We narrowed to 152,726 results for: Des

1. pDEST53-LRRK2-3XKD-R1441C

   Plasmid

   #25050

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   "Kinase Dead" Lysine 1906 mutated to A…

   Promoter

   Available since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pMMACE DEST CMV SpCas9

   Plasmid

   #206268

   Purpose

   DEST vector for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Encodes SpCas9 under the control of CMV promoter. CRE-Acceptor in MultiBac system.

   Depositor

   Imre Berger

   Article

   Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

   Insert

   SpCas9
   

   Use

   CRISPR; Recombinant baculovirus production

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDEST27-GST-ATXN1 FL(30Q)

   Plasmid

   #21753

   Depositor

   Huda Zoghbi

   Article

   Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

   Insert

   Ataxin-1 (ATXN1 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   insert contains a stretch of 32 amino acids (30 Q…

   Promoter

   CMV

   Available since

   July 18, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pDEST40-2XFL-Src-Y530EEI

   Plasmid

   #140323

   Purpose

   Expresses Flag-tagged human Src-Y530EEI in mammalian cells for structure-function studies

   Depositor

   Mark Moasser

   Article

   Spassov et al Cell Rep. 2018 Oct 9;25(2):449-463.e4. d

   Insert

   Src mutant (SRC Human)
   

   Use

   Tags

   2X Flag

   Expression

   Mammalian

   Mutation

   Y530EEI

   Promoter

   CMV

   Available since

   April 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE- TRE-DEST-CBioTAP

   Plasmid

   #53569

   Purpose

   Lentiviral destination vector for C-Bio-1xproteinA constructs using Gateway recombination System. Design for cDNA clones from the CCSBHuman Orfeome Collection.

   Depositor

   Mitzi Kuroda

   Article

   Alekseyenko et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. JH401: pMVP/Ad/RGD-PK-DEST

   Plasmid

   #121846

   Purpose

   pMVP destination vector, empty RGD/PK fiber-modified adenovirus vector backbone

   Depositor

   Christopher Newgard

   Article

   Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

   Type

   Empty backbone

   Use

   Adenoviral; Pmvp gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA 3.2/V5-DEST NET1A V5 WT

   Plasmid

   #69817

   Purpose

   Expresses NET1A-V5 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   NET1A (NET1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDest-1.7kbfabp6-eGFP-pA-CrymCherry

   Plasmid

   #159088

   Purpose

   LR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA inserts

   Depositor

   John Rawls

   Article

   Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

   Inserts

   1.7kb fabp6 promoter (fabp6 Zebrafish)
   eGFP
   polyA
   

   Use

   Fluorescent reporter

   Tags

   Expression

   Mutation

   Promoter

   1.7kb fabp6

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA-Dest47- WTN23C11orf83-GFP

   Plasmid

   #65845

   Purpose

   Mammalian expression of the wild type N terminal part (N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)

   Depositor

   Amos Bairoch

   Article

   Desmurs et al Mol Cell Biol. 2015 Apr;35(7):1139-56. d

   Insert

   Transmembrane (23 AA) of UQCC3 (UQCC3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   WT Transmembrane

   Promoter

   Available since

   July 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDest-302 (attR4-attR2)

    Plasmid

    #161894

    Purpose

    Multisite Gateway (attR4-attR2) vector for mammalian protein expression

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDest-312 (attR4-attR3)

    Plasmid

    #161897

    Purpose

    Multisite Gateway (attR4-attR3) vector for mammalian protein expression

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAD/CMV/V5-DEST-Rac1-2G

    Plasmid

    #66112

    Purpose

    Second generation Rac1 FRET biosensor for adenovirus production

    Depositor

    Olivier Pertz

    Article

    Fritz et al Dev Cell. 2015 Sep 29. pii: S1534-5807(1

    Insert

    Second generation Rac1 FRET biosensor
    

    Use

    Adenoviral

    Tags

    His-Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEF-DEST51 GFP-PNRC1

    Plasmid

    #123295

    Purpose

    Mammalian expression of PNRC1 N-GFP

    Depositor

    Giovanni Tonon

    Article

    Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

    Insert

    PNRC1 (PNRC1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. p6272 pDEST-60-GST-HPV16E6

    Plasmid

    #24127

    Depositor

    Peter Howley

    Insert

    HPV16 E6 (E6, E6 HPV)
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    none

    Promoter

    Available since

    April 2, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pDEST-hMeCP2-GFP-G273X

    Plasmid

    #48080

    Purpose

    Mammalian expression of GFP tagged MeCP2-G273X

    Depositor

    Huda Zoghbi

    Article

    Baker et al Cell. 2013 Feb 28;152(5):984-96. doi: 10

    Insert

    Methyl-CpG Binding Protein 2 (Rett Syndrome) (MECP2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    expresses AT-Hook G273X

    Promoter

    CMV

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST15.1-hRPN13 (285-407)

    Plasmid

    #61934

    Purpose

    Expression of hRPN13 (285-407) in E.coli

    Depositor

    Chris Hill

    Article

    VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

    Insert

    hRPN13 (285-407) (ADRM1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Construct starts at residue 285

    Promoter

    T7

    Available since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

17. JP705: pMVP/SB/Neo-DEST

    Plasmid

    #121866

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Neo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEYFP-Pds1Δdestruction box(383)

    Plasmid

    #39848

    Depositor

    Jonathon Pines

    Article

    Hagting et al J Cell Biol. 2002 Jun 24;157(7):1125-37.

    Insert

    Pds1 (PTTG1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Δdestruction box = ΔR61-N68

    Promoter

    Available since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDEST-actc1b-ccdb-mKate2

    Plasmid

    #109731

    Purpose

    Multisite gateway destination vector for muscle specific expression of constructs tagged at the 3' end with mKate2

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Type

    Empty backbone

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDEST-actc1b-mKate2-ccdb

    Plasmid

    #109732

    Purpose

    Multisite gateway destination vector for muscle specific expression of constructs tagged at the 5' end with mKate2

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Type

    Empty backbone

    Use

    Zebrafish plasmids

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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