We narrowed to 2,325 results for: tub

1. pcDNA5-FRT-TO-vsv-Incenp 8KQ-GFP

   Plasmid

   #108506

   Purpose

   inducible expression of vsv-INCENP 8KQ-EGFP

   Depositor

   Susanne Lens

   Article

   van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

   Insert

   INCENP (INCENP Human)
   

   Use

   Tags

   EGFP and VSV

   Expression

   Mammalian

   Mutation

   K548Q, K563Q, K602Q, K609Q, K614Q, K622Q, K630Q, …

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1 E2F1 Flag(Δ193-359) (HindIII- BamHI- EcoRI)

   Plasmid

   #70667

   Purpose

   Human mutant of E2F1 lacking amino acids 193-359

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   E2F1 lacking residues 193 to 359 (E2F1 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   lacks amino acids 193-359

   Promoter

   cmv

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1 E2F1 Flag(Δ 284−359)(HindIII- BamHI- EcoRI)

   Plasmid

   #70669

   Purpose

   Human mutant of E2F1 lacking amino acids 284 to 359

   Depositor

   Maria Alvarado-Kristensson

   Article

   Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

   Insert

   E2F1 lacking residues 284 to 359 (E2F1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   lacks amin acids 284 to 359

   Promoter

   cmv

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pET21a-MKP7_K62R_153

   Plasmid

   #37795

   Depositor

   SeWon Suh

   Article

   Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

   Insert

   Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Lysnie 62 to Arginine and deleted amino a…

   Promoter

   Available since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pET21a-MKP7_K52R_153

   Plasmid

   #37793

   Depositor

   SeWon Suh

   Article

   Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

   Insert

   Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   changed Lysnie 52 to Arginine and deleted amino a…

   Promoter

   Available since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pET28a_DmKHC[1-421]-1xGFP-6xHis

   Plasmid

   #196972

   Purpose

   Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal GFP tag and C-terminal 6xHis tag

   Depositor

   Lukas Kapitein

   Article

   Tas et al Neuron. 2017 Dec 20;96(6):1264-1271.e5.

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Use

   Tags

   6xHis and GFP

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR-XL-rAkap9

   Plasmid

   #196867

   Purpose

   Cloned rat (r) A-Kinase Anchoring Protein 9 (Akap9) ORF. Used as a template for Akap9 containing constructs

   Depositor

   Frank Bradke

   Article

   Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

   Insert

   Akap9 (Akap9 Rat)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac promoter and T7 promoter

   Available since

   May 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pOmnibac_ZZ_linker_K963_GFP_SNAP

   Plasmid

   #159727

   Purpose

   Expresses kinesin-1 (amino acids 1-963) in Sf9 cells with a C-terminal GFP and SNAP tag

   Depositor

   Ahmet Yildiz

   Article

   Ferro et al Science. 2022 Jan 21;375(6578):326-331.

   Insert

   KIF5B (full length, 1-963 amino acids), K963 (KIF5B Human)
   

   Use

   Tags

   GFP and SNAP tags and ZZ affinity tag

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBrain-ch-TOGKDP-GFP-shch-TOG

   Plasmid

   #69113

   Purpose

   Dual-promoter plasmid to express knockdown-proof human ch-TOG tagged with EGFP along with shRNA to knock down endogenous ch-TOG in mammalian cells.

   Depositor

   Stephen Royle

   Article

   Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

   Inserts

   Colonic Hepatic Tumour Over-expressed Gene (CKAP5 Human)
   shch-TOG
   

   Use

   RNAi

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Silent mutations to confer shRNA resistance.

