We narrowed to 25,186 results for: spr
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Plasmid#126484PurposeEncodes L.monocytogenes CRISPR Type I-B Cas5 with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertLmoCas5
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMV and U6Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hMb3Cas12a-NLS(nucleoplasmin)-3xHA (RTW2500)
Plasmid#115142PurposeCAG promoter expression plasmid for human codon optimized Mb3Cas12a nuclease with C-terminal NLS and HA tagDepositorInserthuman codon optimized Mb3Cas12a
TagsNLS(nucleoplasmin) and 3xHAExpressionMammalianAvailable SinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-MS2
Plasmid#184839PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterJ23119 and lac promoterAvailable SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUDP012
Plasmid#101167PurposeE. coli/S. cerevisiae shuttle vector carrying amdS andSpcas9D147Y P411T and expressing a ribozyme flanked g-RNA for Cas9 editing targeting the gene SeILV6 and in S. pastorianus (HH-gRNASeILV6-HDV)DepositorInsertHH-gRNA-HDV targetting SeILV6 in S. pastorianus
UseCRISPRExpressionYeastPromoterScTDH3Available SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPPH-T2A-GFP-IRES-Neo
Plasmid#102892PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-P300-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo
Plasmid#102891PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-P300-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCas6
Plasmid#106274PurposeEncodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasE
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMVAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-LmoCas6-KRAB-2a-BlastR
Plasmid#126492PurposeEncodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoterDepositorInsertLmoCas6-KRAB-2a-BlastR
UseCRISPR and LentiviralTagsFlag, NLSExpressionMammalianPromoterhUbCAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCas8e
Plasmid#106270PurposeEncodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasA
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMVAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCse2
Plasmid#106271PurposeEncodes human codon optimized E.coli CRISPR Type I-E CasB with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasB
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMVAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCas7
Plasmid#106272PurposeEncodes human codon optimized E.coli CRISPR Type I-E CasC with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasC
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMVAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-EcoCas5
Plasmid#106273PurposeEncodes human codon optimized E.coli CRISPR Type I-E CasD with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoterDepositorInsertEcoCasD
UseCRISPRTagsFlag, NLSExpressionMammalianPromoterCMVAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS
Plasmid#113437PurposeExpresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cellsDepositorInsertType II-C anti-CRISPR
UseCRISPRTagsFLAG/NLSExpressionMammalianPromoterCMVAvailable SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
H1_sgRNA(GRIN2B)_SauCas9
Plasmid#246054PurposeExpression of a sgRNA targeting the GRIN2B locus and SauCas9 under the same pol-III H1 promoterDepositorInsertSauCas9 and sgRNA(GRIN2B)
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable SinceDec. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_AcrIIC3_cpGR2(GGS5)-F59
Plasmid#246053PurposeExpression of AcrIIC3-cpGR2 hybrid in mammalian cells; insertion before F59; enables chemogenetic control of NmeCas9DepositorInsertAcrIIC3_cpGR2-F59
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable SinceDec. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_AcrIIA5_cpGR2(GGS5)-N77
Plasmid#246046PurposeExpression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before N77; enables chemogenetic control of SauCas9DepositorInsertAcrIIA5_cpGR2-N77
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable SinceDec. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_AcrIIA5_cpGR2(GGS5)-D41
Plasmid#246045PurposeExpression of AcrIIA5-cpGR2 hybrid in mammalian cells; insertion before D41; enables chemogenetic control of SauCas9DepositorInsertAcrIIA5_cpGR2-D41
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable SinceDec. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_AcrIIA5_AsLOV2-N77
Plasmid#246044PurposeExpression of AcrIIA5-AsLOV2 hybrid in mammalian cells; insertion before N77; enables optogenetic control of SauCas9DepositorInsertAcrIIA5_AsLOV2-N77
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable SinceDec. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
CMV_AcrIIA5_AsLOV2-D41
Plasmid#246043PurposeExpression of AcrIIA5-AsLOV2 hybrid in mammalian cells; insertion before D41; enables optogenetic control of SauCas9DepositorInsertAcrIIA5_AsLOV2-D41
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable SinceDec. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pWT-IFl_WT-IFr
Plasmid#192207PurposeWildtype I-F CRISPR Leader and a CRISPR locus with two wildtype I-F repeats, for integration assays.DepositorInsertWildtype I-F Leader and wildtype I-F Locus DNA
ExpressionBacterialAvailable SinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only