We narrowed to 75,792 results for: LAS

1. Equalizer-M plasmid

   Plasmid

   #169731

   Purpose

   Equalizer variant plasmid with medium eGFP output levels and medium gene dosage compensation capability.

   Depositor

   Francois St-Pierre

   Article

   Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

   Insert

   tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   pCMV-tetO2

   Available Since

   June 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSEED-OLLAS:HA

   Plasmid

   #219647

   Purpose

   Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest method

   Depositor

   Markus Affolter

   Article

   Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

   Insert

   OLLAS:HA SEED Cassette
   

   Available Since

   Nov. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLas81-LuxI

   Plasmid

   #73445

   Purpose

   HSL relay device: produces 3O-C6-HSL in response to 3O-C12-HSL

   Depositor

   Jim Haseloff

   Article

   Grant et al Mol Syst Biol. 2016 Jan 25;12(1):849.

   Insert

   LuxI
   

   Use

   Synthetic Biology

   Promoter

   pLas81

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-subtilase A

   Plasmid

   #35948

   Depositor

   Zhaoyang Feng

   Article

   Yuan et al PLoS One. 2011;6(8):e22354. Epub 2011 Au

   Insert

   Subtilase A (subA E. Coli)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 14, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pINS-Blast

   Plasmid

   #31388

   Depositor

   Yegor Vassetzky

   Article

   Dmitriev et al BMC Res Notes. 2008 Dec 23;1:135. doi: 1

   Insert

   bsd
   

   Use

   Cre/Lox

   Available Since

   Sept. 13, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sender plasmid

   Plasmid

   #127677

   Purpose

   Encodes the synthase LuxI for the quorum-sensing molecule C6-HSL.

   Depositor

   Friedrich Simmel

   Article

   Schwarz-Schilling et al Integr Biol (Camb). 2016 Apr 18;8(4):564

   Insert

   LuxI
   

   Use

   Synthetic Biology

   Tags

   LVA

   Expression

   Bacterial

   Promoter

   pT7

   Available Since

   Sept. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiMutB3GNT5-blast

   Plasmid

   #208380

   Purpose

   lentiviral vector for expressing human B3GNT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   Beta-1,3-N-Acetylglucosaminyltransferase 5 (B3GNT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   nt 922 c->g (PAM site inactivation)

   Promoter

   EF-1 alpha core promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiMutB3GALT5-blast

   Plasmid

   #208381

   Purpose

   lentiviral vector for expressing human B3GALT5 with C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   nucleotide change in PAM targeting sequence nt 51…

   Promoter

   EF-1 alpha core promoter

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. lentiMutB3GALT5catdead-blast

   Plasmid

   #220869

   Purpose

   lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   encodes D156A and D243A mutations; also, nucleoti…

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-Blast_hCOL4A5-HA

    Plasmid

    #198103

    Purpose

    lentiviral expression of hCOL4A5 in mammalian cells

    Depositor

    Jeffrey Miner

    Insert

    COL4A5 (COL4A5 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    EF1a

    Available Since

    March 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCfuNca-Plastid

    Plasmid

    #190818

    Purpose

    Plastid targeting GFP with ble resistant gene for zeocin

    Depositor

    Peter Kroth

    Article

    Sprecher et al Algal Research. 2023 May; Vol 72: 103136

    Inserts

    Diatom plastid targeted GFP
    Bleomycin resistance gene
    

    Expression

    Bacterial

    Promoter

    Cylindrotheca fusiformis fcp and Nitzschia captiv…

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pWZL Blast ST

    Plasmid

    #13805

    Depositor

    William Hahn

    Article

    Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

    Insert

    SV40 small T antigen (SV40gp7 simian virus)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Feb. 22, 2007

    Availability

    Academic Institutions and Nonprofits only

13. CMV+ plasmid

    Plasmid

    #169740

    Purpose

    Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels. This plasmid has additional sequences in the UTRs to increase gene expression levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    pCMV-tetO2

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. OSC_Reporter_UAS_traffic jam_BoxB_d2eGFP_t2a_Blast

    Plasmid

    #128010

    Purpose

    traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites

    Depositor

    Julius Brennecke

    Article

    Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

    Insert

    traffic jam enhancer driven GFP_P2A-Blasticidin-resistance harboring 10 intronic boxB sites and 14 upstream UAS sites
    

    Use

    Drosophila osc reporter construct

    Available Since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. mc2 blast

    Plasmid

    #206217

    Purpose

    For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

    Depositor

    Simon Conn, Brett Stringer

    Article

    Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Available Since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1+-pHlash

    Plasmid

    #61552

    Purpose

    Expression of pHlash in mammalian cells

    Depositor

    Carl Johnson

    Article

    Zhang et al PLoS One. 2012;7(8):e43072. doi: 10.1371

    Insert

    pHlash
    

    Tags

    NA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

17. mCerulean3-Plastin-C-10

    Plasmid

    #55438

    Purpose

    Localization: Fimbrin/Cytoskeleton, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Plastin (PLS1 Human)
    

    Tags

    mCerulean3

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. CelTag Plasmid

    Plasmid

    #66562

    Purpose

    Encodes CelTag for the tagging and high affinity purification of endogenous yeast proteins.

    Depositor

    David Engelke

    Article

    Carrick et al G3 (Bethesda). 2015 Dec 29. pii: g3.115.

    Insert

    CelTag
    

    Expression

    Bacterial

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. mEmerald-LASP1-C-10

    Plasmid

    #54141

    Purpose

    Localization: Focal Adhesions, Excitation: 487, Emission: 509

    Depositor

    Michael Davidson

    Insert

    LASP1 (LASP1 Human)
    

    Tags

    mEmerald

    Expression

    Mammalian

    Mutation

    A209V

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

20. CMV plasmid

    Plasmid

    #169739

    Purpose

    Unregulated "open-loop" CMV promoter plasmid. This plasmid encodes eGFP to report circuit output levels.

    Depositor

    Francois St-Pierre

    Article

    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:

    Insert

    eGFP
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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