We narrowed to 25,948 results for: Vit;

1. Gi1-CASE

   Plasmid

   #168120

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAI1, GNB1, GNG2 (GNB1, GNG2, GNAI1 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG2

   Expression

   Mammalian

   Mutation

   NLuc is inserted at A121/E122 within GNAI1

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Gi3-CASE

   Plasmid

   #168122

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gi3. Composed of the subunits G alpha i3 (GNAI3) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAI2, GNB1, GNG2 (GNAI1, GNB1, GNG2 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG2

   Expression

   Mammalian

   Mutation

   NLuc is inserted at A114/E115 within GNAI1

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Gq-CASE

   Plasmid

   #168125

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAQ, GNB3, GNG9 (GNAQ, GNB3, GNGT2 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG9

   Expression

   Mammalian

   Mutation

   NLuc is inserted at F124/E125 within GNAQ

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-syn-flex-iGluSnFR4s-PDGFR-WPRE

   Plasmid

   #234439

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s-PDGFR
   

   Use

   AAV and Cre/Lox

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-syn-flex-iGluSnFR4f-PDGFR-WPRE

   Plasmid

   #234440

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-PDGFR
   

   Use

   AAV and Cre/Lox

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCR8 PALI1

   Plasmid

   #114442

   Purpose

   Entry vector for gateway cloning containing human PALI1 coding sequence

   Depositor

   Adrian Bracken

   Article

   Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

   Insert

   PALI1 (LCOR Human)
   

   Use

   Gateway entry cloning

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCR8 LCOR

   Plasmid

   #114441

   Purpose

   Entry vector for gateway cloning containing human LCOR coding sequence

   Depositor

   Adrian Bracken

   Article

   Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

   Insert

   LCOR (LCOR Human)
   

   Use

   Gateway entry cloning

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-U6-AlbEx14-mNG

   Plasmid

   #201780

   Purpose

   knocks in mNeonGreen into exon 14 of Alb to produce Alb-mNeonGreen fusion protein in mice

   Depositor

   Hajime Hirase

   Article

   Vittani et al Fluorescence Imaging of the Brain. Neuro

   Inserts

   Alb gRNA (Alb Mouse)
   Alb_LHA-mNeonGreen-Alb_RHA (Alb Mouse)
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Go1-CASE

   Plasmid

   #168123

   Purpose

   Encodes a BRET-based activity sensor for the heterotrimeric G protein Go1. Composed of the subunits G alpha o1 (GNAO1) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

   Depositor

   Gunnar Schulte

   Article

   Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

   Insert

   GNAO1, GNB3, GNG9 (GNAO1, GNB3, GNGT2 Human)
   

   Use

   Luciferase

   Tags

   cpVenus on GNG9

   Expression

   Mammalian

   Mutation

   NLuc is inserted at L91/G92 within GNAO1

   Promoter

   Available since

   July 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Gs(short)-CASE

    Plasmid

    #168124

    Purpose

    Encodes a BRET-based activity sensor for the heterotrimeric G protein Gs. Composed of the subunits G alpha s(short isoform) (GNAS) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 1 (GNG1).

    Depositor

    Gunnar Schulte

    Article

    Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

    Insert

    GNAS, GNB1, GNG1 (GNGT1, GNAS, GNB1 Human)
    

    Use

    Luciferase

    Tags

    cpVenus on GNG1

    Expression

    Mammalian

    Mutation

    NLuc is inserted at N136/V137 within GNAS

    Promoter

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTRIP-SFFV-Hygro-2A-TMRPSS2

    Plasmid

    #170390

    Purpose

    Generation of stable lines to enhance SARS-CoV-2 infectivity in ACE2 expressing cells

    Depositor

    Nir Hacohen

    Article

    Filbin et al Cell Rep Med. 2021 May 18;2(5):100287. d

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    June 23, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pOLENTE

    Plasmid

    #80683

    Purpose

    DEST vector for cell-free in vitro expression

    Depositor

    Nico Dissmeyer

    Type

    Empty backbone

    Use

    Synthetic Biology; In vitro transcription and tra…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7 and SP6

