We narrowed to 2,171 results for: Endog
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Plasmid#121475PurposeCEN TRP1 CNA1ΔAIDDepositorInsertCNA1deltaAID
UseYeast cen vectorMutationTruncated after amino acid 508PromoterEndogenous genomic promoter regionAvailable SinceApril 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTJK519
Plasmid#121476PurposeCEN TRP1 CNA1ΔCBDDepositorInsertCNA1deltaCBD
UseYeast cen vectorMutationTruncated after amino acid 453PromoterEndogenous genomic promoter regionAvailable SinceMarch 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pRPL28 SpHIS5 (pNTI663)
Plasmid#115433PurposeExpresses wild-type RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationMfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(Q38P) SpHIS5 (pNTI664)
Plasmid#115434PurposeExpresses Q38P RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationQ38P; MfeI site in intronPromoterAshbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRPL28(H39L) SpHIS5 (pNTI665)
Plasmid#115435PurposeExpresses H39L RPL28 from HIS3 yeast plasmidDepositorInsertsExpressionYeastMutationH39L; MfeI site in intronPromoterashbya gospii TEF1 and endogenousAvailable SinceOct. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRS315Nab3D191stop
Plasmid#110537PurposeExpresses yeast NAB3 using the endogenous promoterDepositorAvailable SinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
GTU-y
Plasmid#81095PurposeGene Tagging vector URA/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)DepositorInsertmCitrine
UseCre/LoxTagsmCitrineExpressionYeastMutationMutated mCitrine for monomeric isoform (A206K L22…Available SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTU-ir
Plasmid#81097PurposeGene Tagging vector URA/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a URA selection marker, originating from pSIVu (ID:81089)DepositorInserttdiRFP
UseCre/LoxTagstdiRFPExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTL-c
Plasmid#81098PurposeGene Tagging vector LEU/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)DepositorInsertyemCFP
UseCre/LoxTagsyemCFPExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
GTT-c
Plasmid#81108PurposeGene Tagging vector TRP/CFP - Plasmid for yeast enhanced monomeric CFP (yemCFP) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)DepositorInsertyemCFP
UseCre/LoxTagsyemCFPExpressionYeastAvailable SinceNov. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
miR-451 Trap
Plasmid#61487Purposeto knockdown endogenous miR144 levels; lentiviral vector containing 8x copies of bulged trap sequences cloned 3' to GFP under the control of a biPGK promoterDepositorInsertmiR-451 Trap
UseLentiviralTagsGFPExpressionMammalianPromoterbidirectional phosphoglycerate kinase promoter (b…Available SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
HR-TERT-promotor
Plasmid#71398PurposeHomologues recombination donor plasmid fo the wild type TERT promotor region. Includes 1kb of TERT coding region in right homologues arm.DepositorInsertTERT (TERT Human)
PromoterEndogenous TERT promotorAvailable SinceDec. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC-BETA
Plasmid#53272PurposeContains crtE, crtB, crtI, and crtY carotenoid pathway genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces beta-carotene in Escherichia coliDepositorInsertcrtE, crtY, crtI, crtB
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable SinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-ATG8(416)/GFP-AUT7(416)
Plasmid#49425PurposeExpresses ATG8 fused at the N terminus to GFP under the control of the endogenous promoter in the pRS416 vector.DepositorInsertautophagy-related 8 (ATG8 Budding Yeast)
TagsGFPExpressionBacterial and YeastPromoterATG8Available SinceJan. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
Nanog-CreER targeting construct
Plasmid#59720PurposeCre-ER knockin targeting construct for mouse endogenous Nanog locusDepositorInsertcreERT2
UseMouse TargetingPromotermNanogAvailable SinceJan. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
GAPDH-CPG-TV
Plasmid#70148Purposea targeting vector, to target the endogenous Gapdh CPG island with Snrp-GFP-polyADepositorInsert5'hGapdh-Snrp-GFP-polyA-PGK-PURO-3'hGapdh
UseMouse Targeting and Synthetic BiologyAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAVS1_Puro_PGK1_3xFLAG_Twin_Strep
Plasmid#68375PurposeDoyon lab Tandem-Affinity Purification Following (i) Nuclease-Driven Gene Addition to the AAVS1 Genomic Safe Harbor Locus and (ii) Endogenous TAP Tagging.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCRISPR and TALEN; ZfnTags3xFLAG_Twin_StrepExpressionMammalianPromoterhuman PGK1Available SinceOct. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR
Plasmid#126705Purposedonor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequenceDepositorInsertGFP
UseCRISPRAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-TetON-dual-SunTag-Hygro
Plasmid#121119PurposeEndogenous gene activation with the SunTag CRISPR activation systemDepositorInsertdCas9-GCN4x10_scFv-VP64
UseCRISPRExpressionMammalianPromoterTet-ONAvailable SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only