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Showing: 1841 - 1860 of 1954 results

  2. pCDNA3.1-SP-ALFA-lk-HiBiT-lk-ADGRL3-L928A-M929A

    Plasmid
    #233065
    Purpose
    Expresses tethered-agonist dead mouse ADGRL3-L928A-M929A (UniProt Q80TS3-3). An N-terminal AFLA-tag (SRLEEELRRRLTE) and HiBiT tag (VSGWRLFKKIS) follow the endogenous ADGRL3 signal peptide.
    Depositor
    Jonathan Javitch
    Article
    Perry-Hauser et al J Biol Chem. 2025 Jan 9:108174. doi: 10.
    Insert
    ADGRL3 (Adgrl3 Mouse)
    Use
    Tags
    ALFA and HiBiT
    Expression
    Mammalian
    Mutation
    Leucine 928 to Alanine; Methionine 929 to Alanine
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233065-plasmid-map-sequence-id-464575

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  3. pUC19-POLBHR-eGFP

    Plasmid
    #176064
    Purpose
    Homology region +/- 800bp to the transcription start site of POLB, with EGFP inserted in-frame on the N-terminus of POLB, and a mutation in the PAM site used by POLBKO gRNA1 in POLB exon1
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    EGFP-PolB (POLB Human)
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    pUC with endogenous SapI mutated out, PAM mutatio…
    Promoter
    N/A
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    176064-plasmid-map-sequence-id-352734

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  4. CPH3568 (YCp LEU2 Rpb1 P1455_E1456InsGGGGGLEVLFQGP(H)10,N1501K; msLink1)

    Plasmid
    #91819
    Purpose
    Yeast expression of S. cerevisiae Rpb1 with an internal PreScission protease site and 10xHis tag for purification of the linker-CTD
    Depositor
    Chris Hill
    Article
    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1
    Insert
    RPO21 (RPO21 Budding Yeast)
    Use
    Tags
    10xHis
    Expression
    Yeast
    Mutation
    5xGly linker, PreScission Protease site (LEVLFQGP…
    Promoter
    Endogenous Rpb1 promoter
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    91819-plasmid-map-sequence-id-176494

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  7. loxP-G418-loxP-3xALFA-Halo-Rab11 HR

    Plasmid
    #229676
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    Halo, 3xALFA
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229676-plasmid-map-sequence-id-457076

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  8. loxP-Puro-loxP-3xV5-SNAP-Rab11 HR

    Plasmid
    #229677
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    SNAP, 3xV5
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229677-plasmid-map-sequence-id-457129

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  10. AP1muA-mStayGold-polyA-Hygro HR

    Plasmid
    #229679
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    monomeric StayGold
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229679-plasmid-map-sequence-id-457128

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  11. loxP-Hygro-loxP-HA-mStayGold-Rab11 HR

    Plasmid
    #229678
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with monomeric StayGold and an HA epitope tag. Contains a hygromycin resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    monomeric StayGold, HA
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229678-plasmid-map-sequence-id-457125

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  12. AP1muA-Halo-ALFA-polyA-G418 HR

    Plasmid
    #229681
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    Halo, ALFA
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229681-plasmid-map-sequence-id-457127

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  13. AP1muA-SNAP-V5-polyA-Puro HR

    Plasmid
    #229680
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    SNAP, V5
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229680-plasmid-map-sequence-id-457075

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  17. pML340_RAB11A-N-term_XTEN80-mEGFP-3xHA_pTMPLT

    Plasmid
    #227907
    Purpose
    Homology repair template for genome engineering. Organelle endogenous marker expressing GFP and epitope tags for mass spectrometry.
    Depositor
    Manuel Leonetti
    Article
    Hein et al Cell. 2024 Dec 26:S0092-8674(24)01344-8.
    Insert
    RAB11A homology arms with XTEN80-mEGFP-3xHA for insertion at N terminus
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    227907-plasmid-map-sequence-id-452503

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Showing: 1841 - 1860 of 1954 results