We narrowed to 7,521 results for: Cit

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pJYDN2p

Plasmid

#162455

Purpose

Positive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).

Depositor

Gideon Schreiber

Article

Zahradník et al bioRxiv Preprint 2020

Insert

Aga2p-DnbALFA (AGA2 )

Use

Shuttle vector bacteria/yeast

Tags

DnbALFA nanobody and HA and myc

Promoter

GAL1

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pET-His-VCre

Plasmid

#123131

Purpose

Bacterial expression plasmid for wild-type VCre with an N-terminal His-tag.

Depositor

David Liu

Article

Bessen et al Nat Commun. 2019 Apr 26;10(1):1937. doi:

Insert

VCre recombinase

Use

Cre/Lox

Tags

6x His tag

Expression

Bacterial

Promoter

T7

Available Since

April 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-HsSENP3(307-574)

Plasmid

#71481

Purpose

Encodes the catalytic domain of human SENP3. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 3 (SENP3 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Deleted amino acids 1-306 (catalytic domain only)

Promoter

T7

Available Since

Dec. 11, 2015

Availability

Academic Institutions and Nonprofits only
mCardinal-H3-23

Plasmid

#56163

Purpose

Localization: Nucleus/Histones, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

H3 (H3C2 Human)

Tags

mCardinal

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3flag-RanGAPdeltaN

Plasmid

#25104

Depositor

Guy Salvesen

Article

Mikolajczyk et al J Biol Chem. 2007 Sep 7. 282(36):26217-2

Insert

RanGAP1 deleted N-term domain (RANGAP1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Deleted amino acids 1-203.

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 3xF-NHP2

Plasmid

#126871

Purpose

Expresses 3xFlag NHP2

Depositor

Kathleen Collins

Article

Egan et al Mol Cell Biol. 2010 Jun;30(11):2775-86.

Insert

NHP2 (NHP2 Human)

Tags

3xFlag

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shIFNAR

Plasmid

#127699

Purpose

Doxycyclin inducible shRNA knockdown of mouse IFNAR gene

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shIFNAR1 Mmu (Ifnar1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-EWSR1::WT1(-KTS)

Plasmid

#220565

Purpose

Lentiviral expression of human EWSR1::WT1(-KTS)

Depositor

Sean Lee

Article

Magrath et al Commun Biol. 2024 Apr 4;7(1):411. doi: 1

Insert

EWSR1::WT1(-KTS) (EWSR1, WT1 Human)

Use

Lentiviral

Tags

HA

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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p323.hSyn.Halo-mFMRP.Wpre

Plasmid

#185909

Purpose

Expresses FMRP fused to HaloTag for protein localization through fluorescence imaging

Depositor

Young J Yoon

Article

Monday et al Neuron. 2022 Aug 17;110(16):2588-2606.e6

Insert

HaloTag fused to mouse Fragile X mental retardation protein (Ctsa Mouse)

Use

Lentiviral

Tags

HaloTag

Promoter

hSyn (human Synapsin promoter)

Available Since

Oct. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pRGEN-CMV-VPR-L1-CjCas9 V-D

Plasmid

#169913

Purpose

Expression of a VPR-dCjCas9 fusion protein to activate gene expression.

Depositor

Zhili Rong

Article

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

dCjCas9

Use

CRISPR

Tags

2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)

Expression

Mammalian

Mutation

D8A, H559A in cjCas9

Promoter

CMV

Available Since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMG059 MBP-CREB-His

Plasmid

#154076

Purpose

Expresses MBP-CREB-His (human CREB as a fusion protein with MBP and His- tags) in E.coli

Depositor

Jesse Zalatan

Article

Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

Insert

CREB1 (CREB1 Human)

Tags

His6 and MBP

Expression

Bacterial

Promoter

Tac

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-ASPM CTR

Plasmid

#27669

Depositor

Joseph Loturco

Article

Paramasivam et al Cell Cycle. 2007 Jul 1. 6(13):1605-12.

Insert

Abnormal spindle-like microcephaly associated (Aspm Rat)

Tags

GFP

Expression

Mammalian

Mutation

Contains carboxy terminal region of ASPM (amino a…

Available Since

March 21, 2011

Availability

Academic Institutions and Nonprofits only
pUC19 PKC epsilon

Plasmid

#8442

Depositor

Frederic Mushinski

Article

Mischak et al J Biol Chem 1993 Mar 25;268(9):6090-6.

