Addgene

We narrowed to 31,164 results for: uros

Showing: 1781 - 1800 of 31164 results

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pLenti-BE3RA-PGK-Puro

Plasmid

#110843

Purpose

Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Tags

Expression

Mutation

D10A

Promoter

EF1s

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe(puro) mouse Egr4

Plasmid

#108105

Purpose

Retroviral vector for expression of mouse Egr4

Depositor

Peter Johnson

Article

Salotti et al Mol Cell Biol. 2015 Mar;35(5):866-83. do

Insert

Egr4 (Egr4 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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OCT4 GFP puro Donor 2

Plasmid

#22210

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

SA-OCT-GFP-2APuro-PA (POU5F1 Human)

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-mTurquoise2-Nuf2

Plasmid

#80761

Purpose

Express Nuf2 N-terminally labeled with mTurquoise2

Depositor

Daniel Needleman

Article

Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

Insert

Nuf2 (NUF2 Human)

Use

Retroviral

Tags

mTurquoise2

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-puro-eGFP-HEC1-9A

Plasmid

#114023

Purpose

Inducible expression of eGFP-tagged HEC1-9A in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1 - 9A (NDC80) (NDC80 Human)

Use

Tags

Expression

Mammalian

Mutation

Hec1 - 9A

Promoter

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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tet-pLKO shMKP1.v2 puro

Plasmid

#87791

Purpose

Expresses an inducible short hairpin targeting human MKP1 sequence

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

shMKP1 (DUSP1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TRE tight

Available since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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pMKO.1 puro-luc (shRNA)

Plasmid

#153962

Purpose

Expresses a shRNA against firefly luciferase

Depositor

Hiroyuki Konishi

Article

Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

Insert

shRNA against firefly luciferase (19-bp-long target)

Use

Luciferase, RNAi, and Retroviral

Tags

N/A

Expression

Mammalian

Mutation

Promoter

U6 promoter

Available since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y112N-pBabe-puro

Plasmid

#19238

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tyrosin 112 to Asparagin

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-5xE

Plasmid

#91993

Purpose

Expresses FLAG-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S828E, T830E, S831E, S834E (5xE)

Promoter

Available since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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HA-VHL Y112H-pBabe-puro

Plasmid

#19237

Depositor

William Kaelin

Article

Li et al Mol Cell Biol. 2007 Aug . 27(15):5381-92

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

changed Tyrosin 112 to Histidin

Promoter

Available since

Oct. 1, 2008

Availability

Academic Institutions and Nonprofits only

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lentiGuide-Puro-Chen-tracrRNA_2xBsmBI

Plasmid

#196710

Purpose

Backbone for cloning single guides (Chen tracrRNA PMID: 24360272) or guide libraries with untranscribed barcodes and a tracrRNA of choice

Depositor

Bernhard Schmierer

Article

Schmierer et al Mol Syst Biol. 2017 Oct 9;13(10):945. do

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pIRESpuro-Flag-Pol b(K72A)

Plasmid

#23258

Depositor

Robert Sobol

Article

Tang et al Mol Cancer Res. 2010 Jan 12. ():.

Insert

DNA polymerase beta (K72A) (POLB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

K72A

Promoter

Available since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
pALPS puro miR30-STAT1

Plasmid

#117160

Purpose

Target site ATATCCAGTTCCTTTAGGGCC

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

shRNA targeting miR30

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLV(shRNA)-Puro-U6-CCEPR

Plasmid

#108277

Purpose

shRNA for CCEPR lncRNA

Depositor

Karl Munger

Article

Sharma et al Virology. 2018 Feb 7;518:8-13. doi: 10.1

Insert

CCEPR (CCEPR Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

April 3, 2018

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Nprl2-g1)-PGKpuroBFP-W

Plasmid

#105037

Purpose

Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nprl2 (Nprl2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(Nprl2-g2)-PGKpuroBFP-W

Plasmid

#105038

Purpose

Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

Depositor

Kosuke Yusa

Article

Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

Insert

Nprl2 (Nprl2 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pMMP-puro-Flag-HA-NR6A1

Plasmid

#46833

Depositor

Phil Sharp

Article

Gurtan et al Genes Dev. 2013 Apr 15;27(8):941-54. doi

Insert

NR6A1 (Nr6a1 Mouse)

Use

Retroviral

Tags

Flag-HA, plus 8-amino acid residue linker

Expression

Mammalian

Mutation

None

Promoter

Available since

Nov. 25, 2013

Availability

Academic Institutions and Nonprofits only
pMSCV-puro-TTF-1-HDD

Plasmid

#74991

Purpose

Retroviral vector holding human TTF-1 (NKX2-1) with the homeodomain deleted

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

TTF-1 HDD mutant (NKX2-1 Human)

Use

Retroviral

Tags

None

Expression

Mammalian

Mutation

HDD = homeodomain deleted

Promoter

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

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pLKO1-puro-U6-sgRNA-TGM2

Plasmid

#69239

Purpose

U6 driven SpCas9 sgRNA expression for TGM2 site

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

TGM2 sgRNA (TGM2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pMXs-puro HA-Atg14*dCC

Plasmid

#38266

Depositor

Noboru Mizushima

Article

Itakura et al Mol Biol Cell. 2008 Dec . 19(12):5360-72

Insert

autophagy related genes 14 (ATG14 Human)

Use

Retroviral

Tags

3xHA

Expression

Mammalian

Mutation

changed A618T, A621T, C624A and T627C for siRNA r…

Promoter

LTR

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only