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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 1781 - 1800 of 2873 results

  7. pJK347

    Plasmid
    #71833
    Purpose
    Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59D), and purK genes
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    changes histidine-59 to aspartate
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71833-plasmid-map-sequence-id-131050

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  8. pJK348

    Plasmid
    #71832
    Purpose
    Cloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59N), and purK genes
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Inserts
    hypothetical protein
    N5-carboxyaminoimidazole ribonucleotide mutase
    N5-carboxyaminoimidazole ribonucleotide synthetase
    Expression
    Bacterial
    Mutation
    changes histidine-59 to asparagine
    Available Since
    Jan. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    71832-plasmid-map-sequence-id-131051

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  9. pHL 1410

    Plasmid
    #53011
    Purpose
    pLtetO-1:DsrA, pCon:ompC::T7noRBS(900bps), pLlacO-1:rpoS::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    DsrA sRNA
    ompC
    rpoS
    Use
    Synthetic Biology
    Tags
    T7noRBS (900bp) and gfp
    Expression
    Bacterial
    Mutation
    contains −150 to +30 bp relative to start codon a…
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    Jan. 27, 2015
    Availability
    Academic Institutions and Nonprofits only

  10. pHL 1035

    Plasmid
    #52999
    Purpose
    pLtetO-1:RhyB, pCon:fhlA::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    fhlA
    sodB
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    contains −309 to +96 bp relative to start codon a…
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pHL 1033

    Plasmid
    #52998
    Purpose
    pLtetO-1:RhyB, pCon:rpoS::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    RhyB sRNA
    rpoS
    sodB
    Use
    Synthetic Biology
    Tags
    gfp and mCherry
    Expression
    Bacterial
    Mutation
    contains −150 and +30 bp relative to start codon …
    Promoter
    pCon, pLlacO-1, and pLtetO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  12. pHL 1073

    Plasmid
    #53003
    Purpose
    pCon:ompC::mCherry, pLlacO-1:sodB::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    mCherry
    GFP
    Use
    Synthetic Biology
    Tags
    ompC (target mRNA) and sodB (target mRNA)
    Expression
    Bacterial
    Mutation
    contains −56 to +141 bp relative to start codon a…
    Promoter
    pCon and pLlacO-1
    Available Since
    May 16, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. pHL 1303

    Plasmid
    #53008
    Purpose
    pLtetO-1:sodB::mCherry, pLlacO-1:ompC::gfp
    Depositor
    Han Lim
    Article
    Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10
    Inserts
    sodB
    ompC
    Use
    Synthetic Biology
    Tags
    GFP and mCherry
    Expression
    Bacterial
    Mutation
    contains -56 to +141 relative to start codon and …
    Promoter
    pLlacO-1 and pLtetO-1
    Available Since
    May 14, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. CROP-seq-opti

    Plasmid
    #106280
    Purpose
    A version of the CROP-seq plasmid as presented in Datlinger et al. that contains the sgRNA-(F+E)-combined optimized backbone for CRISPRi from Chen et al.
    Depositor
    Jay Shendure
    Article
    Hill et al Nat Methods. 2018 Feb 19. pii: nmeth.460
    Insert
    EF1a-Puro-WPRE-hU6-gRNA
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    March 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    106280-plasmid-map-sequence-id-354474

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  16. pLVX-mCherry-SPASTIN

    Plasmid
    #180647
    Purpose
    Lentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.
    Depositor
    Wesley Sundquist
    Article
    Wenzel et al Elife. 2022 Sep 15;11:e77779. doi: 10.75
    Insert
    SPASTIN
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Starts at M87, has siRNA resistance to GAACAGUGUG…
    Available Since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    180647-plasmid-map-sequence-id-360020

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  17. pDIRECT_22C

    Plasmid
    #91135
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers, Plant Selection: 2x35S:npt II
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available Since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    91135-plasmid-map-sequence-id-182261

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Showing: 1781 - 1800 of 2873 results