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Showing: 161 - 180 of 19140 results

  3. Desmoplakin II no force control (FL-based)

    Plasmid
    #118724
    Purpose
    The no force control for the FL-based human desmoplakin II tension sensor serves to detect changes in FRET between YPet(short) and mCherry that are tension-independent.
    Depositor
    Carsten Grashoff
    Article
    Price et al Nat Commun. 2018 Dec 11;9(1):5284. doi:
    Insert
    human Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry] (DSP Human)
    Use
    Retroviral
    Tags
    YPet(short)-FL-mCherry
    Expression
    Mammalian
    Mutation
    truncation after aa1353
    Promoter
    CMV
    Available since
    Dec. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    118724-plasmid-map-sequence-id-230471

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  4. ABL1-N-HiBiT kinase domain_v1

    Plasmid
    #207121
    Purpose
    Bacterially expressed HiBiT-ABL1 kinase domain (D233-S500) for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Human Abelson Kinase Domain N-terminal HiBiT, N-terminal 10X His-tag (ABL1 Human)
    Use
    Tags
    HiBiT Tag and His-tag with thrombin site
    Expression
    Bacterial
    Mutation
    Codon optmization for expression in BL21DE3
    Promoter
    T7 Promotor
    Available since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    207121-plasmid-map-sequence-id-422565

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  7. pAAV_hSyn_NES-Kinprola_on-mEGFP_WRPE-SV40

    Plasmid
    #233368
    Purpose
    hSyn1 driven expression of the consitutively active kinase activity recorder positive control Kinprola_on fused to mEGFP for neuronal expression through AAV transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Insert
    NES-Kinprola_on-mEGFP
    Use
    AAV
    Tags
    NES and mEGFP
    Expression
    Mutation
    Promoter
    hSyn1
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233368-plasmid-map-sequence-id-466650

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  8. pAAV_hSyn_NES-Kinprola_PKA_T/A-mEGFP_WRPE-SV40

    Plasmid
    #233367
    Purpose
    hSyn1 driven expression of the inactive PKA activity recorder negative control with T/A mutation Kinprola_PKA fused to mEGFP for neuronal expression through AAV transduction
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Insert
    NES-Kinprola_PKA_T/A-mEGFP
    Use
    AAV
    Tags
    NES and mEGFP
    Expression
    Mutation
    Promoter
    hSyn1
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233367-plasmid-map-sequence-id-466644

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  15. pCDNA5/FRT_CMV_NES-Kinprola_on-mEGFP

    Plasmid
    #233355
    Purpose
    CMV driven expression of the consitutively active kinase activity recorder positive control Kinprola_on in mammalian cell lines fused to mEGFP
    Depositor
    Kai Johnsson
    Article
    Sun et al bioRxiv 2024.09.11.611894
    Insert
    NES-Kinprola_on-mEGFP
    Use
    Tags
    NES and mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    233355-plasmid-map-sequence-id-466631

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Showing: 161 - 180 of 19140 results