sherr

Article

Ayrault et al Cancer Res. 2010 Jul 1;70(13):5618-27. E

Insert

Atoh1 (Atoh1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
XW17

Plasmid

#65835

Purpose

Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

OR74A quinic acid utilization activator (qa-1F)

Tags

mCherry

Expression

Worm

Mutation

The endogenous SphI, KpnI and SacI sites in QF ar…

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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XE123 XDsh delta PDZ-GFP- CS2+

Plasmid

#16786

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

Insert

Xdsh-delta-PDZ (dvl2.L Frog)

Use

Xenopus expression

Tags

gfp

Mutation

contains Dsh amino acids 1-267 and 381-736 (delet…

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
p53-(1-393)-E285K-N268D

Plasmid

#35089

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

p53 (TP53 Human)

Tags

His and Thrombin cleavage site

Expression

Bacterial

Mutation

Contains aa 1-393 with N269D and E285K mutations

Promoter

T7

Available Since

April 4, 2012

Availability

Academic Institutions and Nonprofits only

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pLNCX2-Orai1

Plasmid

#89818

Purpose

Express Orai1 in mammalian cells

Depositor

Shengyu Yang

Article

Sun et al J Cell Biol. 2014 Nov 24;207(4):535-48.

Insert

Orai1 (ORAI1 Human)

Use

Retroviral

Available Since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pFastBac HT JS-Munc18b

Plasmid

#135554

Purpose

Express rat Munc18b in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18b.

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

Munc18b (Stxbp2 Rat)

Tags

6xHis tag and TEV site

Expression

Insect

Promoter

polyhedrin promoter

Available Since

March 17, 2020

Availability

Academic Institutions and Nonprofits only

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