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Halo-GPI-RUSH

Plasmid

#166903

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-Halo-GPI

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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mApple-TNFa-RUSH

Plasmid

#166902

Purpose

RUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-mApple

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

mApple

Expression

Mammalian

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

Plasmid

#214995

Purpose

Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (EEA1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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GST-OPTN

Plasmid

#171424

Purpose

For the purifcation of OPTN protein

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

OPTN (OPTN Human)

Expression

Bacterial

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

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pVH607-Tier1-PCREm4-Nluc

Plasmid

#169594

Purpose

Tier-1 vector encoding PCREm4-driven Nluc expression (PCREm4-Nluc-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

cAMP response element-controlled NanoLuc Luciferase expression cassette

Expression

Mammalian

Promoter

PCREm4

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV Fcgamma RIIa IRES neo

Plasmid

#14948

Depositor

Axel Nohturfft

Article

Trivedi et al Proc Natl Acad Sci U S A. 2006 Nov 28. 1

Insert

Fcgamma RIIa (FCGR2A Human)

Expression

Mammalian

Available Since

May 21, 2007

Availability

Academic Institutions and Nonprofits only

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pLV_Stargazin-mTurquoise2-iLID

Plasmid

#161001

Purpose

Mammalian expression of mTurquoise2-tagged iLID anchored to the plasma membrane via N-terminal fusion to Stargazin for slow diffusion

Depositor

Sean R Collins

Article

Natwick et al ACS Synth Biol. 2021 May 21;10(5):1009-1

Insert

Stargazin-mTurquoise2-iLID

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLPC-NFLAG TRF1

Plasmid

#16058

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF1 (TERF1 Human)

Use

Retroviral

Tags

Flag

Mutation

aa2-439

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Fos WT

Plasmid

#8966

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Oct. 4, 2005

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_class_I_2

Plasmid

#164987

Purpose

Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA class I

Use

CRISPR

Expression

Mammalian

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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EpsilonCOP EGFP

Plasmid

#128151

Purpose

Expression of epsilon COP

Depositor

Jennifer Lippincott-Schwartz

Article

Ward et al J Cell Biol. 2001 Nov 12;155(4):557-70.

Insert

epsilon COP (COPE Bovine)

Expression

Mammalian

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Flag-HA-BRCC3

Plasmid

#22540

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

BRCC3 (BRCC3 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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pGL3-U6-sgRNA-BFP

Plasmid

#107722

Purpose

For expression of sgRNA from the U6 promoter with BFP expression simultaneously

Depositor

Xingxu Huang

Article

Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

Type

Empty backbone

Use

CRISPR

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pSF-CjPglB

Plasmid

#199305

Purpose

pSN18 derivative encoding C.jejuni PglB with C-terminal FLAG epitope tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

CjPglB with flag tag

Tags

Flag tag

Expression

Bacterial

Promoter

pBAD

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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CHOP::GFP

Plasmid

#21898

Depositor

David Ron

Article

Novoa et al J Cell Biol. 2001 May 28. 153(5):1011-22

Insert

CHOP (Ddit3 Mouse)

Use

Non-viral

Tags

EGFP

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pPRIME-CMV-LNGFR-recipient

Plasmid

#11660

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with CMV promoter controlling low-affinity nerve growth factor receptor (LNGFR) expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

LNGFR

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Cerulean-Epac2-Venus

Plasmid

#138196

Purpose

FRET-based biosensor for monitoring Epac2 activation.

Depositor

Jin Zhang

Article

Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

Insert

Cerulean-Epac2-Venus (Rapgef4 Mouse, Synthetic)

Tags

Cerulean and Venus

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nls-st1dCas9-3nls-3XGFP-2nls

Plasmid

#64113

Purpose

Expression of St1 dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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HLA_DRB1_07_01

Plasmid

#165014

Purpose

Expression of HLA-DRB1*07:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-DRB1*07:01 (HLA-DRB1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAGM38869

Plasmid

#153220

Purpose

Level 0 Arabidopsis U6-promoter vector

Depositor

Sylvestre Marillonnet

Article

Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

Insert

Arabidopsis U6 snRNA promoter

Use

Synthetic Biology

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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