We narrowed to 120,450 results for: CIL

1. pHiFi Cas9-2×sgRNA (empty, donor)

   Plasmid

   #162277

   Purpose

   All-in-one vector for CRISPR/Cas9-mediated homology-independent knock-in system. The plasmid contains BPNLS-HiFi Cas9-BPNLS and two sgRNA expression cassettes.

   Depositor

   Kazuhisa Nakayama

   Article

   Fujisawa et al Biol Open. 2021 Aug 27. pii: 271969. doi

   Insert

   HiFi Cas9
   

   Use

   CRISPR

   Tags

   BPNLS and FLAG tag

   Expression

   Mammalian

   Mutation

   SpCas9 (R691A)

   Available Since

   Aug. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. FUW TetO HA-AMPKa1 WT

   Plasmid

   #69824

   Purpose

   Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Promoter

   minimal CMV promoter with tetracycline operator

   Available Since

   April 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5FRT/TO-Npl4-Strep-HA

   Plasmid

   #113495

   Purpose

   Expression of human Npl4 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   Npl4 (NPLOC4 Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5FRT/TO-Ufd1-Strep-HA

   Plasmid

   #113474

   Purpose

   Expression of human Ufd1 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   Ufd1 (UFD1 Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

   Plasmid

   #133794

   Purpose

   U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018

   Depositor

   Joseph Bondy-Denomy, Benjamin Kleinstiver

   Article

   Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

   Insert

   NmeCas9 and Nme2Cas9 sgRNA entry vector
   

   Expression

   Mammalian

   Mutation

   sgRNA architecture from Amrani et al. Genome Biol…

   Promoter

   U6

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET-EIN-AlkBH5(66-292)

   Plasmid

   #125732

   Purpose

   Plasmid with EIN as a solubility and expression enhancing tag to increase production of hard to express proteins such as AlkBH5 and TEV cleaving site for the removal of the fusion tag.

   Depositor

   Vincenzo Venditti

   Article

   Khatiwada et al Protein Expr Purif. 2019 Nov 15;167:1055

   Insert

   AlkBH5(66-292) (ALKBH5 Human)
   

   Tags

   6xHis and EIN

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pB-Gaolf-Integration

   Plasmid

   #129456

   Purpose

   piggyBac transposon vector that Inducibly (Tet-On) expresses Gaolf

   Depositor

   Sriram Kosuri

   Article

   Jones et al Cell Syst. 2019 Mar 27;8(3):254-260.e6.

   Insert

   G alpha olf (Gnal Mouse)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   TRE (Tet-On)

   Available Since

   Aug. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. HA-Rab8a-CA (Q67L)

   Plasmid

   #101050

   Purpose

   Expresses HA-tagged human Rab8-CA (Q67L) in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Article

   Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

   Insert

   Rab8a (RAB8A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Q67L

   Promoter

   CMV

   Available Since

   Sept. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCS2- mRuby-Rab35

   Plasmid

   #194338

   Purpose

   Expresses mRuby Rab35 in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Aljiboury et al PLoS Genet. 2023 May 15;19(5):e1010765.

   Insert

   Rab35 (RAB35 Human)
   

   Tags

   mRuby

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Jan. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. TALED_Right-mouse-ATP6-1397C-AD(V28R)

    Plasmid

    #212955

    Purpose

    Expresses TALED_Right-mouse-ATP6-1397C-AD(V28R) in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

    Insert

    Right-mouse-ATP6-1397C-AD(V28R) (ATP6 Synthetic)
    

    Expression

    Mammalian

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCSIIpuro-mNG-eDHFR(69K6)-RasGEF

    Plasmid

    #172104

    Purpose

    Mammalian expression of RasGRF1(Cdc25) fused to mNeonGreen-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    mNeonGreen-eDHFR(69K6)-RasGRF1(Cdc25) (RASGRF1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSH-EFIRES-P-GFP(1-10)opti

    Plasmid

    #129416

    Purpose

    Expressing codon-optimized GFP(1-10) fragment in human cells

    Depositor

    Elina Ikonen

    Article

    Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

    Insert

    codon-optimized GFP(1-10)
    

    Use

    CRISPR, TALEN, and Unspecified ; Human safe harbo…

    Expression

    Mammalian

    Promoter

    EF-1α

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. HA-Rab8a-DN (T22N)

    Plasmid

    #101049

    Purpose

    Expresses HA-tagged human Rab8-DN (T22N) in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Article

    Burke et al J Cell Biol. 2014 Oct 13;207(1):123-37.

    Insert

    Rab8a (RAB8A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    T22N

    Promoter

    CMV

    Available Since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A

    Plasmid

    #212949

    Purpose

    Expresses TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A in mammalian cells

    Depositor

    Jin-Soo Kim

    Article

    Cho et al Cell. 2024 Jan 4;187(1):95-109.e26. doi:

    Insert

    TALED_ Left-mouse-mtRnr1_A1555G-8e-E1347A (mt-Rnr1 Synthetic)
    

    Expression

    Mammalian

    Available Since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCDNA5-TEX264-deltaLRR-cSSH

    Plasmid

    #220210

    Purpose

    Mammalian expression of TEX264, 2-25 residues (LRR) truncated, C-terminal twin-Strep_HA tag

    Depositor

    Kristijan Ramadan

    Article

    Fielden et al Nat Commun. 2020 Mar 9;11(1):1274. doi:

    Insert

    TEX264 (TEX264 Human)
    

    Tags

    Strep-strep-HA

    Expression

    Mammalian

    Mutation

    c.4-75del / p.2-24del

    Promoter

    CMV/TetO

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA5-PKHD1-myc

    Plasmid

    #108407

    Purpose

    Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

    Depositor

    Gregory Germino

    Article

    Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

    Insert

    PKHD1 (PKHD1 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-GFP-PKHD1

    Plasmid

    #108408

    Purpose

    Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

    Depositor

    Gregory Germino

    Article

    Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

    Insert

    PKHD1 (PKHD1 Human)
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. hA3A-BE3 (pRZ215)

    Plasmid

    #131314

    Purpose

    CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3-EGFP-AC8(1-106)

    Plasmid

    #181958

    Purpose

    Residues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.

    Depositor

    Jin Zhang

    Article

    Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

    Insert

    EGFP-AC8(1-106) (Adcy8 Rat)
    

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. hAID-BE3 (pRZ352)

    Plasmid

    #131316

    Purpose

    CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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