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pIVTR(T7G)-NS1-TX91

    Plasmid

    #172289

    Purpose

    In vitro transcription of NS1 Influenza A virus protein (strain Texas91) for enhanced in vitro transcribed mRNA translation

    Depositor

    Patrik Verstreken

    Insert

    NS1-TX91
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    T7 promoter G-initiating

    Available since

    Aug. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p28BIOH-LIC

    Plasmid

    #62352

    Purpose

    E. coli expression vector with N-terminal AviTag and a C-terminal Hexa-HIS tag

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and AviTag

    Expression

    Bacterial

    Mutation

    Promoter

    T7-lacO

    Available since

    March 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTW335g3

    Plasmid

    #237325

    Purpose

    expresses S. tuberosum RbcL RbcS-6xHis

    Depositor

    Tina Wang

    Insert

    S. tuberosum RbcL RbcS-6xHis
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTW335f3

    Plasmid

    #237324

    Purpose

    expresses G. max RbcL RbcS-6xHis

    Depositor

    Tina Wang

    Insert

    G. max RbcL RbcS-6xHis
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTW335i3

    Plasmid

    #237323

    Purpose

    expresses N. tabacum RbcL RbcS-6xHis

    Depositor

    Tina Wang

    Insert

    N. tabacum RbcL RbcS-6xHis
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. SDC4A-c001

    Plasmid

    #205119

    Purpose

    SDC4 protein expression

    Depositor

    Paul Brennan

    Insert

    SDC4 (SDC4 Human)
    

    Use

    Tags

    AviTag-6His and PTPRSsp

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTW335h3

    Plasmid

    #237326

    Purpose

    expresses O. sativa RbcL RbcS-6xHis

    Depositor

    Tina Wang

    Inserts

    O. sativa RbcL
    O. sativa RbcS-6xHis
    

    Use

    Tags

    6xHis and None

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTW335p3

    Plasmid

    #237329

    Purpose

    expresses Z. mays RbcL RbcS-6xHis

    Depositor

    Tina Wang

    Inserts

    Z. mays RbcL
    Z. mays RbcS-6xHis
    

    Use

    Tags

    6xHis and None

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTW335m

    Plasmid

    #237328

    Purpose

    expresses M. truncatula RbcL RbcS-6xHis

    Depositor

    Tina Wang

    Inserts

    M. truncatula RbcL
    M. truncatula RbcS-6xHis
    

    Use

    Tags

    6xHis and None

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pJBL6931

    Plasmid

    #217369

    Purpose

    sfGFP reporter for WT Pca rhtB ZTP riboswitch

    Depositor

    Julius Lucks

    Article

    Bushhouse et al bioRxiv 2024.03.29.587317

    Insert

    Pca rhtB riboswitch sfGFP reporter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJBL6951

    Plasmid

    #217373

    Purpose

    sfGFP reporter for Bsu pbuE* 2AP riboswitch

    Depositor

    Julius Lucks

    Article

    Bushhouse et al bioRxiv 2024.03.29.587317

    Insert

    Pca rhtB riboswitch sfGFP reporter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pJBL730

    Plasmid

    #217374

    Purpose

    sfGFP reporter for Bsu yxjA 2AP riboswitch

    Depositor

    Julius Lucks

    Article

    Bushhouse et al bioRxiv 2024.03.29.587317

    Insert

    yxjA riboswitch sfGFP reporter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJBL7368

    Plasmid

    #217375

    Purpose

    sfGFP reporter for Bce crcB fluoride riboswitch

    Depositor

    Julius Lucks

    Article

    Bushhouse et al bioRxiv 2024.03.29.587317

    Insert

    Bce crcB riboswitch sfGFP reporter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pOpen-T5gene122

    Plasmid

    #165544

    Purpose

    Possesses two enzymatic activities: DNA synthesis (polymerase) and exonucleolytic activity that degrades ssDNA in the 3'-5' direction for proofreading purposes.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    T5 DNA Polymerase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 9, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. SLIT2A-c003

    Plasmid

    #205125

    Purpose

    SLIT2 protein expression

    Depositor

    Paul Brennan

    Insert

    SLIT2 (SLIT2 Human)
    

