We narrowed to 6,524 results for: cki

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pSEED-Dendra2

Plasmid

#219645

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Dendra2 using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

Dendra2 SEED Cassette

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMRX-IP TFEB-sfGFP

Plasmid

#135402

Purpose

Expresses TFEB-sfGFP

Depositor

Eisuke Itakura

Article

Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

Insert

TFEB (TFEB Human)

Use

Retroviral

Tags

sfGFP

Expression

Mammalian

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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APEX2-csGBP

Plasmid

#108874

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against GFP

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-csGBP

Expression

Mammalian

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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APEX2-csChBP

Plasmid

#108876

Purpose

Expression of APEX2 fused to a conditionally stable nanobody against mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

APEX2-csChBP

Expression

Mammalian

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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V5-GFP-TurboID-KDEL_pLX208

Plasmid

#202020

Purpose

expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-TurboID-KDEL

Use

Lentiviral

Tags

GFP and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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WT WWP1

Plasmid

#200085

Purpose

WWP1 in pQCXIN vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

WT WWP1 (WWP1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pSEED-sfGFP

Plasmid

#219639

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP SEED Cassette

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED

Plasmid

#219658

Purpose

Permits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

Empty SEED Cassette (3xP3-dsRed)

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Endosomes-14

Plasmid

#58014

Purpose

Localization: Endosomes, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Endosomes (RHOB Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Industry, Academic Institutions, and Nonprofits
nls-mCherry

Plasmid

#108881

Purpose

Expression of nuclear localized mCherry

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

nls-mCherry

Use

Can be used for mammalian expression or in vitro …

Expression

Mammalian

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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413-Met25 Kar2 TorsinA E171Q GFP

Plasmid

#32127

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

GFP and Kar2

Expression

Yeast

Mutation

E171Q

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only
413-Met25 Kar2 TorsinA K108A GFP

Plasmid

#32125

Depositor

Susan Lindquist

Article

Valastyan et al PLoS One. 2011;6(7):e22744. Epub 2011 Ju

Insert

TorsinA (TOR1A Human)

Tags

GFP, HDEL, and Kar2

Expression

Yeast

Mutation

K108A

Available Since

Nov. 14, 2011

Availability

Academic Institutions and Nonprofits only
ST-GFP

Plasmid

#162500

Purpose

Expresses ST6GAL1 with a GFP tag in its C-terminus in mammalian cells

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

ST6Gal1 (ST6GAL1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pN-PITCh-mCherry

Plasmid

#127887

Purpose

Provides the repair template with PP2Ac microhomology on each end for PP2Ac N-ter mCherry tagging

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Insert

Puro-mCherry

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-Sec31a 4SA #33#638

Plasmid

#42124

Depositor

Ayano Satoh

Article

Koreishi et al PLoS One. 2013;8(1):e54382. doi: 10.1371

Insert

Sec31a (SEC31A Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

S527A S799A S1163A T1165A

Promoter

CMV

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pFETCh_Donor (EMM0021)

Plasmid

#63934

Purpose

3X Flag with P2A-Neo for 3' tagging of endogenous proteins

Depositor

Eric Mendenhall, Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

Glycine-Serine-3XFlag-P2A-NeoR

Expression

Mammalian

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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pRRL sEF1a HA.NLS.Sce(opt).T2A.IFP

Plasmid

#31484

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

EF1s HA NLS Sce(opt) T2A IFP1.4

Use

Lentiviral

Tags

HA and NLS

Mutation

"Opt" indicates the I-SceI coding seque…

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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GLUT1-GFP

Plasmid

#89440

Purpose

GLUT1 construct

Depositor

Jeffrey Rathmell

Article

Wieman et al Biochem J. 2009 Mar 1;418(2):345-67. doi

Insert

GLUT1 (Slc2a1 Rat)

Tags

GFP

Expression

Mammalian

Promoter

EF-1a

Available Since

May 9, 2017

Availability

Academic Institutions and Nonprofits only

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H2B-mCherry

Plasmid

#108880

Purpose

Expression of human histone2B fused to mCherry (nuclear marker)

Depositor

Rob Parton

Article

Ariotti et al PLoS Biol. 2018 Apr 5;16(4):e2005473. do

Insert

H2B-mCherry

Use

Can be used for mammalian expression or in vitro …

Expression

Mammalian

Available Since

June 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-BI

Plasmid

#126475

Purpose

High-copy number vector for the co-expression of two genes in mammalian cells

Depositor

Bill Hansson

Article

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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