Addgene

We narrowed to 2,858 results for: eed

Showing: 321 - 360 of 2858 results

Results per page:

Sort results by:

pBS-SKII-3XHA-fCLIP-Hygromycin

Plasmid

#188919

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fCLIP-Hygromycin

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH805_pJH_LldR_Pcon-sfGFP.0TAG

Plasmid

#163736

Purpose

Constitutive expression of green fluorescent protein encoding zero stop codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pWDY1228

Plasmid

#138594

Purpose

Expresses NatL2-R156K in E.coli

Depositor

James Naismith

Article

Song et al Angew Chem Int Ed Engl. 2020 Jan 5. doi:

Insert

phenylacetate-coenzyme A ligase

Use

Tags

6-His

Expression

Mutation

changed Arginine 156 to Lysine

Promoter

T7

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGL1269R

Plasmid

#118994

Purpose

Marker fusion of Bar:mCherry (without start codon)

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Insert

Bar:mCherry

Use

Fungal expression

Tags

Expression

Mutation

Promoter

promoter less

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGL1259

Plasmid

#118996

Purpose

GFP (without start and stop codons) in pFGL1010

Depositor

Naweed Naqvi

Article

Yadav et al MBio. 2019 Jul 30;10(4). pii: mBio.01581

Insert

eGFP

Use

Fungal expression

Tags

Expression

Mutation

Promoter

promoter less

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T-1 dATG1 (1188)

Plasmid

#66918

Purpose

bacterial expression of dATG1

Depositor

Michael Karin, Jun Hee Lee

Article

Kim et al Autophagy. 2013 Aug;9(8):1201-13. doi: 1

Insert

ATG1

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCT-GAL93-DEST

Plasmid

#46339

Depositor

Steven Brown

Article

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Type

Empty backbone

Use

Tags

6XHIS

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only
tout velou (ttv)

Plasmid

#37401

Depositor

Norbert Perrimon

Article

Bellaiche et al Nature. 1998 Jul 2;394(6688):85-8.

Insert

tout-velu (ttv Fly)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pPROEX Aqua

Plasmid

#42889

Depositor

Fabienne Merola

Article

Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

Insert

Aquamarine

Use

Tags

6xHis and TEV cleavage

Expression

Bacterial

Mutation

T65S and H148G

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA_RFP-TEVprotease

Plasmid

#124947

Purpose

Expression of RFP-TEVprotease

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Article

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

RFP-TEVprotease

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU42

Plasmid

#167664

Purpose

ENTRY vector for gRNA2 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU41

Plasmid

#167663

Purpose

ENTRY vector for gRNA1 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU44

Plasmid

#167666

Purpose

ENTRY vector for gRNA4 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU43

Plasmid

#167665

Purpose

ENTRY vector for gRNA3 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU45

Plasmid

#167667

Purpose

ENTRY vector for gRNA5 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU46

Plasmid

#167668

Purpose

ENTRY vector for gRNA6 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU48

Plasmid

#167670

Purpose

ENTRY vector for gRNA8 cloning under pAtU3 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU3

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRU47

Plasmid

#167669

Purpose

ENTRY vector for gRNA7 cloning under pAtU6 promoter

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pAtU6

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pKIR1.0

Plasmid

#86409

Purpose

CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

Depositor

Tetsuya Higashiyama

Article

Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

Insert

human-codon-optimized SpCas9

Use

CRISPR

Tags

FLAG and NLS

Expression

Mutation

Promoter

AtRPS5A

Available since

Feb. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-gCarvi-T2A

Plasmid

#190333

Purpose

Fluorescent reporter for cAMP

Depositor

Naoto Saitoh

Article

Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

Insert

gCarvi

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR SFFVp Phy-mCherry-CAAX

Plasmid

#50839

Purpose

Optogenetic PI3K activation system; membrane component

Depositor

Wendell Lim

Article

Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

Insert

PhyB 1-908

Use

Lentiviral and Synthetic Biology

Tags

mCherry

Expression

Mutation

Promoter

SFFV

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pAquaN1

Plasmid

#42888

Depositor

Fabienne Merola

Article

Erard et al Mol Biosyst. 2013 Feb 2;9(2):258-67. doi

Insert

Aquamarine

Use

Tags

Expression

Mammalian

Mutation

T66S and H149G

Promoter

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGST2-TSG101 (2-145)

Plasmid

#184810

Purpose

Bacterial expression of TSG101 UEV domain (aa 2-145)

Depositor

James Hurley

Article

Im et al Structure. 2010 Nov 10. 18(11):1536-47.