    Use

    Tags

    AviTag-6His and PTPRSsp

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. p28BIOHTEV-LIC

    Plasmid

    #167852

    Purpose

    Bacterial expression vector with N-terminal AviTag and C-terminal 6X-his tag for protein expression and purification

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Use

    Tags

    6xHis and AviTag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 22, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

29. pTW335v3

    Plasmid

    #237330

    Purpose

    expresses H. vulgare RbcL RbcS-6xHis

    Depositor

    Tina Wang

    Inserts

    H. vulgare RbcL
    H. vulgare RbcS-6xHis
    

    Use

    Tags

    6xHis and None

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLenti-hSyn1-mScarlet/CAAX

    Plasmid

    #197592

    Purpose

    Lentiviral transfer vector for the neuronal expression of membrane-bound mScarlet (good visualization of neuronal processes)

    Depositor

    Patrik Verstreken

    Insert

    mScarlet-CAAX
    

    Use

    Lentiviral

    Tags

    CAAX

    Expression

    Mutation

    Promoter

    hSynI

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. SLIT1A-c003

    Plasmid

    #205124

    Purpose

    SLIT1 protein expression

    Depositor

    Paul Brennan

    Insert

    SLIT1 (SLIT1 Human)
    

    Use

    Tags

    AviTag-6His and PTPRSsp

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLenti-hSyn1-mClover3/CAAX

    Plasmid

    #197591

    Purpose

    Lentiviral transfer vector for the neuronal expression of membrane-bound mClover3 (good visualization of neuronal processes)

    Depositor

    Patrik Verstreken

    Insert

    mClover3-CAAX
    

    Use

    Lentiviral

    Tags

    CAAX

    Expression

    Mutation

    Promoter

    hSynI

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti-hSyn1-TagBFP2/CAAX

    Plasmid

    #197590

    Purpose

    Lentiviral transfer vector for the neuronal expression of membrane-bound TagBFP2 (good visualization of neuronal processes)

    Depositor

    Patrik Verstreken

    Insert

    mTagBFP2-CAAX
    

    Use

    Lentiviral

    Tags

    CAAX

    Expression

    Mutation

    Promoter

    hSynI

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJH200

    Plasmid

    #13327

    Depositor

    Martin Gellert

    Article

    Lieber et al Genes Dev. 1987 Oct . 1(8):751-61.

    Insert

    recombination substrate
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

35. pKS1531

    Plasmid

    #239521

    Purpose

    Express truncated VoGES and VvGT14 in the peroxisome for synthesis of geranyl _-glucoside; chromosomal integration by CRISPR

    Depositor

    John Dueber

    Insert

    tVoGES-ePTS1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pOpen-MMLV_RT (mut H)

    Plasmid

    #165546

    Purpose

    Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.

    Depositor

    FreeGenes Project, Jennifer Molloy, Drew Endy

    Insert

    Moloney Murine Leukemia Virus (MMLV) Reverse Transcriptase (RNAse H deactivated by 3 mutations)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 10, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

37. pLenti-hSyn1-mRuby2-T2A-GCaMP6f

    Plasmid

    #197595

    Purpose

    Lentiviral transfer vector for the neuronal expression of mRuby2-T2A-GCaMP6f (fluorescent reporter for Calcium imaging)

    Depositor

    Patrik Verstreken

    Insert

    mRuby2-T2A-GCaMP6f
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hSynI

    Available since

    April 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. TC9pAB

    Plasmid

    #71310

    Purpose

    used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI

    Depositor

    Karl Degenhardt

    Insert

    Cas9
    

    Use

    In vitro transcription

    Tags

    NLS

    Expression

    Mutation

    Promoter

    T7

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3_GMCL2-FLAG

    Plasmid

    #237344

    Purpose

    transient overexpression in mammalian cells

    Depositor

    Michele Pagano

    Insert

    GMCL2 (GMCL1P1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTW386c

    Plasmid

    #237321

    Purpose

    expresses O. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2

    Depositor

    Tina Wang

    Insert

    O. sativa Cpn60α Cpn60β Cpn20-2 Raf1 Raf2 RbcX Bsd2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge