311,568 results

1. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

   Plasmid

   #139090

   Purpose

   Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   dSpCas9-VPR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   Available since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-hARdelta538-614

   Plasmid

   #89107

   Purpose

   mammalian expression of human androgen receptor with deletion of amino acids 538-614, deletion of AR DNA binding domain

   Depositor

   Elizabeth Wilson

   Article

   Zhou et al J Biol Chem. 1994 May 6;269(18):13115-23

   Insert

   hAR-538-614 (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAR delta 538-614

   Promoter

   CMV

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

3. pMRX-IPU-GFP-MYH9

   Plasmid

   #168273

   Purpose

   Expresses MYH9 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Yamamoto et al Nat Commun. 2021 Jun 2;12(1):3292. doi:

   Insert

   MYH9 (MYH9 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLKO.3G

   Plasmid

   #14748

   Depositor

   Diane Mathis, Christophe Benoist

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 5, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pMRE-Tn7-163

   Plasmid

   #118575

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   sYFP2
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. LSL-Cas9-Rosa26TV

   Plasmid

   #61408

   Purpose

   Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   Cas9
   EGFP
   

   Use

   CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   3xFLAG and P2A

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CAG

   Available since

   Jan. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAcGFP1-N1-SialT

   Plasmid

   #87324

   Purpose

   To express SialT-AcGFP1 in mammalian cells, SialT encodes the full length coding sequence of β-galactosamide α-2,6-sialyltransferase

   Depositor

   Lei Lu

   Article

   Tie et al Mol Biol Cell. 2016 Jan 13. pii: mbc.E15

   Insert

   ST 6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1 Human)
   

   Use

   Tags

   AcGFP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-OptoSTIM1

   Plasmid

   #70159

   Purpose

   Mammalian expression plasmid of OptoSTIM1 (optogenetically-activated STIM1 protein)

   Depositor

   Won Do Heo

   Article

   Kyung et al Nat Biotechnol. 2015 Sep 14. doi: 10.103

   Insert

   OptoSTIM1 (STIM1 Mustard Weed, Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-hAR-H874Y

   Plasmid

   #89087

   Purpose

   mammalian expression of human androgen receptor with prostate cancer somatic mutation H874Y from CWR22 xenograft

   Depositor

   Elizabeth Wilson

   Article

   Tan et al Mol Endocrinol. 1997 Apr;11(4):450-9. do

   Insert

   hAR-H874Y prostate cancer mutant (AR Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   hAR-H874Y

   Promoter

   CMV

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

10. TFORF1526

    Plasmid

    #144182

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    MXI1 (MXI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge