We narrowed to 17,230 results for: EMB

1. Guide-Generating Vector

   Plasmid

   #125752

   Purpose

   Guide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assembly

   Depositor

   Rodrigo Ledesma-Amaro

   Article

   McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

   Insert

   Cys4-GFPdropout-SpCas9scaffold
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. EB3-eGFP-2xC2-iLID

   Plasmid

   #114419

   Purpose

   anchor iLID domain to plasma membrane with slower diffusion

   Depositor

   Dorus Gadella

   Article

   Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

   Insert

   iLID domain
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. GGE209

   Plasmid

   #120793

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   InsUp-Mfe
   

   Use

   Cloning/assembly plasmid

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCG-B-mSpyCatcher-C

   Plasmid

   #87374

   Purpose

   An ORF part-containing vector harbouring the minimal SpyCatcher (ΔN1ΔC22) sequence with 4 bp overhangs specific for the ‘upstream SpyPart’ position within an ORF assembly

   Depositor

   Tom Ellis

   Article

   Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

   Insert

   mSpyCatcher
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCG-A-celA(SP)-B

   Plasmid

   #87371

   Purpose

   An ORF part-containing vector harbouring the signal peptide from CelA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

   Depositor

   Tom Ellis

   Article

   Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

   Insert

   CelA signal peptide
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCG-A-xynA(SP)-B

   Plasmid

   #87372

   Purpose

   An ORF part-containing vector harbouring the signal peptide from XynA with 4 bp overhangs specific for the ‘signal peptide’ position within an ORF assembly

   Depositor

   Tom Ellis

   Article

   Gilbert et al ACS Synth Biol. 2017 Mar 7. doi: 10.1021

   Insert

   XynA signal peptide
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7T-CcadTSMod

   Plasmid

   #99874

   Purpose

   RNA synthesis for microinjection into xenopus laevis embryo

   Depositor

   Marc Tramier

   Article

   Herbomel et al Sci Rep. 2017 Mar 22;7:45058. doi: 10.10

   Insert

   CcadTSMod
   

   Use

   Xenopus microinjection

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQuantA-hygro-amp

   Plasmid

   #45584

   Depositor

   Andrzej Dziembowski

   Article

   Orlowska et al Nucleic Acids Res. 2013 Sep;41(17):e167.

   Type

   Empty backbone

   Use

   Cre/Lox; Dt40 cell line targeting

   Tags

   Quant-A tag

   Expression

   Mutation

   Promoter

   Available since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTH646-2µ-RLuc

   Plasmid

   #40600

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Insert

   Renilla Luciferase
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Dec. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGBW-m4046268

    Plasmid

    #145423

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pTEF1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pGBW-m4046216

    Plasmid

    #145490

    Purpose

    Yeast Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    

    Use

    Tags

    Cleavable thrombin;6xHIS

    Expression

    Yeast

    Mutation

    Saccharomyces cerevisiae recode 1

    Promoter

    pGAL1

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. GGE094

    Plasmid

    #120787

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsDown_Zeta-SfiI
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. GGE091

    Plasmid

    #120786

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for integration into Y. lipolytica genome, randomly or at zeta platforms. Linearization of cassette by SfiI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsUp_Zeta-SfiI
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. GGE254

    Plasmid

    #120789

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsDown_Lip-NotI
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. GGE255

    Plasmid

    #120788

    Purpose

    Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Sequence for homologous integration into Y. lipolytica genome. Linearization of cassette by NotI restriction enzyme after the assembly

    Depositor

    Tristan Rossignol

    Article

    Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

    Insert

    InsUp_Lip-NotI
    

    Use

    Cloning/assembly plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR Bmi1-C

    Plasmid

    #30511

    Depositor

    John Gurdon

    Article

    Pasque et al EMBO J. 2011 May 6;30(12):2373-87. doi:

    Insert

    Bmi1 (Bmi1 Mouse)
    

    Use

    Gateway - donor vector

    Tags

    Expression

    Mutation

    NO STOP

    Promoter

    Available since

    June 25, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXF262

    Plasmid

    #139038

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    cox-4 (no STOP codon) (W09C5.8 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSDH50

    Plasmid

    #139037

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    tomm-20 (1-55 a.a.) (no STOP codon) (tomm-20 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJF7

    Plasmid

    #139036

    Purpose

    MosSCI SapTrap assembly: Cassette 2 for 4- or 5-cassette system

    Depositor

    Erik Griffin

    Article

    Fan et al G3 (Bethesda). 2019 Dec 17. pii: g3.119.

    Insert

    tomm-20 (no STOP codon) (tomm-20 Nematode)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTH736-CEN-staHIS3

    Plasmid

    #38228

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Insert

    HIS3 (HIS3 Budding Yeast, Synthetic)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    TDH3 (=GPD)

    Available since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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