We narrowed to 55,886 results for: plasmid

1. pX458-Ef1a-dCas9-VPR-H2B-GFP (CRISPRa)

   Plasmid

   #175571

   Purpose

   Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX458-Ef1a-dCas9-VPR-H2B-mCherry (CRISPRa)

   Plasmid

   #175572

   Purpose

   Inactivated Cas9 (dCas9) fusion to VPR for the purpose of targeted activation

   Depositor

   Joseph Brzezinski

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. BirA(R118G)-HA destination vector

   Plasmid

   #53581

   Purpose

   To fuse your protein of interest to the N-terminus of BirA(R118G) and use in proximity-dependent biotin identification (BioID)

   Depositor

   Karl Kramer

   Type

   Empty backbone

   Tags

   BirA(R118G) and HA tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH

   Plasmid

   #100050

   Purpose

   AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by CaMKIIa promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Has Service

   AAV9

   Insert

   hChR2(E123T/H134R)
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   humanized, E123T/H134R

   Promoter

   CaMKIIa

   Available Since

   Jan. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pN3-Nipbl

   Plasmid

   #107716

   Purpose

   Expression plasmid for 3xFLAG-tagged NIPBL

   Depositor

   Guntram Suske

   Insert

   NIPBL (Nipbl Mouse)
   

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCEV-G2-Km ymNeongreen

   Plasmid

   #193959

   Purpose

   TEF1 promoter controlled expression plasmid with G418 resistance for expressing ymNeongreen in budding yeast

   Depositor

   Bas Teusink

   Insert

   ymNeonGreen
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-CamKIIa-jGCaMP8f-WPRE

   Plasmid

   #176750

   Purpose

   AAV-mediated expression of GCaMP8f under the CamKIIa promoter

   Depositor

   Loren Looger

   Has Service

   AAV Retrograde, AAV1, and AAV5

   Insert

   jGCaMP8f
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   CamKIIalpha

   Available Since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

   Plasmid

   #100834

   Purpose

   AAV expression of ultrasensitive protein calcium sensor from CamKII promoter

   Depositor

   James M. Wilson

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   GCaMP6f
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

   Promoter

   CamKII

   Available Since

   March 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGBW-m4134906

   Plasmid

   #151960

   Purpose

   Mammalian expression plasmid for Human coronavirus OC43 nucleocapsid

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   Human coronavirus OC43 nucleocapsid protein (EYW02_gp8 Human coronavirus OC43)
   

   Expression

   Mammalian

   Mutation

   wt

   Promoter

   CMV

   Available Since

   May 20, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. PSD-95/GW1

    Plasmid

    #197332

    Purpose

    Plasmid expressing an antigen targeting PSD-95 MAGUK

    Depositor

    Morgan Sheng

    Insert

    PSD95 (DLG4 Human)
    

    Expression

    Mammalian

    Available Since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGBW-m4134902

    Plasmid

    #151912

    Purpose

    Mammalian expression plasmid for Human coronavirus 229E nucleocapsid

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    Human coronavirus 229E nucleocapsid protein (N Human coronavirus 229E)
    

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. pGBW-m4134908

    Plasmid

    #151957

    Purpose

    Mammalian expression plasmid for Human coronavirus NL63 nucleocapsid

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    Human coronavirus NL63 nucleocapsid protein (HCNV63gp6 Human coronavirus NL63)
    

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    CMV

    Available Since

    May 20, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pT7TS-rich

    Plasmid

    #99050

    Purpose

    This plasmid is useful for in vitro transcription of genes using T7 promoter. Can be used for Xenopus oocyte assay. It is derived from pT7TS. More sites for cloning are incorporated.

    Depositor

    Richard Bélanger

    Type

    Empty backbone

    Use

    Unspecified; Xenopus oocyte system

    Promoter

    pT7

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3-hTICAM1-VenusFlag

    Plasmid

    #197632

    Purpose

    Human TRIF protein expression in mammalian cells

    Depositor

    Haian Fu

    Insert

    TICAM1 (TICAM1 Human)
    

    Tags

    Venus-Flag

    Expression

    Mammalian

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. AnkyrinB-GFP/pEGFP-N1

    Plasmid

    #197327

    Purpose

    Plasmid expressing an antigen targeting AnkyrinB

    Depositor

    Vann Bennett

    Insert

    AnkyrinB (Ank2 Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-CaMKIIa-hChR2(H134R)-mCherry

    Plasmid

    #26975

    Purpose

    AAV expression of humanized ChR2(H134R) fused to mCherry driven by CaMKIIa promoter for optogenetic excitation

    Depositor

    Karl Deisseroth

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    hChR2(H134R)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    H134R

    Promoter

    CaMKIIa

    Available Since

    Feb. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pML104-HygMx4

    Plasmid

    #83475

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains HphMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    HphMX4
    

    Expression

    Yeast

    Available Since

    Oct. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-EF1a-jGCaMP8m-WPRE

    Plasmid

    #176757

    Purpose

    AAV-mediated expression of GCaMP8m under the EF1a promoter

    Depositor

    Loren Looger

    Has Service

    AAV Retrograde, AAV1, and AAV9

    Insert

    jGCaMP8m
    

    Use

    AAV

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL1180-HR-PUbECFP

    Plasmid

    #47917

    Purpose

    Donor plasmid for homologous recombination that expresses ECFP consitutively using the Aedes aegypti polyubiquitin (PUb) promoter.

    Depositor

    Leslie Vosshall

    Insert

    ECFP
    

    Tags

    NLS

    Expression

    Insect

    Promoter

    Aedes aegypti polyubiquitin (PUb)

    Available Since

    Sept. 26, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1(-) FMC-63 scFv-Fc (huFc)

    Plasmid

    #183252

    Purpose

    Soluble expression of the murine anti-human CD19 scFv-Fc (clone FMC-63). Human IgG1 Fc.

    Depositor

    Alexander McLellan

    Insert

    anti-CD19 scFvFc (FMC-63)
    

    Expression

    Mammalian

    Available Since

    Oct. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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