Insert

TSG101 (TSG101 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 2-145 only

Promoter

tac

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH726_pJH_LldR_PlldR-sfGFP.15TAG

Plasmid

#163730

Purpose

Lactate-inducible expression of green fluorescent protein encoding fifteen amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp.15TAG

Use

Tags

Expression

Bacterial

Mutation

gfp with first 15 leucine codons mutated to amber

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJH773_pTech_PlldR-HHRz-supP(opal)

Plasmid

#163732

Purpose

Lactate-inducible expression of supP(opal), preceded by a hammerhead ribozyme

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

hhrz-supP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Syn-DIO-NaChBac-dTomato

Plasmid

#236252

Purpose

Cre dependent NaChBac-TdTomato expression

Depositor

Janelia Viral Tools

Article

Kosse et al Nature. 2024 Dec;636(8041):151-161. doi:

Insert

NaChBac-TdTomato

Use

AAV

Tags

TdTomato

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLX-PVX

Plasmid

#213040

Purpose

PVX vector to express heterologous sequences in plants

Depositor

Jose-Antonio Daros

Article

Uranga et al Hortic Res. 2023 Dec 19;11(1):uhad279. d

Insert

Potato virus X

Use

Tags

Expression

Plant

Mutation

Promoter

Cauliflower mosaic virus 35S

Available since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-2xFLAG-SREBP-2

Plasmid

#26807

Depositor

Timothy Osborne

Article

Toth et al Mol Cell Biol. 2004 Sep . 24(18):8288-30

Insert

Sterol Regulatory Element Binding Protein 2 (SREBF2 Human)

Use

Tags

2x FLAG

Expression

Mammalian

Mutation

L7V, G76S, K395R and I482L

Promoter

CMV

Available since

July 26, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pCGT-Sos Cat

Plasmid

#32923

Depositor

Dafna Bar-Sagi

Article

Boykevisch et al Curr Biol. 2006 Nov 7;16(21):2173-9.

Insert

SOS1 (SOS1 Human)

Use

Tags

T7 epitope

Expression

Mammalian

Mutation

Contains aa 564-1049 (catalytic domain)

Promoter

CMV

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
pSAM_Bt

Plasmid

#112497

Purpose

Transposon mutagenesis vector for Bacteroides thetaiotaomicron. Compatible with Insertion Sequencing (INSeq).

Depositor

Andrew Goodman, Jeffrey Gordon

Article

Goodman et al Cell Host Microbe. 2009 Sep 17;6(3):279-

Inserts

INSeq-adapted mariner transposon
Himar1C9

Use

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-D1ER

Plasmid

#36325

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Insert

D1ER second generation cameleon (calcium sensor) targeted to ER

Use

Tags

KDEL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR iSH-YFP-PIF

Plasmid

#50841

Purpose

Optogenetic PI3K activation system; cytoplasmic component

Depositor

Wendell Lim

Article

Toettcher et al Nat Methods. 2011 Sep 11;8(10):837-9. do

Inserts

p85 iSH2
PIF6 1-100

Use

Lentiviral and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 24, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS-SKII-URA-GAL1-HALO-3xHA

Plasmid

#188937

Purpose

Integration cassette to create N-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

URA-GAL1-HALO-3xHA

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Xiap-Myc

Plasmid

#11833

Depositor

Guy Salvesen

Article

Takahashi et al J Biol Chem. 1998 Apr 3. 273(14):7787-90

Insert

XIAP (XIAP Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-rm_gCarvi-T2A

Plasmid

#190334

Purpose

Ratiometric reporter for cAMP

Depositor

Naoto Saitoh

Article

Kawata et al Proc Natl Acad Sci U S A. 2022 Jul 12;11

Insert

ratiometric gCarvi

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
UAS-Syt::Halo7

Plasmid

#87648

Purpose

For creating drosophila transgenics expressing UAS-Syt::Halo7

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

Syt::Halo7

Use

Tags

Expression

Insect

Mutation

S265G in Syt (please see depositor's commen…

Promoter

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-TPase

Plasmid

#51818

Purpose

For in vitro transcription of Tol2 transposase using T7 Promoter

Depositor

Elly Tanaka

Article

Khattak et al Nat Protoc. 2014 Mar;9(3):529-40. doi: 1

Insert

Tol2 Transposase

Use

Unspecified

Tags

Expression

Mutation

Promoter

T7

Available since

April 10, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB1C3-Lux

Plasmid

#109383

Purpose

Arabinose inducible expression of the Vibrio Fischery Lux operon. Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-LUX operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 16, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLys-M1

Plasmid

#109382

Purpose

Arabinose inducible VioB-mCherry expression (stronger RBS). Used to test burden response in E. coli

Depositor

Tom Ellis

Article

Ceroni et al Nat Methods. 2018 Mar 26. pii: nmeth.463

Insert

pARABAD-VioB-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pSG25-pBAD-tetO-deGFPssrA

Plasmid

#102452

Purpose

Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.

Depositor

Richard Murray

Article

Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

Insert

deGFP

Use

Tags

ssrA

Expression

Bacterial

Mutation

Promoter

pBAD-tetO

Available since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 321 - 360 of 2858 results

Results per page:

Sort results by:

	More than 20 requests
	More than 50 requests
	More than 100 requests