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pDEST-FRT/T0-Flag-PALB2

Plasmid

#71114

Purpose

mammalian expession of FLAG-PALB2

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV/TO

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-DEST53 PINK1 N-GFP

Plasmid

#13315

Depositor

Mark Cookson

Article

Beilina et al Proc Natl Acad Sci U S A. 2005 Apr 19. 1

Insert

PINK1 (PINK1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pDEST-Myc-FBW7]K404R

Plasmid

#200715

Purpose

The plasmid expresses Myc-tagged FBW7 human isoform 1 with Lys404 to Arg mutation. Used for overexpression and detection by western blotting.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 (FBXW7 Human)

Tags

Myc

Expression

Mammalian

Mutation

FBW7 K404 is mutated to Alanine.

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pDest-1.7kbfabp6-eGFP-pA-CrymCherry

Plasmid

#159088

Purpose

LR construct using backbone with Cry:mCherry insert to identify fish with transgene, and with p5E-1.7kbfabp6, pME-eGFP, and p3E-pA inserts

Depositor

John Rawls

Article

Wen et al Sci Adv. 2021 Jul 23;7(30). pii: 7/30/ea

Inserts

1.7kb fabp6 promoter (fabp6 Zebrafish)
eGFP
polyA

Use

Fluorescent reporter

Promoter

1.7kb fabp6

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/TO/V5-DEST-Lifeact-mScarlet-I

Plasmid

#225495

Purpose

Expression of Lifeact-mScarlet-I (lentiviral vector)

Depositor

Guillaume Jacquemet

Article

Follain et al EMBO J. 2026 Jan 6. doi: 10.1038/s44318-

Insert

Lifeact

Use

Lentiviral

Tags

mScarlet-I

Expression

Mammalian

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pDestTol2-actinb-kcnj13-EGFP

Plasmid

#164940

Purpose

Tol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 fused with EGFP at the C terminus

Depositor

GuangJun Zhang

Article

Silic et al Genetics. 2020 Aug;215(4):1067-1084. doi

Insert

kcnj13 (kcnj13 Zebrafish)

Use

Tol2 transposon destination vector

Tags

EGFP

Mutation

Stop codon deleted

Promoter

actinb (actb2) promoter

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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HA-BirA*-pDEST-N-pcDNA5/FRT/TO

Plasmid

#118375

Purpose

Expression vector for BioID in mammalian system. Gateway compatible destination vector to generate stable, inducible cell line expressing N-terminally tagged HABirA* and protein of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

HA-BirA*

Expression

Mammalian

Available Since

Nov. 30, 2018

Availability

Academic Institutions and Nonprofits only

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GLUT1-eGFP/pcDNA-DEST47

Plasmid

#18729

Depositor

Wolf Frommer

Article

Takanaga et al Biochim Biophys Acta. 2008 Apr . 1778(4)

Insert

pcDNA-DEST47 /hGLUT1-eGFP

Tags

GFP

Expression

Mammalian

Mutation

deleted TAA

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pDEST-GST-FBW7 alpha

Plasmid

#197456

Purpose

The plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 (FBXW7 Human)

Use

In vitro translation

Tags

GST

Expression

Bacterial

Mutation

See depositor comments below

Promoter

T7

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST MTFR1L-V5

Plasmid

#69822

Purpose

Expresses MTFR1L-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Mitochondrial fission regulator 1-like (MTFR1L Human)

Use

Retroviral

Tags

v5

Expression

Mammalian

Promoter

cmv

Available Since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST CDC42EP1 WT

Plasmid

#69819

Purpose

Expresses CDC42EP1-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

CDC42 effector protein (CDC42EP1 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-mCherry-LacR-BRCA1

Plasmid

#71115

Purpose

mammalian expression of mCherry-LacR-BRCA1 siRNA resistant construct

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

BRCA1 (BRCA1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV/TO

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST PUM2

Plasmid

#40292

Depositor

Thomas Tuschl

Article

Hafner et al Cell. 2010 Apr 2;141(1):129-41.

Insert

Pumilio homolog 2 (PUM2 Human)

Tags

FLAG-HA

Expression

Mammalian

Available Since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pCB59 pDEST crybb1:mKate2

Plasmid

#195986

Purpose

Transgenesis backbone

Depositor

Markus Affolter, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Nst-CAG-DEST

Plasmid

#80489

Purpose

Gateway Destination donor vector for AAVS1 targeting (puromycin selection)

Depositor

Knut Woltjen

Article

Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

Type

Empty backbone

Use

Donor vector (human)

Expression

Mammalian

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pDEST-mCherry-LacR-PALB2

Plasmid

#71112

Purpose

mammalian expession of mCherry-LacR-PALB2

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV/TO

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti X2 Blast DEST (w46-2)

Plasmid

#17391

Purpose

3rd gen lentiviral Gateway destination vector, RNAi, 3’LTR insertion, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning, Lentiviral, and RNAi

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pDestTol2CG2-mnx1:ReaChR-citrine

Plasmid

#169046

Purpose

Destination transgenesis vector for expressing ReaChR fused with citrine in zebrafish spinal motor neurons using 3 tandem repeats of the mnx1 enhancer.

Depositor

Paola Arlotta

Article

Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

Insert

ReaChR-Citrine

Tags

Citrine

Expression

Mammalian

Promoter

3xMnx1

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-FRT/T0-GFP-PALB2

Plasmid

#71113

Purpose

mammalian expession of GFP-PALB2

Depositor

Daniel Durocher

Article

Orthwein et al Nature. 2015 Dec 9. doi: 10.1038/nature1

Insert

PALB2 (PALB2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV/TO

Available Since

Dec. 9, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.2 V5-DEST mir-1-1 reporter

Plasmid

#46646

Purpose

expression of query pri-miRNA

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir-1-1 reporter

Use

RNAi; Expression of query pri-mirna sequences in …

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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