We narrowed to 7,176 results for: cas9 plasmid

1. pMLS281

   Plasmid

   #73732

   Purpose

   SapTrap syntron-embedded FLP-on N-terminal connector donor plasmid

   Depositor

   Erik Jorgensen

   Article

   Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

   Insert

   syntron embedded N-terminal FLP-on
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWT007f

   Plasmid

   #107891

   Purpose

   W1.0.3 plasmid. Contains PTetO-sd2U-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.3

   Depositor

   David Liu

   Article

   Tang et al Science. 2018 Feb 15. pii: science.aap89

   Insert

   PTetO-sd2U-Cas9, PLac-sgRNA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA

   Plasmid

   #222861

   Purpose

   This plasmid codes for the OgeuIscB nickase

   Depositor

   Vincent Dion

   Article

   Mangin et al bioRxiv 2023.10.24.563270

   Insert

   OgeuIscB Nickase
   

   Use

   CRISPR

   Tags

   3X HA and Nucleoplasmin NLS and ITR2

   Expression

   Mammalian

   Mutation

   E193A for nickase mutation

   Promoter

   CMV

   Available since

   Oct. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWT007a

   Plasmid

   #107889

   Purpose

   W1.0.1 plasmid. Contains PTetO-SD8-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.1

   Depositor

   David Liu

   Article

   Tang et al Science. 2018 Feb 15. pii: science.aap89

   Insert

   PTetO-SD8-Cas9, PLac-sgRNA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pWT007g

   Plasmid

   #107890

   Purpose

   W1.0.2 plasmid. Contains PTetO-sd2-Cas9, PLac-sgRNA1 and is part of CAMERA 1.0.2

   Depositor

   David Liu

   Article

   Tang et al Science. 2018 Feb 15. pii: science.aap89

   Insert

   PTetO-sd2-Cas9, PLac-sgRNA1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX458_mCherry-NPM1-gRNA for HCT116

   Plasmid

   #238249

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to NPM1 locus

   Depositor

   Denes Hnisz

   Insert

   NPM1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX458_mCherry-NPM1-gRNA for U2OS

   Plasmid

   #238250

   Purpose

   Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to NPM1 locus

   Depositor

   Denes Hnisz

   Insert

   NPM1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pML104-KanMx4

   Plasmid

   #83476

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

   Depositor

   Patricia Wittkopp

   Insert

   KanMX4
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pML104-HygMx4

   Plasmid

   #83475

   Purpose

   Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.

   Depositor

   Patricia Wittkopp

   Insert

   HphMX4
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET-MBP-NLS-Geo_st

    Plasmid

    #87703

    Purpose

    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP and SV40 NLS

    Depositor

    Jennifer Doudna

    Insert

    GeoCas9
    

    Use

    Tags

    10xHis-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pML104-NatMx3

    Plasmid

    #83477

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains NatMX3 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    NatMx3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

12. pSBtet-RB-PE2

    Plasmid

    #173903

    Purpose

    SB-transposon with inducible expression of SpCas9 PE2

    Depositor

    Ronald Cohn

    Insert

    PE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pML107-KAE1

    Plasmid

    #232891

    Purpose

    Plasmid expressing Cas9 and gRNA GATGACAACTGAATGCAGAG which targets the KAE1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    KAE1 gRNA (KAE1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pML107-SAM50

    Plasmid

    #232895

    Purpose

    Plasmid expressing Cas9 and gRNA AATAGTTTATGTAAGAACAG which targets the SAM50 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    SAM50 gRNA (SAM50 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    April 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pML107-ARB1

    Plasmid

    #232888

    Purpose

    Plasmid expressing Cas9 and gRNA CAAAAACTAACTGCTTACGG which targets the ARB1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    ARB1 gRNA (ARB1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pML107-RER2

    Plasmid

    #232884

    Purpose

    Plasmid expressing Cas9 and gRNA AGAATCGCATCTCTACACGG which targets the RER2 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    RER2 gRNA (RER2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pML104-LEU2-3

    Plasmid

    #232894

    Purpose

    Plasmid expressing Cas9 and gRNA GTTCTTAACTAGGATCATGG which targets the RER2 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    LEU2 gRNA (RER2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pML104-HygMx4-URA3-3

    Plasmid

    #232886

    Purpose

    Plasmid expressing Cas9 and gRNA GAAGTAACAAAGGAACCTAG which targets the URA3 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    URA3 gRNA (URA3 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pML104-HygMx4-URA3-2

    Plasmid

    #232885

    Purpose

    Plasmid expressing Cas9 and gRNA TCGTACCACCAAGGAATTAC which targets the URA3 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    URA3 gRNA (URA3 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pML107-AVO1

    Plasmid

    #232889

    Purpose

    Plasmid expressing Cas9 and gRNA AATGATGACGACCATACCAG which targets the AVO1 gene.

    Depositor

    Jolanda Van Leeuwen

    Insert

    AVO1 gRNA (AVO1 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    snR52

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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