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We narrowed to 3,293 results for: actin

Showing: 1861 - 1880 of 3293 results

  5. pCAG CAPN7 V18D,F98D-OSF

    Plasmid
    #193045
    Purpose
    Mammalian expression plasmid encoding full-length CAPN7 with V18D and F98D mutations that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    CAPN7 (CAPN7 Human)
    Tags
    OSF (One-Strep-FLAG)
    Expression
    Mammalian
    Mutation
    V18D, F98D
    Promoter
    Chicken Beta-actin
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193045-plasmid-map-sequence-id-378994

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  9. TLNRD1(4H)-2E_pET151

    Plasmid
    #224068
    Purpose
    Bacterial expression of the 4-helix bundle of TLNRD1 (residues 143-273) containing the mutations K192E/R233E, fused to an N-terminal hexa-histidine tag. TEV cleavage site
    Depositor
    Ben Goult
    Article
    Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003
    Insert
    tlnrd1 (TLNRD1 Human)
    Tags
    6xHIS; V5
    Expression
    Bacterial
    Mutation
    K192E/R233E double mutant; prevents actin binding…
    Promoter
    T7
    Available Since
    Aug. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    224068-plasmid-map-sequence-id-443085

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  10. qTAG-N-Solo-mScarlet-ACTB

    Plasmid
    #207750
    Purpose
    Donor template for mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a mScarlet Tag (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207750-plasmid-map-sequence-id-414814

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  11. qTAG-N-Solo-mNeon-ACTB

    Plasmid
    #207749
    Purpose
    Donor template for mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a mNeon Tag (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    207749-plasmid-map-sequence-id-414795

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  14. pCAGGS-SMS1-TEV-HAT

    Plasmid
    #202557
    Purpose
    Express C-terminal Tobacco Etch Virus (TEV) protease cleavable HAT-tagged human Sphingomyelin synthase 1 (SMS1) in mammalian cell
    Depositor
    Chiaki Murakami
    Article
    Furuta et al FEBS Open Bio. 2023 May 11. doi: 10.1002
    Insert
    SGMS1 (SGMS1 Human)
    Tags
    Histidine-Affinity-tag (HAT) and TEV cleavable si…
    Expression
    Mammalian
    Mutation
    deleted stop codon (TAA)
    Promoter
    CAG and chicken β-actin promoter
    Available Since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    202557-plasmid-map-sequence-id-399621

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  15. pORANGE_Tubb3 GFP(Y39TAG) KI

    Plasmid
    #182678
    Purpose
    Expression of spCas9, gRNA targeting the end of Tubb3 gene and donor GFP(Y39TAG). Can be used for amber codon suppression and click chemistry labeling of endogenous beta-3 tubulin in mammalian cells.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    Tubb3-targeting gRNA and GFP(Y39TAG) donor sequence (Tubb3, Tubb3 Mouse, Rat)
    Expression
    Mammalian
    Promoter
    U6 and chicken beta-actin promoter
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182678-plasmid-map-sequence-id-363084

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  16. TKIT NFL gRNA1 gRNA2

    Plasmid
    #182681
    Purpose
    Expression of spCas9, gRNA targeting intron 3 and gRNA targeting 3'UTR of the mouse Nefl gene. Can be used for the tagging of endogenous NFL via Targeted Knock-In with Two guides (TKIT) approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    two Nefl-targeting gRNAs, one targets intron 3 and the other 3'UTR of the Nefl gene (Nefl Mouse)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    U6 and chicken beta-actin promoter
    Available Since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182681-plasmid-map-sequence-id-363020

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  17. pTone-gata2aECE-nsGFP

    Plasmid
    #132975
    Purpose
    gata2a endothelial enhancer (x6) and basal promoter driving nuclear-localized sfGFP in pTol1 backbone
    Depositor
    Nathan Lawson
    Article
    Shin et al Dev Cell. 2019 Nov 4;51(3):374-386.e5. d
    Inserts
    nuclear localized sfGFP
    gata2a endothelial enhancer (x6) with a carp b-actin basal promoter
    Use
    Unspecified; Transposon-mediated
    Tags
    6x myc and SV40 NLS
    Available Since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    132975-plasmid-map-sequence-id-264810

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  19. pABD1031

    Plasmid
    #68004
    Purpose
    Protein co-localization of PIF1 (PIL5) via fluorescent fusion after transient expression in planta
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 )
    Tags
    eYFP
    Expression
    Plant
    Promoter
    2X Cauliflower Mosaic Virus 35S promoter
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. pABD1275

    Plasmid
    #68017
    Purpose
    PIF1 protein overproduction for purification from bacterial cells
    Depositor
    Bruce Downie
    Article
    Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11
    Insert
    PHY-INTERACTING FACTOR 1 (PIF1) (PIL5 AT2G20180, Mustard Weed)
    Tags
    6x-His
    Expression
    Bacterial
    Mutation
    Codon Optimized for bacterial expression (GenScri…
    Promoter
    T7
    Available Since
    Dec. 17, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 1861 - 1880 of 3293 results