We narrowed to 3,100 results for: rho

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pTriEx-RhoA-wt_mScarlet-i_SGFP2

Plasmid

#85071

Purpose

Green/red FRET biosensor to monitor RhoA nucleotide loading state and activity

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor wildtype with mScarlet-i and SGFP2 (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1-RhoA G17A

Plasmid

#69357

Purpose

expression of nucleotide free RhoA in bacterial cells

Depositor

Rafael Garcia-Mata

Article

Garcia-Mata et al Methods Enzymol. 2006;406:425-37.

Insert

RhoA (RHOA Human)

Tags

GST

Expression

Bacterial

Mutation

G17A

Promoter

tac

Available Since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only
pTriEx-RhoA-Q63L_mScarlet-i_SGFP2

Plasmid

#85072

Purpose

Green/red FRET biosensor for RhoA in the GTP locked constitutively active state

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

RhoA Biosensor constitutively active (Q63L) (RHOA Human)

Tags

6xHis and mScarlet-i and SGFP2

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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delCMV-mCherry-2x-Rhotekin-GBD

Plasmid

#214142

Purpose

Rho activity sensor

Depositor

Leif Dehmelt

Article

Nanda et al Nat Commun. 2023 Dec 15;14(1):8356. doi:

Insert

3xRhotekin-GBD (Rtkn Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

Only the GTPase binding domain (GBD) is included

Promoter

delCMV

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Rho-EGFP-ETSQVAPA

Plasmid

#45399

Depositor

David Williams

Article

Trivedi et al J Neurosci. 2012 Aug 1;32(31):10587-93.

Insert

Rhodopsin (RHO Bovine)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 16, 2013

Availability

Academic Institutions and Nonprofits only
pGEX-4T3-RhoA-Q63L

Plasmid

#12961

Depositor

Gary Bokoch

Insert

RhoA constitutively active (RHOA Human)

Tags

GST

Expression

Bacterial

Mutation

Q63L Constitutively active

Available Since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only
pB-Tet-On-Arl13b-RhoA sensor

Plasmid

#189613

Purpose

Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); Arl13b-fused with a sGFP-mScarlet-I based sensor

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

Arl13b

Tags

GFP and mScarlet

Expression

Mammalian

Promoter

Tet3G

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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RhoC FLARE.sc mCer, mVenus - wt

Plasmid

#65071

Purpose

RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus

Promoter

CMV, p10

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only
pB-Tet-On-HTR6-RhoA sensor

Plasmid

#189614

Purpose

Tet-On cilia-targeted RhoA sensor (Xlone piggybac back-bone); HTR6-fused with a sGFP-mScarlet-I based sensor

Depositor

David Clapham

Article

Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

Insert

HTR6 (HTR6 Human)

Tags

GFP and mScarlet

Expression

Mammalian

Promoter

Tet3G

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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RhoC FLARE.sc mCer, mVenus - CA

Plasmid

#65424

Purpose

RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus, Q63L in RhoC

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
RhoC FLARE.sc mCer, mVenus - DN

Plasmid

#65423

Purpose

RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

Depositor

Klaus Hahn

Article

Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

Insert

RBD, mCerulean, mVenus, RhoC

Expression

Bacterial, Insect, and Mamm…

Mutation

T153M in mVenus, T19N in RhoC

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only
CLPIT Cry2PHR-mCh-RhoA

Plasmid

#42959

Depositor

David Schaffer

Article

Bugaj et al Nat Methods. 2013 Feb 3. doi: 10.1038/nm

Insert

Cry2PHR-mCherry-RhoA (RHOA Human, Mustard Weed)

Use

Retroviral

Expression

Mammalian

Promoter

Tet-Off promoter

Available Since

April 9, 2013

Availability

Academic Institutions and Nonprofits only

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pCR-BluntII-TOPO-RhoC

Plasmid

#14099

Depositor

Richard Hynes

Article

Clark et al Nature. 2000 Aug 3. 406(6795):532-5.

Insert

Human RhoC (RHOC Human)

Expression

Bacterial

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pTriEX-Antenna-GDI RhoA

Plasmid

#189669

Purpose

Expression of Antenna-RhoA

Depositor

Klaus Hahn

Article

Hodgson et al Nat Chem Biol. 2016 Aug 8. doi: 10.1038/

Insert

RhoA (RHOA Human)

Expression

Mammalian

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pTriex-mVenus-PI-RhoA(Q63L)

Plasmid

#86978

Purpose

PI(DM): Photo-activatable/light sensitive

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
RhoA (RHOA Human)

Tags

6xHis and mVenus

Expression

Bacterial, Insect, and Mamm…

Mutation

Q63L

Promoter

CMV

Available Since

Feb. 24, 2017

Availability

Academic Institutions and Nonprofits only

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H2A-mTurquoise-RHOG-QL-deltaCAAX

Plasmid

#176096

Purpose

Constitutively active, nuclear localized RhoG

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RHOG (RHOG Human)

Expression

Mammalian

Mutation

QL

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5-Flag-RhoA K6,7R

Plasmid

#11751

Depositor

Jeff Wrana

Article

Ozdamar et al Science. 2005 Mar 11. 307(5715):1603-9.

Insert

RhoA (RHOA Human)

Tags

Flag

Expression

Mammalian

Mutation

K6,7R

Available Since

Aug. 4, 2006

Availability

Industry, Academic Institutions, and Nonprofits
RCAS(A) dRhoA V14 (CT#846)

Plasmid

#13949

Depositor

Cliff Tabin

Article

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

RhoA (Rho1 Fly)

Use

Retroviral; Avian expression

Mutation

G14V mutation

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only
pX459-HypaCas9-mR2-RHOA_sgRNA

Plasmid

#183877

Purpose

pX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.

Depositor

Alisa Piekny

Article

Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

Insert

RHOA sgRNA spacer (RHOA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

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RCAS(A) dRhoA N19 (CT#847)

Plasmid

#14002

Depositor

Cliff Tabin

Article

Hartmann et al Cell. 2001 Feb 9. 104(3):341-51.

Insert

RhoA (Rho1 Fly)

Use

Retroviral; Avian expression

Mutation

T19N

Available Since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only