We narrowed to 3,906 results for: SPL;

1. (302) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5)

   Plasmid

   #168192

   Purpose

   HA-tagged GFP nanobody Lag6 (310 nM affinity) fused to a truncated OGT of 4 TPRs

   Depositor

   Christina Woo

   Article

   Ramirez et al Curr Protoc. 2021 May;1(5):e117. doi: 10

   Insert

   HA-LaG6-(EAAAK)4-OGT(4)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. MBP-mmA1

   Plasmid

   #61090

   Purpose

   expression of fusion protein: MBP-hnRNP A1

   Depositor

   Sean Ryder

   Article

   Zearfoss et al RNA. 2013 Jul;19(7):948-57. doi: 10.1261

   Insert

   hnRNP A1 (Hnrnpa1 Mouse)
   

   Use

   Tags

   H6_MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pAW-AlphaLobe

   Plasmid

   #113255

   Purpose

   E. coli expression vector for His-MBP-Cas9 Alpha-Helical Lobe

   Depositor

   Jennifer Doudna

   Article

   Wright et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   S. pyogenes Cas9 alpha-helical lobe
   

   Use

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Mutation

   residues 56–714; See paper for description

   Promoter

   T7

   Available since

   Aug. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSiMPlc_N + pSiMPlc_C

   Plasmid

   #134312

   Purpose

   Maintenance of 2 plasmids with a single antibiotic in E. coli

   Depositor

   Barbara Di Ventura

   Article

   Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

   Insert

   cmR_gp41-1 N-intein + cmR_gp41-1 C-intein
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-SUN1_785

   Plasmid

   #125852

   Purpose

   Expresses SUN1_785 tagged with GFP in mammalian cells

   Depositor

   Miki Hieda

   Article

   Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

   Insert

   SUN1_785 (SUN1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLHCX-FLAG-mPKM1

   Plasmid

   #44240

   Depositor

   Matthew Vander Heiden, Lewis Cantley

   Article

   Christofk et al Nature. 2008 Mar 13;452(7184):230-3. doi

   Insert

   Pyruvate Kinase M1 (Pkm Mouse)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 10, 2013

   Availability

   Academic Institutions and Nonprofits only

7. sABE v3.22 Lenti_2

   Plasmid

   #209556

   Purpose

   pU6-SpCas9_sgRNA-pCMV-bpSV40_NLS-TadA-8e (2-76)-2xFRB

   Depositor

   Xue Gao

   Article

   Zeng et al Nat Commun. 2023 Sep 11;14(1):5573. doi:

   Insert

   SpCas9 sgRNA, TadA-8e (2-76)-2xFRB
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   pU6, pCMV

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAtetO_6xHis-v5.9

   Plasmid

   #155259

   Purpose

   Plasmid for the aTc-inducible expression of the v5.9

   Depositor

   Andrew Ellington

   Article

   Milligan et al Biochemistry. 2018 Aug 7;57(31):4607-461

   Insert

   v5.9
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   tet PA

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. tol2-CAG::ChR2-YFP

   Plasmid

   #59740

   Purpose

   Optogenetic excitation of ES cell-derived neurons (and other cells, such as muscle)

   Depositor

   Ivo Lieberam

   Article

   Bryson et al Science. 2014 Apr 4;344(6179):94-7. doi:

   Insert

   Channelrhodopsin-2
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   CAG

   Available since

   Oct. 2, 2014

   Availability

   Academic Institutions and Nonprofits only

10. pACUH-sfcherry2(1-10)

    Plasmid

    #172061

    Purpose

    UAS vector to express sfcherry2(1-10) in Drosophila

    Depositor

    Daichi Kamiyama

    Article

    Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

    Insert

    sfcherry2(1-10)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA5-FRT-TetO-U2B''-fSNAPc

    Plasmid

    #106988

    Purpose

    open reading frame of U2B'' inserted into pcDNA5-FRT-TetO-fSNAPc using the KpnI and NotI restriction sites

    Depositor

    Melissa Moore

    Article

    Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

    Insert

    UB2" (SNRPB2 Human)
    

    Use

    Tags

    fSNAP

    Expression

    Mutation

    Promoter

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG CAG Glucagon-ICAM1-3xHA-pre-mGRASP

    Plasmid

    #153008

    Purpose

    Expresses glucagon peptide on mammalian cell surface

    Depositor

    Alice Ting

    Article

    Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

    Insert

    Glucagon-ICAM1-pre-mGRASP
    

    Use

    Tags

    3x HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PEP110E

    Plasmid

    #97396

    Purpose

    binary construct of nuclear mCherry with sfGFP11 tag for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    mCherry-sfGFP11
    

    Use

    Tags

    Nucleus target

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-SUN1_888

    Plasmid

    #125851

    Purpose

    Expresses SUN1_888 tagged with GFP in mammalian cells

    Depositor

    Miki Hieda

    Article

    Nishioka et al Nucleus. 2016 Nov;7(6):572-584. doi: 10.

    Insert

    SUN1_888 (SUN1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLSU4cg10

    Plasmid

    #126078

    Purpose

    Plant transformation vector for stable expression of plastid targeted GFP1-10 in plant cells

    Depositor

    Ralf-Bernd Klösgen

    Article

    Sharma et al J Cell Sci. 2019 Jun 3;132(11). pii: jcs

    Insert

    Chloroplastic FNR transit peptide
    

    Use

    Binary

    Tags

    GFP1-10

    Expression

    Plant

    Mutation

    Promoter

    longCaMV35S

    Available since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPRv2-TRAF6-targeting sgRNA 2

    Plasmid

    #131346

    Purpose

    gRNA targeting mouse TRAF6

    Depositor

    Jonathan Kagan

    Article

    Tan et al Cell. 2019 Apr 4;177(2):384-398.e11. doi

    Insert

    gRNA targeting mouse TRAF6 (Traf6 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. IBMc460

    Plasmid

    #161940

    Purpose

    Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PPhlF

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMC051-HisMS2_PLP_pac_LC

    Plasmid

    #128234

    Purpose

    Lower Copy expression construct for MS2 phage like particles with Esp3I cloning sites for packaged RNA.

    Depositor

    Paul Freemont

    Article

    de Martin Garrido et al Mol Microbiol. 2019 Oct 16. doi: 10.1111

    Inserts

    Maturation Protein
    Coat Protein Dimer
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 15, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pcDNA3.1 FLIPE-1m

    Plasmid

    #13544

    Depositor

    Wolf Frommer

    Article

    Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

    Insert

    FLIP-E A207W
    

    Use

    Tags

    ECFP and Venus

    Expression

    Mammalian

    Mutation

    A207W in binding protein

    Promoter

    Available since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CamKIIa-moxBFP-IRES-Flpo

    Plasmid

    #194977

    Purpose

    AAV vector to drive expression of moxBFP and Flpo under the control of CamKIIalpha promoter

    Depositor

    Günter Schwarz

    Article

    Liebsch et al eNeuro. 2023 Jan 4;10(1):ENEURO.0102-22.

    Insert

    moxBFP, Flpo
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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