   Promoter

   CMV

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid

    #99277

    Purpose

    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance

    Depositor

    Jordan Miller

    Article

    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

    Inserts

    H2B-mOrange2-IRES-EGFP
    Puro
    

    Use

    Lentiviral

    Tags

    EGFP, H2B, IRES, PGK, and mOrange2

    Expression

    Mutation

    Promoter

    EF1 alpha-human and PGK-mouse

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

    Plasmid

    #99278

    Purpose

    Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance

    Depositor

    Jordan Miller

    Article

    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

    Inserts

    H2B-mVenus-IRES-mCherry
    Puro
    

    Use

    Lentiviral

    Tags

    H2B, IRES, PGK, mCherry, and mVenus

    Expression

    Mutation

    Promoter

    EF1 alpha-human and PGK-mouse

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBactin-Nedd1-mOrange2-hCM1

    Plasmid

    #196863

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-hCM1 (Nedd1 Rat, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-lox-inv[Talpha1-iCre-pA]-lox-mNeonGreen-hCentrin1

    Plasmid

    #196890

    Purpose

    Neuron-specific expression of human (h)Centrin fused to mNeonGreen

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mNeonGreen-hCentrin1 (CETN1 Human)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Quimeric CAG

    Available since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOmnibac_ZZ_linker_K560_GFP_SNAP

    Plasmid

    #159720

    Purpose

    Expresses kinesin-1 (amino acids 1-560) in Sf9 cells with a C-terminal GFP and SNAP tag

    Depositor

    Ahmet Yildiz

    Article

    Ferro et al Science. 2022 Jan 21;375(6578):326-331.

    Insert

    KIF5B (only amino acids 1-560), K560 (KIF5B Human)
    

    Use

    Tags

    GFP and SNAP tags and ZZ affinity tag

    Expression

    Insect

    Mutation

    Only amino acids 1-560

    Promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBactin-td-mCherry-Cdk5rap2

    Plasmid

    #196859

    Purpose

    Expression of Cyclin dependent kinase 5 regulatory subunit-associated protein 2 (Cdk5rap2) fused to tandem (td)-mCherry.

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    td-mCherry-Cdk5rap2 (Cdk5rap2 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. VN-PTEN

    Plasmid

    #89331

    Purpose

    expresses hPTEN fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Marciniak et al J Exp Med. 2017 Aug 7;214(8):2257-2269.

    Insert

    PTEN1 (PTEN Human)
    

    Use

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBrain-GFP-TACC3KDP∆682-688-shTACC3

    Plasmid

    #69116

    Purpose

    Dual-promoter plasmid to express knockdown-proof human TACC3 (682-688aa ch-TOG binding site deleted) tagged with EGFP along with shRNA to knock down endogenous TACC3 in mammalian cells.

    Depositor

    Stephen Royle

    Article

    Gutierrez-Caballero et al Biol Open. 2015 Jan 16;4(2):170-9. doi:

    Inserts

    Transforming Acidic Coiled Coil protein 3 (TACC3 Human)
    shTACC3
    

    Use

    RNAi

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 682-688 (region containin…

    Promoter

    CMV

    Available since

    Oct. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP(5X)

    Plasmid

    #218426

    Purpose

    Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A

    Depositor

    Dieter Willbold

    Article

    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

    Insert

    GABARAP(G116A/5X)-mTagBFP2-GABARAP(5X) (GABARAP Entacmaea quadricolor, Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    5X: K2A, K13A, R15A, K20A, K23A mutations of both…

    Promoter

    CMV

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA5/FRT/TO GABARAP(G116A)-mTagBFP2-GABARAP(5X)

    Plasmid

    #218425

    Purpose

    Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A

    Depositor

    Dieter Willbold

    Article

    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

    Insert

    GABARAP(G116A)-mTagBFP2-GABARAP(5X) (GABARAP Entacmaea quadricolor, Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    5X: K2A, K13A, R15A, K20A, K23A mutations of down…

    Promoter

    CMV

    Available since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5/FRT/TO GABARAP(5X/G116A)-mTagBFP2-GABARAP

    Plasmid

    #212107

    Purpose

    Expression of GABARAP split tandem protein with mTagBFP2, 5X: K2A, K13A, R15A, K20A, K23A

    Depositor

    Dieter Willbold

    Article

    Uffing et al Autophagy Rep. 2024 May 16;3(1):2348899.

    Insert

    GABARAP(5X/G116A)-mTagBFP2-GABARAP (GABARAP Entacmaea quadricolor, Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    5X: K2A, K13A, R15A, K20A, K23A mutations of upst…

    Promoter

    CMV

    Available since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge