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We narrowed to 6,952 results for: crispr cas9 plasmids

Showing: 1901 - 1920 of 6952 results

  1. pZNF75A.1.0-gDNA

    Plasmid
    #112403
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ZNF75A
    Depositor
    Kevin White
    Insert
    ZNF75A (ZNF75A Human)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112403-plasmid-map-sequence-id-230258

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  3. pFC902

    Plasmid
    #106904
    Purpose
    The vector has a U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA-Gly repeat; and a gene encoding cas9 controlled by Ptef1 and Ttef1
    Depositor
    Uffe Mortensen
    Article
    Nodvig et al Fungal Genet Biol. 2018 Jan 8. pii: S108
    Inserts
    cas9
    pyrG
    U3 promoter and terminator controlled gene encoding a transcript containing the sgRNA flanked by a tRNA gly repeat
    Use
    CRISPR; Fungal expression
    Tags
    SV40 NLS
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    106904-plasmid-map-sequence-id-206325

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  4. MSP1101

    Plasmid
    #65773
    Purpose
    Human expression vector for SpCas9 VRER variant: CMV-T7-humanSpCas9(D1135V/G1218R/R1335E/T1337R)-NLS-3xFLAG (VRER variant)
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/G1218R/R1335E/T1337R)-NLS-3XFlag
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Mammalian
    Mutation
    D1135V, G1218R, R1335E, and T1337R mutations in C…
    Promoter
    CMV & T7
    Available since
    June 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65773-plasmid-map-sequence-id-112657

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  5. pAAV-CMV-dSa VP64 Actc1

    Plasmid
    #99691
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Actc1 promoter, vector allows for activation of mouse Actc1, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG) (Actc1 Synthetic, Mouse)
    Use
    AAV and CRISPR
    Tags
    VP64
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Dec. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    99691-plasmid-map-sequence-id-194530

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  6. pAAV-CMV-dSa VP64 Neurog2

    Plasmid
    #99695
    Purpose
    Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Neurog2 promoter, vector allows for activation of mouse Neurog2, can be packaged and delivered as AAV
    Depositor
    George Church
    Article
    Vora et al bioRxiv 2018. 298620
    Insert
    dCas9 and gRNA targeting Neurog2 (gRNA: GGTATATAAGGGGTTTTAAG) (Neurog2 Synthetic, Mouse)
    Use
    AAV and CRISPR
    Tags
    VP64
    Expression
    Mutation
    dead Cas9
    Promoter
    Available since
    Sept. 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    99695-plasmid-map-sequence-id-194570

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  7. pHSN401

    Plasmid
    #50588
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (AtU6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Expression
    Mutation
    maize codon optimized
    Promoter
    2×35Sp and AtU6-26p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50588-plasmid-map-sequence-id-75660

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  8. pBUN411

    Plasmid
    #50581
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (OsU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    3×FLAG-NLS-zCas9-NLS
    gRNA scaffold
    Use
    CRISPR; Plant expression
    Tags
    3xFLAG and NLS
    Expression
    Mutation
    maize codon optimized
    Promoter
    OsU3p and Ubi1p
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50581-plasmid-map-sequence-id-311045

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  9. MSP2283

    Plasmid
    #70702
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70702-plasmid-map-sequence-id-127769

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  10. MSP2266

    Plasmid
    #70705
    Purpose
    Bacterial expression plasmid for SaCas9 & sgRNA targeted to site 2: T7-humanSaCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038
    Inserts
    mammalian codon-optimized Staphylococcus aureus Cas9
    SaCas9 sgRNA (+84)
    Use
    CRISPR
    Tags
    NLS-3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Dec. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    70705-plasmid-map-sequence-id-131909

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  11. pCY5.1nh

    Plasmid
    #160299
    Purpose
    Yeast CRISPR plasmid targeting the natMX and hphMX cassettes
    Depositor
    Amir Aharoni
    Article
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting hph: GACCTGATGCAGCTCTCGGA)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160299-plasmid-map-sequence-id-464600

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  12. pCY5.1kn

    Plasmid
    #160298
    Purpose
    Yeast CRISPR plasmid targeting the kanMX and natMX cassettes
    Depositor
    Amir Aharoni
    Article
    Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do
    Inserts
    sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
    URA3MX selection cassette
    sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
    Cas9
    Use
    CRISPR
    Tags
    8xHis-tag and SV40 NLS
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    160298-plasmid-map-sequence-id-464598

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Showing: 1901 - 1920 of 6952 results