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We narrowed to 123,065 results for: CIL

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Showing: 1921 - 1940 of 123065 results

  3. pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)

    Plasmid
    #133791
    Purpose
    U6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    St1Cas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    V1 sgRNA architecture from Carter et al. biorxiv …
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133791-plasmid-map-sequence-id-268588

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  4. pBT3038

    Plasmid
    #122545
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a lysosomal related organelle localized gene (glo-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_glo-1::CemOrange2 (glo-1 Synthetic, Nematode)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122545-plasmid-map-sequence-id-238133

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  5. pBT3043

    Plasmid
    #122546
    Purpose
    Expression of a C. elegan codon optimized florescent protein (CemOrange2) fused to a autophagic localized gene that is orthologus to mamalian LC3 (sqst-1) under the intestinal specific promoter nhx-2
    Depositor
    Gary Silverman
    Article
    Thomas et al PLoS One. 2019 Mar 26;14(3):e0214257. do
    Insert
    Pnhx-2_CemOrange2::lgg-1 (lgg-1 Synthetic, Nematode)
    Expression
    Bacterial
    Promoter
    Pnhx-2
    Available Since
    March 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    122546-plasmid-map-sequence-id-238140

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  6. CoV2-Spike-EF1a-D614G-N501Y

    Plasmid
    #177939
    Purpose
    Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    SARS-CoV-2 Spike protein (D614G, N501Y) (S )
    Expression
    Mammalian
    Mutation
    D614G, N501Y
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177939-plasmid-map-sequence-id-348111

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  7. pThermoCas9_ctrl

    Plasmid
    #100981
    Purpose
    Expresses ThermoCas9 and its sgRNA module
    Depositor
    John van der Oost
    Article
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available Since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100981-plasmid-map-sequence-id-196605

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  11. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

    Plasmid
    #133794
    Purpose
    U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    NmeCas9 and Nme2Cas9 sgRNA entry vector
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Amrani et al. Genome Biol…
    Promoter
    U6
    Available Since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133794-plasmid-map-sequence-id-268076

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  20. pTEF:ATP

    Plasmid
    #92179
    Purpose
    HO-TEF1p-ATeam1.03-KanMX4-HO. Codon optimized for yeast version of the ATeam1.03 ATP FRET sensor (originally from Imamura et al. PNAS, 2009 ), expressed via the TEF1 promoter. HO locus integration.
    Depositor
    Matthias Heinemann
    Article
    Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi
    Insert
    ATeam1.03 codon optimized for yeast (Saccharomyces cerevisiae)
    Use
    Integration and expression into yeast.
    Tags
    Yeast codon optimized mseCFP (donor) and cp173-mV…
    Expression
    Yeast
    Promoter
    TEF1p
    Available Since
    June 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    92179-plasmid-map-sequence-id-181610

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Showing: 1921 - 1940 of 123065 results