We narrowed to 16,162 results for: grna

1. EC2_3_dCas9_Mxi1_sgRNA

   Plasmid

   #163708

   Purpose

   Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

   Depositor

   Yvonne Nygård

   Article

   Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

   Inserts

   dCas9
   Mxi1+SV40 NLS
   

   Expression

   Yeast

   Mutation

   Point mutations D10A and H840A

   Promoter

   ScTEF1

   Available Since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. SpCas9_sgRNA_expression_in_pBluescript

   Plasmid

   #122089

   Purpose

   U6 driven SpCas9 sgRNA expression vector for cloning own guides

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   SpCas9 tracr (NEWENTRY Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. LCV2_mCherry_AAVS1_sgRNA_2

   Plasmid

   #155105

   Purpose

   lentiviral plasmid expressing mCherry, Cas9 and a gRNA targeting the AAVS1 safe harbor locus

   Depositor

   Jason Moffat

   Article

   Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

   Insert

   AAVS1_sgRNA_2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6 promoter

   Available Since

   Aug. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. p425_Cas9_gRNA_LEU_1014a

   Plasmid

   #87407

   Purpose

   p425_Cas9_gRNA-ARS1014a All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, LEU2 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1014a sequence TTATGTGCGTATTGCTTTCA in yeast chromosome 10.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1014a
   

   Use

   CRISPR

   Promoter

   ADH1, pTyrosine

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9_U6_sgRNA_Mettl3C

   Plasmid

   #165420

   Purpose

   sgRNA construct for targeting Mettl3 C-terminus

   Depositor

   Neil Brockdorff

   Article

   Wei et al Genome Res. 2021 Aug;31(8):1395-1408. do

   Insert

   PspCas9 gRNA targeting Mettl3 C-terminus (Mettl3 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. px458_2A_GFP_sgRNA_TIAL1

   Plasmid

   #106096

   Purpose

   CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting TIAL1

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

   Insert

   gRNA TIAL1
   

   Use

   CRISPR

   Available Since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. ATP1A1_G3_Dual_sgRNA

   Plasmid

   #173202

   Purpose

   Coselection for PE3 or HDR in human cells. Vector for tandem expression of ATP1A1 G3 sgRNA with a user-specified PE3 nick sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   ATP1A1 G3 sgRNA + user-specified sgRNA
   

   Use

   CRISPR; Prime editing

   Expression

   Mammalian

   Promoter

   Tandem U6 promoters

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. gRNAy

   Plasmid

   #168294

   Purpose

   Expresses gRNA targeting the yellow gene in Drosophila

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Oct;4(5):739-751. doi: 10

   Insert

   U6.3-gRNA[y]-scaffold
   

   Expression

   Insect

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. px458_2A_GFP_sgRNA_G3BP1_G1

   Plasmid

   #127114

   Depositor

   Thomas Tuschl

   Article

   Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

   Insert

   gRNA G3BP1 (G3BP1 Human)
   

   Use

   CRISPR

   Available Since

   July 31, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pT7sgRNA

    Plasmid

    #111820

    Purpose

    Expresses sgRNA in T. brucei cells

    Depositor

    David Horn

    Article

    Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

    Insert

    sgRNA
    

    Use

    CRISPR

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBP_sgRNA

    Plasmid

    #190128

    Purpose

    Level 0 MoClo plasmid for Golden Gate cloning of sgRNAs

    Depositor

    Veronica Godoy-Carter

    Article

    Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pT7EGFPgRNA

    Plasmid

    #46760

    Depositor

    Wenbiao Chen

    Article

    Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

    Insert

    egfp target
    

    Use

    CRISPR

    Promoter

    T7

    Available Since

    Aug. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. ATP1A1_G2_Dual_sgRNA

    Plasmid

    #173201

    Purpose

    Coselection for ABE or NHEJ in human cells. Vector for tandem expression of ATP1A1 G2 sgRNA with a user-specified sgRNA from two independent U6 promoters. Cloning of oligos using BbsI sites.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G2 sgRNA + user-specified sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. B52_puro_empty_gRNA

    Plasmid

    #197557

    Purpose

    sites for cloning two gRNA with puromycin cassette

    Depositor

    Darius Bägli

    Type

    Empty backbone

    Use

    Empty grna backbone with puromycin resistance gene

    Expression

    Mammalian

    Promoter

    U6 for gRNA, CMV for puromycin resistance.

    Available Since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMC0x_gRNA_Pos5_Ptet

    Plasmid

    #179336

    Purpose

    gRNA Position Vector 5, used to create multi gRNA NT-CRISPR plasmid with pST_119_LVL2 cam

    Depositor

    Anke Becker

    Article

    Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

    Insert

    Ptet gRNA with sfGFP dropout
    

    Use

    CRISPR

    Available Since

    March 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pUC_sgRNA

    Plasmid

    #68710

    Purpose

    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.

    Depositor

    Roberto Docampo

    Article

    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Available Since

    Sept. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. sgRNA.SFFV.GFP

    Plasmid

    #169938

    Purpose

    Expresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and GFP from SFFV promoter as the marker for infection. 3rd generation lentiviral backbone.

    Depositor

    Benjamin Ebert

    Type

    Empty backbone

    Use

    Lentiviral

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. ATP1A1_G4_Q118R_v2_Dual_epegRNA_tevopreQ1

    Plasmid

    #187456

    Purpose

    Coselection for prime editing in human cells. Vector for tandem expression of ATP1A1 Q118R-G4_v2 optimized epegRNA with a user-specified epegRNA. pU6-tevopreq1-GG-acceptor-like plasmid

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    ATP1A1 G4 Q118R_v2 epegRNA (tevopreQ1) (ATP1A1 Human)
    

    Use

    CRISPR; Prime editing

    Expression

    Mammalian

    Promoter

    Tandem U6 promoters

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHpgRNA13

    Plasmid

    #160127

    Purpose

    To express a sgRNA with constant targeting ADE2 by RNA pol II promoter PTEF1

    Depositor

    Yongjin Zhou

    Article

    Gao et al iScience. 2021 Feb 9;24(3):102168. doi:

    Insert

    PTEF1-HH-gRNA-ADE2- HDV-TAMO
    

    Expression

    Yeast

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLCKO_AAVS1_sgRNA_2

    Plasmid

    #155085

    Purpose

    lentiviral plasmid expressing Cas9 gRNA targeting AAVS1 safe harbor locus

    Depositor

    Jason Moffat

    Article

    Aregger et al Nat Metab. 2020 Jun;2(6):499-513. doi: 1

    Insert

    AAVS1_sgRNA_2
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Aug. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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