Addgene

We narrowed to 13,155 results for: Mpl;

Showing: 3521 - 3560 of 13155 results

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pS3C

Plasmid

#108211

Purpose

Expresses SsoPCNA3-CYP101A1 fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

SsoPCNA3-CYP101A1 fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pM3C

Plasmid

#108212

Purpose

Expresses MsePCNA3-CYP101A1 fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

MsePCNA3-CYP101A1 fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pM1R

Plasmid

#108208

Purpose

Expresses MsePCNA1-PdR fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

MsePCNA1-PdR fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pS1R

Plasmid

#108207

Purpose

Expresses SsoPCNA1-PdR fusion protein in E. coli

Depositor

Teruyuki Nagamune

Article

Iwata et al Biotechnol J. 2018 Nov;13(11):e1700662.

Insert

SsoPCNA1-PdR fusion protein

Use

Tags

His6 tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAS2-1-Brr2

Plasmid

#99995

Purpose

Express yeast Brr2 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Brr2 (BRR2 Budding Yeast)

Use

Tags

Y2H binding domain

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pACT2-Rcl1

Plasmid

#99992

Purpose

Express yeast Rcl1 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Rcl1 (RCL1 Budding Yeast)

Use

Tags

Y2H activation domain

Expression

Yeast

Mutation

Contains amino acids 1-217 of Rcl1

Promoter

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pACT2-Kre33

Plasmid

#99990

Purpose

Express yeast Kre33 with Y2H activation domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Kre33 (KRE33 Budding Yeast)

Use

Tags

Y2H activation domain

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pAS2-1-Kre33

Plasmid

#99989

Purpose

Express yeast Kre33 with Y2H binding domain fusion

Depositor

Susan Baserga

Article

Vincent et al RNA. 2017 Oct 20. pii: rna.062927.117. d

Insert

Kre33 (KRE33 Budding Yeast)

Use

Tags

Y2H binding domain

Expression

Yeast

Mutation

Promoter

Available since

Nov. 20, 2017

Availability

Academic Institutions and Nonprofits only
pCML 220

Plasmid

#98588

Purpose

pCYFP. Expresses CYFP control fusion in E. coli for TriFC assay (kanR).

Depositor

Lydia Contreras

Article

Gelderman et al Biotechnol Bioeng. 2015 Feb;112(2):365-7

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLacO

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pAP1071-2

Plasmid

#99502

Purpose

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3utr::linker::TEV

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::meGFP with 2 introns::Ollas::H2B::tbb-2 3’utr::linker::TEV

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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LPhP-pHG165

Plasmid

#90047

Purpose

Constitutive expression of a chimeric LacI:PurR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Tungtur et al J Mol Biol. 2010 Jan 29;395(4):785-802.

Insert

LPhP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Iq

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a_Msm_RbpA

Plasmid

#87928

Purpose

Mycobacterium smegmatis RpbA in pET21a (ampR) used to co-express with Msm SigA for crystallization purposes

Depositor

Seth Darst

Article

Hubin et al Elife. 2017 Jan 9;6. pii: e22520. doi: 1

Insert

RbpA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7P

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-GST-Laforin C329S

Plasmid

#84682

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin C278S

Plasmid

#84681

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin C266S

Plasmid

#84680

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin C250S

Plasmid

#84679

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin C205S

Plasmid

#84678

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin C169S

Plasmid

#84677

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin 2CS (C266S,C278S)

Plasmid

#84674

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc 241X

Plasmid

#84658

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Contains residues 1-240 only

Promoter

5'LTR

Available since

Feb. 6, 2017

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin 3CS (C250S,C266S,C278S)

Plasmid

#84676

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only
pGEX6P1-GST-Laforin 2CS_new (C278S, C329S)

Plasmid

#84675

Purpose

bacterial expression, GST-fusion protein

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc K87A

Plasmid

#84670

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc K320A

Plasmid

#84669

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc 2CS_new (C278S, C329S)

Plasmid

#84659

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Dec. 2, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc C266S

Plasmid

#84666

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc (C266S,C278S)

Plasmid

#84657

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc C329S

Plasmid

#84668

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc C278S

Plasmid

#84667

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc C169S

Plasmid

#84663

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc C205S

Plasmid

#84664

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc C250S

Plasmid

#84665

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc W32G

Plasmid

#84672

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only
pQCXIP-Laforin Myc 2KA (K87A, K320A)

Plasmid

#84660

Purpose

mammalian expression and/or retrovirus preparation

Depositor

Ayano Satoh

Article

Toyota et al Sciencematters

Insert

Laforin (EPM2A Human)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Promoter

5'LTR

Available since

Nov. 24, 2016

Availability

Academic Institutions and Nonprofits only
pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFP

Plasmid

#84253

Purpose

Expresses Sp sgCD95-3 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-3

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFP

Plasmid

#84252

Purpose

Expresses Sp sgCD95-2 gRNA with an EGFP fluorescent marker

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Insert

Sp sgCD95-2

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA4/TO-myc-mCE

Plasmid

#82470

Purpose

Expression of myc epitope and murine mRNA Capping Enzyme in mammalian cells

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

Capping Enzyme (Rngtt Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 15, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE63

Plasmid

#79445

Purpose

recipient plasmid [single nuclease]; p35S:Cas9, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE144

Plasmid

#79448

Purpose

recipient plasmid [multiplexing]; p35S:Cas9, FAST, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE148

Plasmid

#79450

Purpose

recipient plasmid [multiplexing]; pDD45:Cas9, FAST, BASTA

Depositor

Johannes Stuttmann

Article

Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

Insert

ccdB (ccdB(letD) )

Use

CRISPR

Tags

Expression

Plant

Mutation

BsaI site eliminated

Promoter

Available since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 3521 - 3560 of 13155 results

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