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pEF5/FRT/DEST-3xHA/mGli3/Flag P1-6A

Plasmid

#51248

Purpose

Encodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907,980,1006 mutated to Ala

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli3 (Gli3 Mouse)

Use

Flpin system

Tags

3xHA-TEV and Flag

Expression

Mammalian

Mutation

amino acids 849,865,877,907,980,1006 mutated to A…

Promoter

EF1a

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only
Wnt3A STOP

Plasmid

#35871

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt3A (WNT3A Human)

Use

Gateway Cloning

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAPK15

Plasmid

#23697

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAPK15 (MAPK15 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
IMS-HyPer2

Plasmid

#60248

Purpose

Genetically-encoded fluorescent hydrogen peroxide indicator HyPer2 targeted to mitochondrial intermembrane spase.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

IMS-HyPer2

Tags

Mitochondrial intermembrane space targeting signa…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

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GBSGFP insulator

Plasmid

#121975

Purpose

Gli activity reporter, mouse transgenesis

Depositor

James Briscoe

Article

Balaskas et al Cell. 2012 Jan 20;148(1-2):273-84. doi:

Insert

GBS(long)-GFP-insulator (Gnb5 Mouse)

Use

Mouse transgenesis

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only
pMH4-SYN-EGFP-ER

Plasmid

#22285

Depositor

Thomas Oertner

Article

Holbro et al Proc Natl Acad Sci U S A. 2009 Sep 1;106

Insert

EGFP-ER

Tags

EGFP-ER

Expression

Mammalian

Mutation

EGFP alanine 206 changed to Lysin

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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pGFH-9

Plasmid

#39538

Depositor

Katharina Strub

Article

Mary et al RNA. 2010 May;16(5):969-79. Epub 2010 Ma

Insert

SRP9 (SRP9 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2012

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4L S468A

Plasmid

#27037

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Tags

HA

Expression

Mammalian

Mutation

S468A

Available Since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only
pCMV7.1_3xFLAG-LATS2

Plasmid

#172987

Purpose

constitutive expression of FLAG-tagged LATS2

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-LATS2 (LATS2 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pLck-mVenus-C1

Plasmid

#84337

Purpose

Expresses a mVenus tagged with a membrane localization sequence

Depositor

Dorus Gadella

Article

van Unen et al BMC Cell Biol. 2018 Jun 7;19(1):6. doi:

Insert

Lck (MGCVCSSNPELPVAT)

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 12, 2016

Availability

Academic Institutions and Nonprofits only

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Wnt1 STOP

Plasmid

#35868

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt1 (WNT1 Human)

Use

Gateway Cloning

Mutation

None

Available Since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLKO.1-mPRAS40

Plasmid

#15480

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

PRAS40 shRNA (Akt1s1 Mouse)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pEX-CMV-SP-YFP-STM1(24-685) EF hand 3 mut (A79K, N80D, D82G)

Plasmid

#18865

Depositor

Tobias Meyer

Article

Brandman et al Cell. 2007 Dec 28. 131(7):1327-39.

Insert

Stromal interaction molecule 1 (STIM1 Human)

Tags

YFP

Expression

Mammalian

Mutation

3 mutations (A79K, N80D, D82G) in the EF hand of …

Available Since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pK263.CAG-loxP-stop-loxP-NLSturboRFP-ires-tTA-WPRE

Plasmid

#89587

Purpose

For nucleus-specific turboRFP labeling in single cells, pK263 should be used with pK031.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

nuclear localization signal (nls) turboRFP

Expression

Mammalian

Available Since

June 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCR3-FLAG-Gephyrin P1

Plasmid

#68816

Purpose

Flag tagged Geph mammalian expression

Depositor

Shiva Tyagarajan

Article

Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

Insert

Gephyrin (Gphn Rat)

Tags

3xFLAG

Expression

Mammalian

Mutation

silent mutations on internal EcoRI site. Gephyrin…

Promoter

CMV

Available Since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pYFP-NGAT(A193T,N194Y)

Plasmid

#11388

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1 Human)

Tags

YFP

Expression

Mammalian

Mutation

Changed Alanine 193 to Threonine, and Asparagine …

Available Since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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pNES-iRFP-C1

Plasmid

#116863

Purpose

cytosolic iRFP

Depositor

Gerry Hammond

Article

Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

Insert

iRFP713

Tags

X.leavis map2k1.L(32-44)

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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pG5ElbLuc

Plasmid

#89341

Purpose

The plasmid pG5ElbLuc was prepared from pG5E1bCAT by replacing the CAT gene (EcoRI and Sty I fragment) with the firefly luciferase gene (from pGEM-Luc) as a 1745bp StuI-Hind111 fragment.

Depositor

Roger Davis

Article

Seth et al J Biol Chem. 1992 Dec 5;267(34):24796-80

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available Since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-PKAcat-mCherry

Plasmid

#181841

Purpose

mCherry-tagged PKA catalytic subunit for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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HA-MCPIP1

Plasmid

#127459

Purpose

Expressing human MCPIP1 in mammalian cells

Depositor

Mingui Fu

Article

Liang et al J Exp Med. 2010 Dec 20;207(13):2959-73.

Insert

MCPIP1 (ZC3H12A Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

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