We narrowed to 16,166 results for: grna

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pT7-gRNA:Asip

Plasmid

#60968

Purpose

Expression vector of rat Asip guide RNA

Depositor

Tomoji Mashimo

Publication

Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

Insert

Agouti signaling protein gRNA (Asip Rat)

Use

CRISPR

Available since

Jan. 5, 2015

Availability

Academic Institutions and Nonprofits only
PEA1-Puro

Plasmid

#171991

Purpose

Delivers all prime editing nickase components in a single, puromycin selectable plasmid

Depositor

Paul Thomas

Publication

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Expression

Mammalian

Promoter

CMV for Cas9, U6 for gRNAs

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p-AAV-sh[control]

Plasmid

#75438

Purpose

control shRNA vector

Depositor

Edward Burton

Publication

Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

Insert

GFP

Use

AAV and RNAi

Expression

Mammalian

Promoter

U6

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pRetrosuper Fbw7 shRNA-2

Plasmid

#15661

Depositor

Martin Eilers

Publication

Popov et al Nat Cell Biol. 2007 Jul . 9(7):765-74.

Insert

Fbw7 shRNA (FBXW7 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Nov. 2, 2007

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
pCRII-Blunt-U6-HES7-STOP-gRNA

Plasmid

#130933

Purpose

gRNA for tag human HES7

Depositor

James Thomson

Publication

Chu et al Cell Rep. 2019 Aug 27;28(9):2247-2255.e5

Insert

HES7-STOP-gRNA (HES7 Human)

Expression

Mammalian

Promoter

U6

Available since

Oct. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO-RFP-shCntrl

Plasmid

#69040

Purpose

Lentiviral expression of control shRNA

Depositor

William Kaelin

Publication

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

control shRNA

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available since

Oct. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLKO human ULK1 shRNA 8

Plasmid

#27633

Depositor

Reuben Shaw

Publication

Egan et al Science. 2011 Jan 28;331(6016):456-61. d

Insert

human ULK1 shRNA 8

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pRetroSuper-GFP Smad2

Plasmid

#15722

Depositor

Joan Massague

Publication

He et al Cell. 2006 Jun 2. 125(5):929-41.

Insert

Smad2 shRNA (SMAD2 Human)

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Sept. 7, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pSUPER.ATMi

Plasmid

#14581

Depositor

Didier Trono

Publication

Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

Insert

ATM shRNA (ATM Human)

Use

RNAi

Expression

Mammalian

Available since

March 27, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
px458_2A_GFP_sgRNA_ELAVL1

Plasmid

#106106

Purpose

CRISPR knockout. Expresses Cas9, EGFP, and sgRNA targeting ELAVL1

Depositor

Thomas Tuschl

Publication

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

gRNA targeting ELAVL1 (ELAVL1 Human)

Use

CRISPR

Available since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pGH020_sgRNA_G418-GFP

Plasmid

#85405

Purpose

hu6 driven sgRNA vector with G418 and GFP selectable markers

Depositor

Michael Bassik

Publication

Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

G418 resistance and GFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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UbC-TRE-mSOD2-tTS-IRES-EGFP

Plasmid

#12390

Depositor

Zuoshang Xu

Publication

Xia et al Biotechniques. 2006 Jul . 41(1):64-8.

Insert

mSOD2 shRNA (Sod2 Mouse)

Use

RNAi

Expression

Mammalian

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLVUHshp53-tTR-KRAB

Plasmid

#11654

Purpose

Tet-regulated (Tet-on) lentiviral vector for shp53 (hUbiquitin promoter) - 3rd generation

Depositor

Patrick Aebischer, Didier Trono

Publication

Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

Insert

hUbiquitin C, GFP, tTR-KRAB, shRNA against TP53, no tetO (TP53 Human)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Available since

Aug. 17, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TetON-shYAP

Plasmid

#115667

Purpose

Inducible YAP knockdown

Depositor

Joan Massague

Publication

Er et al Nat Cell Biol. 2018 Aug;20(8):966-978. d

Insert

YAP shRNA (YAP1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Oct. 9, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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MSCV-Luciferase-IRES-NrasG12D

Plasmid

#60834

Purpose

Mouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRES

Depositor

Scott Lowe

Publication

Zuber et al Genes Dev. 2009 Apr 1;23(7):877-89. doi:

Insert

NrasG12D (Nras Mouse)

Use

Retroviral

Available since

Nov. 13, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pSMP-Luc

Plasmid

#36394

Depositor

George Daley

Publication

Onder et al Nature. 2012 Mar 4;483(7391):598-602. do

Insert

Firefly Luciferase

Use

RNAi and Retroviral

Expression

Mammalian

Available since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles
pRP[CRISPR]-EGFP/Puro-hCas9-U6>(long left)-U6>(long right)

Plasmid

#216871

Purpose

This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

Depositor

Ori Bar-Nur

Publication

Lenardic et al J Clin Invest. 2024 May 7:e166998. doi:

Insert

Cas9, gRNA Long.L, gRNA Long.R (Dmd Mouse)

Use

CRISPR

Mutation

mdx mutation in mouse dystrophin

Available since

June 3, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90)

Plasmid

#226989

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1a must be used with gRNA 2a or 2b

Depositor

Benjamin Kleinstiver

Publication

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA 1a to create OPA1-del_exon5

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91)

Plasmid

#226991

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1b

Depositor

Benjamin Kleinstiver

Publication

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA 2a to create OPA1-del_exon5

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

Plasmid

#226992

Purpose

pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b

Depositor

Benjamin Kleinstiver

Publication

Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

Insert

SpCas9 gRNA 2b to create OPA1-del_exon5

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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