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Showing: 321 - 360 of 23042 results

  1. MXS Chaining Kit

    Plasmid Kit
    #1000000064
    Purpose
    MXS-chaining system for assembly of constructs optimized for synthetic biology applications in mammalian systems. 14 different fluorescent proteins, 10 mammalian promoters/enhancers, 3 polyA signals
    Depositor
    Pierre Neveu
    Available Since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  2. CRISPaint Gene Tagging Kit

    Plasmid Kit
    #1000000086
    Purpose
    Modular system for integrating large DNA fragments into defined genomic locations. Canonical NHEJ mechanism. For tagging of endogenous proteins with luciferase, fluorescent proteins, epitope tags
    Depositor
    Veit Hornung
    Available Since
    Sept. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  3. pBad-mAmetrine

    Plasmid
    #18083
    Purpose
    Bacterial expression of mAmetrine fluorescent protein
    Depositor
    Robert Campbell
    Article
    Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1
    Insert
    mAmetrine fluorescent proteins
    Tags
    His
    Expression
    Bacterial
    Mutation
    The fluorescent protein was inserted between XhoI…
    Available Since
    May 29, 2008
    Availability
    Academic Institutions and Nonprofits only

  4. pAAV-hSyn-sbGLuc-B7-EYFP

    Plasmid
    #114110
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, B7 transmembrane region, and enhanced yellow fluorescent protein; control for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Gomez-Ramirez et al J Neurosci Res. 2020 Mar;98(3):471-480.
    Inserts
    Gaussia luciferase, slow burn
    B7 hinge region
    Enhanced Yellow Fluorescent Protein
    Use
    AAV
    Expression
    Mammalian
    Available Since
    March 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    114110-plasmid-map-sequence-id-252520

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  6. pET28a-baPrs-hdNadV

    Plasmid
    #91950
    Purpose
    pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi
    Depositor
    George Cătălin Marinescu
    Article
    Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10
    Inserts
    Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
    Phosphoribosyl Pyrophosphate Synthetases
    Expression
    Bacterial
    Mutation
    changed Leucine 135 to Isoleucine
    Promoter
    T7
    Available Since
    Sept. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    91950-plasmid-map-sequence-id-194125

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  7. pMS22

    Plasmid
    #129710
    Purpose
    Expresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusion
    Depositor
    Alexandra Deaconescu
    Article
    Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.
    Insert
    Escherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)
    Tags
    10-Histidine tag cleavable with PreScission Prote…
    Expression
    Bacterial
    Mutation
    RssB E134G:E135S:E136G:E137S
    Promoter
    T7
    Available Since
    Aug. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    129710-plasmid-map-sequence-id-254924

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  8. pAAV-EFIa-DIO-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid
    #114105
    Purpose
    Cre-dependent fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO3) for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Insert
    sbGLuc-VChR1-EYFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available Since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    114105-plasmid-map-sequence-id-252565

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  15. pAS1NB m Rosella I

    Plasmid
    #71247
    Purpose
    a biosensor comprised of a fast-maturing pH-stable red fluorescent protein fused to a pH-sensitive green fluorescent protein variant. Mitochodrial localization.
    Depositor
    Mark Prescott
    Article
    Rosado et al Autophagy. 2008 Feb;4(2):205-13. Epub 20
    Insert
    Rosella
    Tags
    yeast mitochondrial targeting sequence of citrate…
    Expression
    Yeast
    Promoter
    PGK1
    Available Since
    Nov. 19, 2015
    Availability
    Academic Institutions and Nonprofits only
    71247-plasmid-map-sequence-id-363461

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  16. pcDNA3.1-CAG-sbGLuc-hGtARC2-EYFP

    Plasmid
    #114111
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Guillardia theta anion channelrhodopsin-2, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Prakash et al J Neurosci Res. 2018 Aug 28. doi: 10.100
    Insert
    Gaussia luciferase, slow burn; Guillardia theta anion channelrhodopsin-2, human codon optimized
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    114111-plasmid-map-sequence-id-252509

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  17. pAAV-hSyn-sbGLuc-CheRiff-EGFP

    Plasmid
    #114108
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Ikefuama et al Int J Mol Sci. 2022 Oct 27;23(21):12994.
    Insert
    Gaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    114108-plasmid-map-sequence-id-252563

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  18. pAAV-Hb9-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid
    #114103
    Purpose
    fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein (luminopsin LMO3) for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Petersen et al Front Neurol. 2022 Jan 20;12:792643. doi
    Insert
    sbGLuc-VChR1-EYFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    motoneuron-specific Homeobox 9 promoter
    Available Since
    Aug. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    114103-plasmid-map-sequence-id-252555

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  19. pAAV-hSyn-iLMO (slGLuc-Mac-EYFP)

    Plasmid
    #114100
    Purpose
    fusion protein of Gaussia luciferase variant superluminescent, Leptosphaeria maculans opsin, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11
    Insert
    Gaussia luciferase, superluminescent; Leptosphaeria maculans opsin; EYFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    hSyn
    Available Since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    114100-plasmid-map-sequence-id-252513

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  23. GeneWeld Vectors for Targeted Integration Using CRISPR/Cas9

    Plasmid Kit
    #1000000154
    Purpose
    Vectors for integration using short homology and CRISPR/Cas9 to produce knockout alleles with and without a fluorescent reporter, fusion proteins with endogenous genes, and Cre drivers.
    Depositor
    Jeffrey Essner
    Available Since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only

  39. pCKmatBC

    Plasmid
    #138587
    Purpose
    matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat
    Depositor
    Markus Jeschek
    Article
    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10
    Insert
    bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    matB contains native GUG start codon
    Promoter
    Plac promoter
    Available Since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    138587-plasmid-map-sequence-id-274478

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Showing: 321 - 360 of 23042 results