We narrowed to 5,570 results for: donor

1. AICSDP-26:ST6GAL1-mEGFP

   Plasmid

   #101786

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human ST6GAL1

   Depositor

   Allen Institute for Cell Science

   Insert

   ST6GAL1 Homology Arms with linker-mEGFP (ST6GAL1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Oct. 31, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pJet1.2 cat cassette

   Plasmid

   #117119

   Purpose

   Chloramphenicol resistance cassette

   Depositor

   Stephan Gruber

   Article

   Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

   Insert

   Chloramphenicol resistance cassette for Bacillus subtilis selection
   

   Use

   Golden gate donor vector for gene targeting in ba…

   Available Since

   Nov. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRVV661

   Plasmid

   #108282

   Purpose

   Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

   Depositor

   Richard Mann

   Article

   Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

   Insert

   PhiC31 attP sites
   

   Use

   CRISPR

   Available Since

   May 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRVV669

   Plasmid

   #108283

   Purpose

   Donor vector for creation of PhiC31 attP-flanked platforms for cassette exchange using CRISPR/Cas9

   Depositor

   Richard Mann

   Article

   Voutev et al G3 (Bethesda). 2018 Mar 9. pii: g3.118.2

   Insert

   PhiC31 attP sites
   

   Use

   CRISPR

   Available Since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. LT1_34_sfpHluorin

   Plasmid

   #177707

   Purpose

   Plasmid for genome integration in X2 site expressing sfpHluorin (intracellular pH biosensor)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Insert

   pTEF1-sfpHluorin
   

   Use

   Used as donor in genome integration after lineari…

   Expression

   Yeast

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMLS253

   Plasmid

   #114271

   Purpose

   SapTrap donor vector for point mutagenesis; pENTR-221::syntron(loxp:unr-119+)

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al MicroPubl Biol. 2018 Jul 29;2018:10.1791

   Insert

   Cbr-Unc-119
   

   Use

   CRISPR and Cre/Lox

   Mutation

   *please see depositor comment below

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. AICSDP-57: SMC1A-mEGFP

   Plasmid

   #114406

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human SMC1A

   Depositor

   Allen Institute for Cell Science

   Insert

   SMC1A Homology Arms with linker-mEGFP (SMC1A Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Sept. 4, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. AICSDP-117:POLR2A-mEGFP

   Plasmid

   #164500

   Purpose

   Homology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2A

   Depositor

   Allen Institute for Cell Science

   Insert

   POLR2A Homology Arms with mEGFP-linker (POLR2A Human)
   

   Use

   CRISPR; Donor template

   Tags

   mEGFP-linker

   Expression

   Mammalian

   Mutation

   homology arms contain point mutations to disrupt …

   Available Since

   Feb. 17, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. LT1_33_pTEFmut8-mCherry

   Plasmid

   #177706

   Purpose

   Construct for genome integration expressing constitutively mCherry under pTEFmut8 promoter

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Insert

   pTEFmut8-mCherry
   

   Use

   Used as donor dna in genome integration after lin…

   Expression

   Yeast

   Available Since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJET1.2-STOP-dsRed

    Plasmid

    #60944

    Purpose

    Drosophila, Splicing acceptor, STOP, dsRED, RFP, SV40 terminator, flanked attP. section marker, assemble with homology arms to make Donor plasmid for CRISPR.

    Depositor

    Frank Schnorrer

    Article

    Zhang et al G3 (Bethesda). 2014 Oct 15. pii: g3.114.

    Insert

    dsRED
    

    Use

    Unspecified

    Promoter

    3XP3

    Available Since

    Nov. 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. AICSDP-69: NPM1-mTagRFP-T

    Plasmid

    #124608

    Purpose

    Homology arms and linker-mTagRFP-T sequence for C-terminus tagging of human NPM1

    Depositor

    Allen Institute for Cell Science

    Insert

    NPM1 Homology Arms with linker-mTagRFP-T (NPM1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    May 13, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. p5E_(-3kb)nrg1 type II

    Plasmid

    #199393

    Purpose

    pDEL61; Gateway 5' Entry clone encoding nrg1 type II upstream regulatory sequence

    Depositor

    William Talbot

    Article

    Lysko et al Cell Rep. 2022 Nov 15;41(7):111669. doi:

    Insert

    (-3kb)nrg1 type II
    

    Use

    Gateway donor vector

    Available Since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJet1.2 specR cassette

    Plasmid

    #117120

    Purpose

    Spectinomycin resistance cassette

    Depositor

    Stephan Gruber

    Article

    Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

    Insert

    Spectinomycin resistance cassette for Bacillus subtilis selection
    

    Use

    Golden gate donor vector for gene targeting in ba…

    Available Since

    Nov. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCRISPaint-ProteoTuner DD

    Plasmid

    #67181

    Purpose

    CRISPaint universal donor for fusion tagging allowing inducible destabilization

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Insert

    ProteoTuner DD
    

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDD363

    Plasmid

    #91829

    Purpose

    LoxP-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    LoxP-SEC-LoxP
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223_NKX2-5

    Plasmid

    #221479

    Purpose

    Gateway cloning of NKX2-5 overexpression

    Depositor

    Matthew Meyerson

    Article

    Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

    Insert

    NKX2-5 (NKX2-5 Human)
    

    Use

    Gateway donor vector

    Available Since

    Aug. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. PTPN11 in pENTR

    Plasmid

    #216604

    Purpose

    gateway cloning donor vector containing PTPN11

    Depositor

    Christopher Loewen, Douglas Allan

    Insert

    PTPN11 (PTPN11 Human)
    

    Use

    Gateway entry plasmid

    Available Since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. p2attPC-FO

    Plasmid

    #51551

    Purpose

    DICE donor plasmid with the FOXA2 and OTX2 transcription factors.

    Depositor

    Michele Calos

    Article

    Zhu et al Nucleic Acids Res. 2013 Dec 4.

    Inserts

    FOXA2
    OTX2
    

    Expression

    Mammalian

    Available Since

    March 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAC1817_pCR8-gSMN2-EX

    Plasmid

    #118651

    Purpose

    Transient transfection; Expressed SMN2-EX CasRx gRNA; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    gSMN2-EX
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDD364

    Plasmid

    #91831

    Purpose

    Lox511I-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Lox511I-SEC-Lox511I
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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