Addgene

We narrowed to 17,056 results for: URE

Showing: 1881 - 1900 of 17056 results

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pAAV-mCherry-flex-dtA

Plasmid

#58536

Purpose

Expresses diphtheria toxin A subunit in Cre positive cells, and mCherry in all the infected cells

Depositor

Naoshige Uchida

Article

Wu et al Nature. 2014 May 15;509(7500):325-30. do

Insert

dtA (DIP_RS18250 )

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 22, 2014

Availability

Academic Institutions and Nonprofits only

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SrtA-PDGFR Tm

Plasmid

#121168

Purpose

pMP71 vector expressing Tomato - P2A - flag - SrtA - PDGFR Tm

Depositor

Gabriel Victora

Article

Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

Insert

SrtA-PDGFR

Use

Retroviral

Tags

Tomato and flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-EGFP

Plasmid

#91975

Purpose

EGFP reporter cell line control (no miR-19 sites)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

EGFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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mEGFP-Rac1

Plasmid

#83950

Purpose

Rac1 FLIM donor for dynamic imaging of GTPase activity

Depositor

Ryohei Yasuda

Article

Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

Insert

ras-related C3 botulinum toxin substrate 1 (RAC1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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CD40L-SrtA

Plasmid

#121167

Purpose

pMP71 vector expressing Tomato - P2A - CD40L - SrtA - flag

Depositor

Gabriel Victora

Article

Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

Insert

CD40L-SrtA

Use

Retroviral

Tags

Tomato and flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pJC175e

Plasmid

#79219

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PPSP gIII, luxAB

Use

Tags

Expression

Bacterial

Mutation

Promoter

PPSP

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAS088

Plasmid

#211502

Purpose

AAV genome backbone for AAV-Perturb-seq with direct gRNA capture and nuclei sorting based on eGFP.

Depositor

Randall Platt

Article

Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:

Type

Empty backbone

Use

AAV, CRISPR, Cre/Lox, and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-Flag-mAIM2-EGFP

Plasmid

#51667

Purpose

Express Flag and GFP-tagged mAIM2 in mammalian cells

Depositor

Emad Alnemri

Article

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

Aim2 (Aim2 Mouse)

Use

Retroviral

Tags

EGFP and Flag

Expression

Mammalian

Mutation

Promoter

MESV

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pGCDNsam-Hnf4a

Plasmid

#33002

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

hepatic nuclear factor 4 alpha (Hnf4a Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2012

Availability

Academic Institutions and Nonprofits only

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Flag-Gsdmd-NT

Plasmid

#80951

Purpose

Expresses Gsdmd-NT in mammalian cells

Depositor

Judy Lieberman

Article

Liu et al Nature. 2016 Jul 6;535(7610):153-8. doi:

Insert

Gsdmd-NT (Gsdmd Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-LplA(AILR,36)-NRX3b

Plasmid

#43919

Depositor

Alice Ting

Article

Liu et al PLoS One. 2013;8(2):e52823. doi: 10.1371

Insert

AILR-LplA36-NRX3b (lplA Synthetic, Human)

Use

Tags

2XHA

Expression

Mammalian

Mutation

Promoter

Chicken beta actin

Available since

March 27, 2013

Availability

Academic Institutions and Nonprofits only

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pFW1000

Plasmid

#224210

Purpose

E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), R6K (E. coli replication origin)

Depositor

Ophelia Venturelli

Article

Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-mTUT3

Plasmid

#60040

Purpose

Mammalian expression of FLAG-tagged mouse TUT3

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

TUT3 (Papd5 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
pWUR703

Plasmid

#53082

Purpose

inducible TtAgoDM (D478A,D546A) expression

Depositor

John van der Oost

Article

Swarts et al Nature. 2014 Mar 13;507(7491):258-61. do

Insert

TtAgo

Use

Tags

Strep-II tag

Expression

Bacterial

Mutation

D478A D546A

Promoter

T7

Available since

May 13, 2014

Availability

Academic Institutions and Nonprofits only

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TrkB mEGFP

Plasmid

#83952

Purpose

FRET donor for dynamically measuring TrkB activity in neurons

Depositor

Ryohei Yasuda

Article

Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

Insert

Tropomyosin related kinase B (Ntrk2 Mouse)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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Adeno Cas9

Plasmid

#64072

Purpose

Adenoviral vector for the expression of the Cas9

Depositor

Andrea Ventura

Article

Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

Type

Empty backbone

Use

Adenoviral

Tags

FLAG

Expression

Mutation

Promoter

U6 and CBh

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pFastBac_human-IL-18

Plasmid

#214338

Purpose

Protein overexpression in bacteria

Depositor

Jonathan Kagan

Article

Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

Insert

IL18 (IL18 Human)

Use

Tags

His-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-PAK2 binding domain

Plasmid

#83951

Purpose

FLIM acceptor for mEGFP Rac1

Depositor

Ryohei Yasuda

Article

Hedrick et al Nature. 2016 Sep 28;538(7623):104-108. d

Insert

Pak2 (PAK2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

aa60-113 only; S74A F84A

Promoter

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-Ki67-mCherry

Plasmid

#183748

Purpose

Expresses EGFP-Ki67-mCherry

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (MKI67 Human)

Use

Tags

Ki-67 with N-terminal EGFP tag and Ki-67 with C-…

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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TrkB Acceptor

Plasmid

#83953

Purpose

FRET acceptor for TrkB mEGFP

Depositor

Ryohei Yasuda

Article

Harward et al Nature. 2016 Sep 28;538(7623):99-103. do

Insert

Phospholipase C gamma 1 (Plcg1 Mouse)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

Available since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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