Insert

Mouse Protein Kinase C epsilon (Prkce Mouse)

Expression

Bacterial

Available Since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pEF Doc2beta WT GFP

Plasmid

#128820

Purpose

To visualize Doc2beta WT in PC12 cells

Depositor

Edwin Chapman

Article

Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

Insert

Doc2beta (Doc2b Rat)

Tags

EGFP

Expression

Mammalian

Promoter

EF-1alpha

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Cathepsin S

Plasmid

#84694

Purpose

for expression of Cathepsin S in Leishmania tarantolae

Depositor

Hans Brandstetter

Article

Freier et al Sci Rep. 2015 Aug 3;5:12707. doi: 10.103

Insert

cathepsin S (CTSS Human)

Use

Expression in leishmania tarantolae

Tags

his-tag

Promoter

unknown

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB405

Plasmid

#68670

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMpGWB406

Plasmid

#68671

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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AIRAP 11: PSMD14.pEYFP.V1

Plasmid

#21801

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

PSMD14 (Psmd14 Mouse)

Tags

YFP

Expression

Mammalian

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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AAV hSYN DIO mGluR1-myc-FLAG

Plasmid

#166227

Purpose

AAV expressing cre-dependent mGluR1-myc-FLAG

Depositor

Jason Aoto

Article

Cai et al Neuron. 2021 Apr 7;109(7):1137-1149.e5.

Insert

metabotropic glutamate receptor 1 (Grm1 Mouse)

Use

AAV and Cre/Lox

Tags

Myc and FLAG

Expression

Mammalian

Promoter

human Synapsin

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pUltra-Exon1Q23-Myc-B

Plasmid

#110488

Purpose

Lentiviral vector to express wild-type N terminal HTT gene with partial long 3'UTR in mammalian cells (encodes Myc-tagged wild-type huntingtin exon1 fragment with 23 repeats of Q)

Depositor

Baoji Xu

Article

Xu et al PLoS One. 2017 May 11;12(5):e0177610. do

Insert

Homo sapiens huntingtin (HTT Human)

Use

Lentiviral

Tags

Myc

Expression

Mammalian

Mutation

short 3'UTR mutant huntingtin exon1 fragment…

Promoter

UbC

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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Pvalb-2A-Dre Flp-in replacement vector

Plasmid

#61571

Purpose

Recombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Pvalb-2A-Dre (Pvalb Mouse, D6 Bacteriophage)

Use

Recombinase-mediated cassette exchange using flp …

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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GST-TEV-FUS-1-413aa

Plasmid

#29632

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS-1-413aa (FUS Human)

Tags

GST and TEV

Expression

Bacterial

Mutation

Contains FUS aa# 1-413

Available Since

July 18, 2011

Availability

Academic Institutions and Nonprofits only
CMV-mEGFP(A206K)-paCaMKII-ShadowG

Plasmid

#167475

Purpose

Expresses paCaMKII in mammalian cells to measure structural change of paCaMKII.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

mEGFP(A206K)-paCaMKII-ShadowG

Tags

ShadowG and mEGFP(A206K)

Expression

Mammalian

Promoter

CMV

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pGWKS5

Plasmid

#139412

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Citrine marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

Citrine

Expression

Yeast

Promoter

TEF1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLC-HA-Nedd8-P2A-Hygro

Plasmid

#124306

Purpose

Lentiviral vector for expression of HA tagged Nedd8-P2A-Hygro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

Nedd8 (NEDD8 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-FRT-FLEX-GtACR2-EYFP

Plasmid

#114369

Purpose

expresses Flpo recombinase-dependent GtACR2

Depositor

Mingshan Xue

Article

Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

Insert

GtACR2-EYFP (NEWENTRY )

Use

AAV

Tags

EYFP

Expression

Mammalian

Promoter

Ef1a

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pHis-hMTMR1

Plasmid

#100723

Purpose

Expression of human MTMR1 PH-PTP domain

Depositor

Byung Il Lee

Article

Bong et al PLoS One. 2016 Mar 28;11(3):e0152611. do

Insert

MTMR1 (MTMR1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains amino acids 95-607

Promoter

T7

Available Since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pET28a-HsSENP7

Plasmid

#71470

Purpose

Encodes human full length SENP7. Suitable for in vitro transcription/translation (T7).

Depositor

Jorge Eduardo Azevedo

Article

Mendes et al Biochim Biophys Acta. 2015 Oct 29. pii:

Insert

SUMO1/sentrin specific peptidase 7 (SENP7 Human)

Tags

Hexahistidine

Expression

Bacterial

Mutation

Gene optimised for E. coli expression (Genscript)

Promoter

T7

Available Since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only
pUAST mito roGFP2-Grx1

Plasmid

#64995

Purpose

for expression of mitochondrial roGFP2-Grx1 with the Gal4 expression system in drosophila

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Glutaredoxin-1 (GLRX Human)

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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FusionRed-CD36-C-10

Plasmid

#56104

Purpose

Localization: Membrane, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

CD36 (CD36 Human)

Tags

FusionRed

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits