Addgene

We narrowed to 13,707 results for: TIM

Showing: 1841 - 1860 of 13707 results

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pcDNA5/FRT/TO_HaloTag7_SKL

Plasmid

#175532

Purpose

Peroxysomal expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag7-SKL

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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GPI_2xmCherry

Plasmid

#127812

Purpose

Homo-dimer mCherry-mCherry expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

glycosylphosphatidylinositol-mCherry-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCIS.MTRIA-DA

Plasmid

#184603

Purpose

For expression of a fluorescent sensor for dopamine in mammalian cells

Depositor

Hiroshi Hibino, Daisuke Ino

Article

Ino et al Nat Methods. 2022 Sep 22. pii: 10.1038/s

Insert

MTRIA-DA

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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beta2 (px330:2-guideCas9)

Plasmid

#169435

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCVII-BFP

Plasmid

#162755

Purpose

Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

Depositor

Maki Nakayama

Article

Landry et al Bio Protoc. 2021 Jan 20;11(2):e3883. doi

Insert

mTagBFP2

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJKR-L-cdaR

Plasmid

#62558

Purpose

Glucarate biosensor. Low copy plasmid that expresses GFP proportional to glucarate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pmTurquoise-N1

Plasmid

#60559

Purpose

Expresses the cyan fluorescent protein variant mTurquoise

Depositor

Dorus Gadella

Article

Goedhart et al Nat Methods. 2010 Feb;7(2):137-9. doi: 1

Insert

mTurquoise

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pZE-PpadC-yveF-yveG-egfp

Plasmid

#201791

Purpose

the promoter can be recognized by PadR

Depositor

Yajun Yan

Article

Jiang et al ACS Synth Biol. 2021 Jan 15;10(1):132-14

Insert

PpadC-yveF-yveG

Use

Tags

Expression

Bacterial

Mutation

WT

Promoter

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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GluA3 (px330:2-guideCas9)

Plasmid

#169424

Purpose

Expression of Cas9 and 2 guides

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Insert

spCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pJKR-L-mphR

Plasmid

#62560

Purpose

Macrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pJKR-L-acuR

Plasmid

#62568

Purpose

Acrylate biosensor. Low copy plasmid that expresses GFP proportional to acrylate concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HisFlag-CAD

Plasmid

#31615

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al Nucleic Acids Res. 2004 . 32(15):4524-3

Insert

CAD

Use

Tags

3xFlag, His, and T7

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
DL147_plenti_TRE_HT-iPAK4

Plasmid

#187447

Purpose

Tet-On inducible expression of HaloTag-iPAK4 in a lentiviral backbone

Depositor

Adam Cohen

Article

Lin et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

INKA-box Pak4

Use

Lentiviral

Tags

halo tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pEB1-mTurquoise2

Plasmid

#103974

Purpose

Plasmid encoding mTurquoise2

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mTurquoise2

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pEB1-mTurquoise

Plasmid

#103972

Purpose

Plasmid encoding mTurquoise

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mTurquoise

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, Y66W, …

Promoter

proC

Available since

Dec. 15, 2017

Availability

Academic Institutions and Nonprofits only

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CS2 Flag-Smad3 (3A)

Plasmid

#14964

Depositor

Joan Massague

Article

Kretzschmar et al Genes Dev. 1999 Apr 1. 13(7):804-16.

Insert

Smad3 (3A) (SMAD3 Human)

Use

Xenopus/mammalian/avian/zebrafish

Tags

Flag

Expression

Mutation

Three Ser to Ala mutations in the phosphorylation…

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pEB1-mEmerald

Plasmid

#103982

Purpose

Plasmid encoding mEmerald

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mEmerald

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F64L, S65T, …

Promoter

proC

Available since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomSua1

Plasmid

#107446

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Sua1

Use

Tags

V5-His

Expression

Yeast

Mutation

See depositor comments below.

Promoter

GAL1

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pYES2.1-pomMet14

Plasmid

#107451

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met14 (met14 Fission Yeast)

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pmTurquoise-C1

Plasmid

#60558

Purpose

Expresses the cyan fluorescent protein variant mTurquoise

Depositor

Dorus Gadella

Article

Goedhart et al Nat Methods. 2010 Feb;7(2):137-9. doi: 1

Insert

mTurquoise

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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