We narrowed to 7,819 results for: Lif

1. Tol2pA2-drl:APEX2-mCherry

   Plasmid

   #188945

   Purpose

   Plasmid for Tol2 transgenesis expressing APEX2 tagged to mitochondria and nuclei, and cytoplasmic mCherry, driven by the draculin promoter

   Depositor

   Owen Tamplin

   Article

   Agarwala et al Elife. 2022 Aug 9;11. pii: 64835. doi: 1

   Insert

   drl:mito-APEX2_p2A_APEX2-H2B_p2A_mCherry_polyA
   

   Use

   Tol2 destination vector (attr4-r3)

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. CVS-N2c-nl.EGFP-FlpO

   Plasmid

   #172379

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   nuclear-localized EGFP + FlpO
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CSII-IDR2-owlTRIMCyp-FOS

   Plasmid

   #79067

   Purpose

   Express owl monkey TRIMCyp in mammalan cells

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   TRIM5Cyp (owl monkey)-FOS
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLX208-gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4

   Plasmid

   #125230

   Purpose

   For generating SPARK NanoLuc-oβ2AR transmembrane component lentivirus

   Depositor

   Alice Ting

   Article

   Kim et al eLife. 2019 Apr 3;8. pii: 43826. doi: 10

   Insert

   gLuc sp-NanoLuc-HA-oβ2AR-TS-eLOV-TEVcs-Flag-Gal4
   

   Use

   Lentiviral

   Tags

   HA,Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-Nxph4-HA

   Plasmid

   #128102

   Purpose

   Express HA tagged Nxph4

   Depositor

   Huda Zoghbi

   Article

   Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

   Insert

   Nxph4 (Nxph4 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. CVS-N2c-SypRFP

   Plasmid

   #172381

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   Synaptophysin1-RFP fusion protein
   

   Use

   Neurotropic virus

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPW3783 : CMVd3-ERmembrane-GST-2xHaloTag-KKMP

   Plasmid

   #185687

   Purpose

   Low-level transient expression of two tandem HaloTag proteins targeted to the ER membrane, fused with a GST for constitutive dimerization (making a HaloTag tetramer).

   Depositor

   Peter Walter

   Article

   Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

   Insert

   ERmembrane-GST-2xHaloTag-KKMP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. BoxB-MS2 adRNA (RAB7A-ACG site)

   Plasmid

   #170155

   Purpose

   AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editing

   Depositor

   Prashant Mali

   Article

   Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

   Insert

   BoxB-RAB7A(ACG)-MS2
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-CAG-EGFPnls barcode-206-SV40 polyA

   Plasmid

   #190875

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFPnls-barcode206
   

   Use

   AAV

   Tags

   Expression

   Mutation

   WT

   Promoter

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2

    Plasmid

    #127360

    Purpose

    gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NLS, V5, and YFP

    Expression

    Mutation

    R118G

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDONR221 Cas9-RFC5

    Plasmid

    #183200

    Purpose

    Entry clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    Cas9-RFC5
    

    Use

    CRISPR; Gateway entry clone

    Tags

    Expression

    Mutation

    n/a

    Promoter

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pUC-STITCH

    Plasmid

    #129535

    Purpose

    STITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.

    Depositor

    Hideyuki Okano

    Article

    Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10

    Insert

    STITCH
    

    Use

    CRISPR; Donor template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MS2-adRNA (5'UAG)

    Plasmid

    #170126

    Purpose

    Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    MS2-ADAR2(5'UAG)-MS2
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 and mouse U6

    Available since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. BoxB-MS2 adRNA (RAB7A-UAG site)

    Plasmid

    #170138

    Purpose

    AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

    Depositor

    Prashant Mali

    Article

    Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

    Insert

    BoxB-RAB7A(UAG)-MS2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-CAG-EGFP barcode-10-SV40 polyA

    Plasmid

    #190874

    Purpose

    For barcoded retrograde labeling

    Depositor

    Yuejun Chen

    Article

    Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

    Insert

    EGFP-barcode10
    

    Use

    AAV

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. CVS-N2c-EGFP-FlpO

    Plasmid

    #172373

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    EGFP + FlpO
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CVS-N2c-jGCaMP8f

    Plasmid

    #172387

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    GCaMP8f
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2

    Plasmid

    #127357

    Purpose

    gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice

    Depositor

    Dominique Bergmann

    Article

    Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

    Insert

    BirA* (R118G mutant)
    

    Use

    Gateway entry vector

    Tags

    GS linker, NES, V5, and YFP

    Expression

    Mutation

    R118G

    Promoter

    no promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40

    Plasmid

    #159672

    Purpose

    Plasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.

    Depositor

    Omar Akbari

    Article

    Kandul et al Elife. 2021 Mar 5;10. pii: 65939. doi: 1

    Insert

    βTub-NLS-hSpCas9-T2A-GFP
    

    Use

    Tags

    T2A-GFP

    Expression

    Insect

    Mutation

    Promoter

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. CVS-N2c-nl.EGFP-tdTomato

    Plasmid

    #172382

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Yoav Ben-Simon, Peter Jonas

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    nuclear-localized EGFP + tdTomato
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge