165,545 results

1. AiP1427 - pAAV-AiE2135m-minBG-SYFP2-WPRE-BGHpA

   Plasmid

   #214497

   Purpose

   AiE2135m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   The Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pYPQ141D2.0

   Plasmid

   #99906

   Purpose

   Express single gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

   Depositor

   Yiping Qi

   Article

   Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   OsU3

   Available since

   March 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. R777-E285 Hs.ROCK2

   Plasmid

   #70569

   Purpose

   Gateway ORF clone of human ROCK2 [NM_004850.3] with stop codon (for native or N-terminal fusions)

   Depositor

   Dominic Esposito

   Insert

   ROCK2 (ROCK2 Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3 basic E2

   Plasmid

   #48747

   Purpose

   Expression of luciferase driven by mPer2 promoter fragment containing E-box2 (bases -112 to +98 with respect to transcription start site at +1)

   Depositor

   Joseph Takahashi

   Article

   Yoo et al Proc Natl Acad Sci U S A. 2005 Feb 15;10

   Insert

   mPer2 promoter/enhancer region containing E-box2 (-112 to +98)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX-4T1_ATG4B_BamHI_NotI

   Plasmid

   #190862

   Purpose

   Plasmid for the expression and purification of of ATG4B. Internal reference: SMC1392

   Depositor

   Sascha Martens

   Article

   Fracchiolla et al J Cell Biol. 2020 Jul 6;219(7). pii: 151

   Insert

   ATG4B (ATG4B Human)
   

   Use

   Tags

   GST, Thrombin Cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFA6a-6xGLY-Myc-kanMX6

   Plasmid

   #20769

   Depositor

   Mark Hochstrasser

   Article

   Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

   Type

   Empty backbone

   Use

   Pcr-based c-terminal tagging

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   March 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV.CAGFLEX.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209657

   Purpose

   Expression of iATPSnFR2, low affinity

   Depositor

   HHMI-JRC Tool Translation Team, Tim Brown

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   AAV

   Tags

   Expression

   Mutation

   A95A.A119L

   Promoter

   CAG

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSLQ10704

   Plasmid

   #183957

   Purpose

   U6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFP

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   SKSKSO array
   

   Use

   Lentiviral

   Tags

   EF1a-Puro-T2A-TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. HaloTag-LaminA

   Plasmid

   #231894

   Purpose

   Expression of HaloTag-tagged LaminA to visualize the nuclear lamina

   Depositor

   Lukas Kapitein

   Article

   Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

   Insert

   LaminA (LMNA Human)
   

   Use

   Tags

   HaloTag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMK382

    Plasmid

    #214373

    Purpose

    Tet-OsTIR1(F74G) (AAVS1 insertion donor)

    Depositor

    Masato Kanemaki

    Article

    Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

    Insert

    Tet-OsTIR1(F74G)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet ON 3G promoter

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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