Addgene

We narrowed to 2,124 results for: AP1

Showing: 1801 - 1820 of 2124 results

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pGEX-2T FRB Y2105A

Plasmid

#68726

Purpose

Point mutation Tyr 2105 → Ala in the FKBP12-rapamycin binding (FRB) domain of mTOR

Depositor

Jie Chen

Article

Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

Insert

FKBP12-rapamycin binding (FRB) domain of MTOR (MTOR Human)

Use

Tags

GST-tagged

Expression

Bacterial

Mutation

FRB domain only aa2015-2114; Y2105A

Promoter

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1-C/pIRES puro Glue (H987-end)

Plasmid

#73458

Purpose

Express human C-terminal part (H987-end) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1-C/pIRES puro Glue (S778-Q986)

Plasmid

#73457

Purpose

Express human C-terminal part (S778-Q986) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
hPlekhm1-Mid/pIRES puro Glue (Q513-E784)

Plasmid

#73455

Purpose

Express human middle part (Q513-E784) Plekhm1 in mammalian cells

Depositor

Paul Odgren

Article

Witwicka et al PLoS One. 2015 May 19;10(5):e0127537. do

Insert

Plekhm1 (PLEKHM1 Human)

Use

Tags

CBP tag, HA tag, and strep tag

Expression

Mammalian

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only
pPD95.75-cYAP-1 S104A

Plasmid

#66858

Purpose

Generate transgenic C.elegans with cYAP1- fused to GFP, in which Serine 104 is mutated to alanine

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

YAP-1 (yap-1 Nematode)

Use

Tags

GFP

Expression

Worm

Mutation

Serine104 is mutated to alanine

Promoter

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
KHBD00399

Plasmid

#34509

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG2702 (Pbp95 Fly)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-Flag-YAP-H122L

Plasmid

#33086

Depositor

Kunliang Guan

Article

Zhao et al Genes Dev. 2007 Nov 1. 21(21):2747-61.

Insert

YAP (YAP1 )

Use

Tags

Flag

Expression

Mammalian

Mutation

H122L

Promoter

CMV

Available since

March 21, 2012

Availability

Academic Institutions and Nonprofits only
H75R SUMO1

Plasmid

#221060

Purpose

Expression of H75R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H75R site mutation on SUMO 1 (SUMO1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

H75R

Promoter

T7

Available since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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polySUMO1

Plasmid

#221066

Purpose

Expression of polySUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

Five repeats of SUMO1 each separated by (GGS)4 (SUMO1 Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

Promoter

tac

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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E67K SUMO1

Plasmid

#221065

Purpose

Expression of E67K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

E67K site mutation on SUMO 1 (SUMO1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

E67K

Promoter

T7

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H35K SUMO1

Plasmid

#221055

Purpose

Expression of H35K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H35K site mutation on SUMO 1 (SUMO1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

H35K

Promoter

T7

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H35S SUMO1

Plasmid

#221056

Purpose

Expression of H35S SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H35S site mutation on SUMO 1 (SUMO1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

H35S

Promoter

T7

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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E67R SUMO1

Plasmid

#221064

Purpose

Expression of E67R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

E67R site mutation on SUMO 1 (SUMO1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

E67R

Promoter

T7

Available since

Jan. 24, 2025

Availability

Academic Institutions and Nonprofits only

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H43R SUMO1

Plasmid

#221057

Purpose

Expression of H43R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H43R site mutation on SUMO 1 (SUMO1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

H43R

Promoter

T7

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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H35R SUMO1

Plasmid

#221054

Purpose

Expression of H35R SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H35R site mutation on SUMO 1 (SUMO1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

H35R

Promoter

T7

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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H43K SUMO1

Plasmid

#221058

Purpose

Expression of H43K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H43K site mutation on SUMO 1 (SUMO1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

H43K

Promoter

T7

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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H43S SUMO1

Plasmid

#221059

Purpose

Expression of H43S SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H43S site mutation on SUMO 1 (SUMO1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

H43S

Promoter

T7

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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H75K SUMO1

Plasmid

#221061

Purpose

Expression of H75K SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H75K site mutation on SUMO 1 (SUMO1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

H75K

Promoter

T7

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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H75S SUMO1

Plasmid

#221062

Purpose

Expression of H75S SUMO1 protein under T7 promoter

Depositor

Michael Rosen

Article

Kim et al J Am Chem Soc. 2024 Feb 7;146(5):3383-33

Insert

H75S site mutation on SUMO 1 (SUMO1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

H75S

Promoter

T7

Available since

Jan. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-acatenin

Plasmid

#186458

Purpose

Expresses human alpha-catenin with an N-terminal GST tag and a PRescission Protease cleavage site

Depositor

Tina Izard

Article

Rangarajan et al Commun Biol. 2023 Mar 16;6(1):276. doi:

Insert

CTNNA1 (CTNNA1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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